MRPS5 (NM_031902) Human Tagged ORF Clone

CAT#: RC204343

MRPS5 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein S5 (MRPS5), nuclear gene encoding mitochondrial protein

Product Images

Western blot validation of overexpression lysate (Cat# LY410449) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204343 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MRPS5
• Synonyms: MRP-S5; S5mt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204343 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCGCGGTGCGCGCTGTGGGCTGCCTCCCCGTGCTGTGTAGCGGGACGGCAGGTCATTTATTGG
  GGAGGCAGTGTTCCCTAAACACCTTACCAGCAGCTTCCATTTTGGCATGGAAGAGTGTTCTCGGCAATGG
  CCATTTGTCATCACTGGGAACCAGAGACACCCATCCCTACGCCAGCTTGAGCCGTGCACTGCAGACACAA
  TGCTGTATTTCTTCTCCCAGTCACCTGATGAGCCAGCAGTATAGACCATATAGTTTCTTCACTAAATTGA
  CTGCAGATGAGCTGTGGAAAGGCGCTTTAGCAGAGACTGGTGCTGGAGCAAAAAAAGGAAGAGGCAAAAG
  AACTAAAAAGAAGAAAAGAAAGGATCTGAACAGGGGTCAGATCATTGGTGAAGGGCGTTATGGTTTTCTA
  TGGCCCGGACTGAATGTCCCTCTTATGAAAAATGGAGCAGTGCAGACCATTGCCCAAAGAAGCAAGGAAG
  AGCAGGAGAAGGTGGAGGCAGACATGATCCAGCAGAGAGAAGAGTGGGACCGAAAGAAGAAGATGAAGGT
  TAAACGGGAGCGAGGATGGAGTGGAAACTCATGGGGAGGCATCAGTCTTGGCCCCCCTGACCCTGGTCCC
  TGTGGAGAAACATATGAGGATTTTGATACCAGGATACTTGAGGTAAGAAACGTTTTCACTATGACTGCGA
  AAGAGGGAAGAAAGAAATCGATCCGTGTCTTGGTGGCTGTGGGGAACGGAAAAGGAGCTGCAGGTTTTTC
  TATTGGGAAAGCTACTGATCGGATGGATGCTTTCAGGAAAGCAAAGAACAGAGCAGTTCACCATTTGCAT
  TATATAGAACGATATGAAGACCATACAATATTCCATGATATTTCATTAAGATTTAAAAGGACGCATATCA
  AGATGAAGAAACAACCCAAAGGTTACGGCCTCCGCTGCCACAGGGCCATCATCACCATCTGCCGGCTCAT
  TGGCATCAAAGACATGTATGCCAAGGTCTCTGGGTCCATTAATATGCTCAGCCTCACCCAGGGCCTCTTC
  CGTGGGCTCTCCAGACAGGAAACCCATCAACAGCTGGCTGATAAGAAGGGCCTCCATGTTGTGGAAATCC
  GGGAGGAATGTGGCCCTCTGCCCATTGTGGTTGCGTCCCCCCGGGGGCCCTTGAGGAAGGATCCAGAGCC
  AGAAGATGAGGTTCCAGACGTCAAACTGGACTGGGAAGATGTGAAGACTGCACAGGGAATGAAGCGCTCT
  GTGTGGTCTAATTTGAAGAGAGCCGCCACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204343 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATAVRAVGCLPVLCSGTAGHLLGRQCSLNTLPAASILAWKSVLGNGHLSSLGTRDTHPYASLSRALQTQ
  CCISSPSHLMSQQYRPYSFFTKLTADELWKGALAETGAGAKKGRGKRTKKKKRKDLNRGQIIGEGRYGFL
  WPGLNVPLMKNGAVQTIAQRSKEEQEKVEADMIQQREEWDRKKKMKVKRERGWSGNSWGGISLGPPDPGP
  CGETYEDFDTRILEVRNVFTMTAKEGRKKSIRVLVAVGNGKGAAGFSIGKATDRMDAFRKAKNRAVHHLH
  YIERYEDHTIFHDISLRFKRTHIKMKKQPKGYGLRCHRAIITICRLIGIKDMYAKVSGSINMLSLTQGLF
  RGLSRQETHQQLADKKGLHVVEIREECGPLPIVVASPRGPLRKDPEPEDEVPDVKLDWEDVKTAQGMKRS
  VWSNLKRAAT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031902
• ORF Size: 1290 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031902.1, NM_031902.2, NM_031902.3, NM_031902.4, NP_114108.1
• RefSeq Size: 1678 bp
• RefSeq ORF: 1293 bp
• Locus ID: 64969
• Domains: Ribosomal_S5, Ribosomal_S5_C
• MW: 48 kDa
• Gene Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S5P family. Pseudogenes corresponding to this gene are found on chromosomes 4q, 5q, and 18q. [provided by RefSeq, Jul 2008]