MALT1 (NM_173844) Human Tagged ORF Clone

CAT#: RC204322

MALT1 (Myc-DDK-tagged)-Human mucosa associated lymphoid tissue lymphoma translocation gene 1 (MALT1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY406281) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204322 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MALT1 protein (Cat# TP304322). The protein was produced from HEK293T cells transfected with MALT1 cDNA clone (Cat# RC204322) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MALT1
• Synonyms: IMD12; MLT; MLT1; PCASP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204322 representing NM_173844
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCTGTTGGGGGACCCGCTACAGGCCCTGCCGCCCTCGGCCGCCCCCACGGGGCCGCTGCTCGCCC
  CTCCGGCCGGCGCGACCCTCAACCGCCTGCGGGAGCCGCTGCTGCGGAGGCTCAGCGAGCTCCTGGATCA
  GGCGCCCGAGGGCCGGGGCTGGAGGAGACTGGCGGAGCTGGCGGGGAGTCGCGGGCGCCTCCGCCTCAGT
  TGCCTAGACCTGGAGCAGTGTTCTCTTAAGGTACTGGAGCCTGAAGGAAGCCCCAGCCTGTGTCTGCTGA
  AGTTAATGGGTGAAAAAGGTTGCACAGTCACAGAATTGAGTGATTTCCTGCAGGCTATGGAACACACTGA
  AGTTCTTCAGCTTCTCAGCCCCCCAGGAATAAAGATTACTGTAAACCCAGAGTCAAAGGCAGTCTTGGCT
  GGACAGTTTGTGAAACTGTGTTGCCGGGCAACTGGACATCCTTTTGTTCAATATCAGTGGTTCAAAATGA
  ATAAAGAGATTCCAAATGGAAATACATCAGAGCTTATTTTTAATGCAGTGCATGTAAAAGATGCAGGCTT
  TTATGTCTGTCGAGTTAATAACAATTTCACCTTTGAATTCAGCCAGTGGTCACAGCTGGATGTTTGCGAC
  ATCCCAGAGAGCTTCCAGAGAAGTGTTGATGGCGTCTCTGAATCCAAGTTGCAAATCTGTGTTGAACCAA
  CTTCCCAAAAGCTGATGCCAGGCAGCACATTGGTTTTACAGTGTGTTGCTGTTGGAAGCCCTATTCCTCA
  CTACCAGTGGTTCAAAAATGAATTACCATTAACACATGAGACCAAAAAGCTATACATGGTGCCTTATGTG
  GATTTGGAACACCAAGGAACCTACTGGTGTCATGTATATAATGATCGAGACAGTCAAGATAGCAAGAAGG
  TAGAAATCATCATAGATGAATTAAATAATCTTGGTCATCCTGATAATAAAGAGCAAACAACTGACCAGCC
  TTTGGCGAAGGACAAGGTTGCCCTTTTGATAGGAAATATGAATTACCGGGAGCACCCCAAGCTCAAAGCT
  CCTTTGGTGGATGTGTACGAATTGACTAACTTACTGAGACAGCTGGACTTCAAAGTGGTTTCACTGTTGG
  ATCTTACTGAATATGAGATGCGTAATGCTGTGGATGAGTTTTTACTCCTTTTAGACAAGGGAGTATATGG
  GTTATTATATTATGCAGGACATGGTTATGAAAATTTTGGGAACAGCTTCATGGTCCCCGTTGATGCTCCA
  AATCCATATAGGTCTGAAAATTGTCTGTGTGTACAAAATATACTGAAATTGATGCAAGAAAAAGAAACTG
  GACTTAATGTGTTCTTATTGGATATGTGTAGGAAAAGAAATGACTACGATGATACCATTCCAATCTTGGA
  TGCACTAAAAGTCACCGCCAATATTGTGTTTGGATATGCCACGTGTCAAGGAGCAGAAGCTTTTGAAATC
  CAGCATTCTGGATTGGCAAATGGAATCTTTATGAAATTTTTAAAAGACAGATTATTAGAAGATAAGAAAA
  TCACTGTGTTACTGGATGAAGTTGCAGAAGATATGGGTAAGTGTCACCTTACCAAAGGCAAACAGGCTCT
  AGAGATTCGAAGTAGTTTATCTGAGAAGAGAGCACTTACTGATCCAATACAGGGAACAGAATATTCTGCT
  GAATCTCTTGTGCGGAATCTACAGTGGGCCAAGGCTCATGAACTTCCAGAAAGTATGTGTCTTAAGTTTG
  ACTGTGGTGTTCAGATTCAATTAGGATTTGCAGCTGAGTTTTCCAATGTCATGATCATCTATACAAGTAT
  AGTTTACAAACCACCGGAGATAATAATGTGTGATGCCTACGTTACTGATTTTCCACTTGATCTAGATATT
  GATCCAAAAGATGCAAATAAAGGCACACCTGAAGAAACTGGCAGCTACTTGGTATCAAAGGATCTTCCCA
  AGCATTGCCTCTATACCAGACTCAGTTCACTGCAAAAATTAAAGGAACATCTAGTCTTCACAGTATGTTT
  ATCATATCAGTACTCAGGATTGGAAGATACTGTAGAGGACAAGCAGGAAGTGAATGTTGGGAAACCTCTC
  ATTGCTAAATTAGACATGCATCGAGGTTTGGGAAGGAAGACTTGCTTTCAAACTTGTCTTATGTCTAATG
  GTCCTTACCAGAGTTCTGCAGCCACCTCAGGAGGAGCAGGGCATTATCACTCATTGCAAGACCCATTCCA
  TGGTGTTTACCATTCACATCCTGGTAATCCAAGTAATGTTACACCAGCAGATAGCTGTCATTGCAGCCGG
  ACTCCAGATGCATTTATTTCAAGTTTCGCTCACCATGCTTCATGTCATTTTAGTAGAAGTAATGTGCCAG
  TAGAGACAACTGATGAAATACCATTTAGTTTCTCTGACAGGCTCAGAATTTCTGAAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204322 representing NM_173844
  Red=Cloning site Green=Tags(s)
  
  MSLLGDPLQALPPSAAPTGPLLAPPAGATLNRLREPLLRRLSELLDQAPEGRGWRRLAELAGSRGRLRLS
  CLDLEQCSLKVLEPEGSPSLCLLKLMGEKGCTVTELSDFLQAMEHTEVLQLLSPPGIKITVNPESKAVLA
  GQFVKLCCRATGHPFVQYQWFKMNKEIPNGNTSELIFNAVHVKDAGFYVCRVNNNFTFEFSQWSQLDVCD
  IPESFQRSVDGVSESKLQICVEPTSQKLMPGSTLVLQCVAVGSPIPHYQWFKNELPLTHETKKLYMVPYV
  DLEHQGTYWCHVYNDRDSQDSKKVEIIIDELNNLGHPDNKEQTTDQPLAKDKVALLIGNMNYREHPKLKA
  PLVDVYELTNLLRQLDFKVVSLLDLTEYEMRNAVDEFLLLLDKGVYGLLYYAGHGYENFGNSFMVPVDAP
  NPYRSENCLCVQNILKLMQEKETGLNVFLLDMCRKRNDYDDTIPILDALKVTANIVFGYATCQGAEAFEI
  QHSGLANGIFMKFLKDRLLEDKKITVLLDEVAEDMGKCHLTKGKQALEIRSSLSEKRALTDPIQGTEYSA
  ESLVRNLQWAKAHELPESMCLKFDCGVQIQLGFAAEFSNVMIIYTSIVYKPPEIIMCDAYVTDFPLDLDI
  DPKDANKGTPEETGSYLVSKDLPKHCLYTRLSSLQKLKEHLVFTVCLSYQYSGLEDTVEDKQEVNVGKPL
  IAKLDMHRGLGRKTCFQTCLMSNGPYQSSAATSGGAGHYHSLQDPFHGVYHSHPGNPSNVTPADSCHCSR
  TPDAFISSFAHHASCHFSRSNVPVETTDEIPFSFSDRLRISEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173844
• ORF Size: 2439 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173844.1, NM_173844.2, NP_776216.1
• RefSeq Size: 4996
• RefSeq ORF: 2442
• Locus ID: 10892
• Protein Families: Druggable Genome, Protease
• Protein Pathways: B cell receptor signaling pathway, T cell receptor signaling pathway
• MW: 90.9 kDa
• Gene Summary: This gene encodes a caspase-like protease that plays a role in BCL10-induced activation of NF-kappaB. The protein is a component of the CARMA1-BCL10-MALT1 (CBM) signalosome that triggers NF-kappaB signaling and lymphoctye activation following antigen-receptor stimulation. Mutations in this gene result in immunodeficiency 12 (IMD12). This gene has been found to be recurrently rearranged in chromosomal translocations with other genes in mucosa-associated lymphoid tissue lymphomas, including a t(11;18)(q21;q21) translocation with the baculoviral IAP repeat-containing protein 3 (also known as apoptosis inhibitor 2) locus [BIRC3(API2)-MALT1], and a t(14;18)(q32;q21) translocation with the immunoglobulin heavy chain locus (IGH-MALT1). Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2018]