COQ6 (NM_182476) Human Tagged ORF Clone

CAT#: RC204353

COQ6 (Myc-DDK-tagged)-Human coenzyme Q6 homolog, monooxygenase (S. cerevisiae) (COQ6), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY405540) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204353 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: COQ6
• Synonyms: CGI-10; CGI10; COQ10D6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204353 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCGGCTTGTCAGCCGATGCGGGGCTGTGCGTGCAGCTCCCCACAGCGGCCCGCTGGTGTCCT
  GGCGCAGGTGGTCCGGCGCCTCAACAGACACCGTGTATGACGTGGTGGTGTCGGGTGGAGGCCTGGTGGG
  CGCTGCCATGGCCTGTGCCTTGGGATATGATATTCACTTTCATGACAAGAAAATCCTGTTGCTCGAAGCA
  GGTCCAAAGAAAGTACTGGAGAAATTGTCAGAAACTTACAGCAACAGGGTCAGCTCCATTTCCCCTGGCT
  CTGCAACGCTTCTCAGTAGTTTTGGTGCCTGGGACCATATCTGCAACATGAGATACAGAGCCTTTCGGCG
  AATGCAGGTGTGGGACGCCTGCTCAGAGGCCCTGATAATGTTTGATAAGGATAATTTAGATGACATGGGC
  TATATCGTGGAGAATGATGTCATCATGCATGCTCTCACTAAGCAGTTGGAGGCTGTGTCTGACCGAGTGA
  CGGTTCTCTACAGGAGCAAAGCCATTCGCTATACCTGGCCTTGTCCATTTCCTATGGCCGACTCCAGCCC
  TTGGGTTCATATTACCCTAGGTGATGGCAGCACCTTCCAGACCAAATTGTTGATAGGTGCAGATGGTCAC
  AACTCCGGAGTACGGCAGGCTGTTGGAATCCAGAATGTGAGCTGGAACTATGACCAGTCTGCTGTTGTGG
  CTACTCTGCATTTATCAGAGGCCACAGAAAACAACGTAGCCTGGCAGAGATTTCTTCCCTCTGGGCCTAT
  TGCTCTGCTCCCGCTCTCAGACACCTTGAGTTCCTTGGTTTGGTCCACGTCCCATGAACATGCAGCAGAG
  CTAGTTAGCATGGATGAGGAAAAATTTGTGGATGCCGTTAACTCTGCCTTTTGGAGTGATGCTGACCACA
  CGGACTTCATCGACACAGCTGGTGCCATGCTGCAGTATGCTGTCAGCCTTCTGAAGCCCACTAAGGTCTC
  GGCTCGCCAGCTGCCCCCAAGCGTAGCCAGGGTGGATGCCAAAAGCCGAGTTCTGTTTCCTCTTGGGTTG
  GGACATGCTGCTGAGTACGTCAGGCCTCGGGTGGCGCTCATTGGGGATGCAGCCCACAGAGTCCATCCGC
  TTGCAGGACAGGGTGTCAACATGGGCTTTGGGGATATCTCCAGCTTGGCCCATCACCTCAGTACGGCAGC
  CTTCAATGGGAAGGACTTAGGTTCCGTGAGCCACCTCACAGGTTATGAAACAGAAAGACAGCGTCACAAC
  ACTGCTCTTCTGGCTGCTACAGACTTACTAAAAAGGCTCTATTCTACCAGTGCCTCCCCGCTTGTGTTGC
  TCAGGACGTGGGGCTTGCAGGCCACAAATGCAGTGTCTCCACTCAAAGAACAGATTATGGCCTTTGCAAG
  CAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204353 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAARLVSRCGAVRAAPHSGPLVSWRRWSGASTDTVYDVVVSGGGLVGAAMACALGYDIHFHDKKILLLEA
  GPKKVLEKLSETYSNRVSSISPGSATLLSSFGAWDHICNMRYRAFRRMQVWDACSEALIMFDKDNLDDMG
  YIVENDVIMHALTKQLEAVSDRVTVLYRSKAIRYTWPCPFPMADSSPWVHITLGDGSTFQTKLLIGADGH
  NSGVRQAVGIQNVSWNYDQSAVVATLHLSEATENNVAWQRFLPSGPIALLPLSDTLSSLVWSTSHEHAAE
  LVSMDEEKFVDAVNSAFWSDADHTDFIDTAGAMLQYAVSLLKPTKVSARQLPPSVARVDAKSRVLFPLGL
  GHAAEYVRPRVALIGDAAHRVHPLAGQGVNMGFGDISSLAHHLSTAAFNGKDLGSVSHLTGYETERQRHN
  TALLAATDLLKRLYSTSASPLVLLRTWGLQATNAVSPLKEQIMAFASK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182476
• ORF Size: 1404 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182476.1, NM_182476.2, NP_872282.1
• RefSeq Size: 1615 bp
• RefSeq ORF: 1407 bp
• Locus ID: 51004
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Ubiquinone and other terpenoid-quinone biosynthesis
• MW: 50.9 kDa
• Gene Summary: The protein encoded by this gene belongs to the ubiH/COQ6 family. It is an evolutionarily conserved monooxygenase required for the biosynthesis of coenzyme Q10 (or ubiquinone), which is an essential component of the mitochondrial electron transport chain, and one of the most potent lipophilic antioxidants implicated in the protection of cell damage by reactive oxygen species. Knockdown of this gene in mouse and zebrafish results in decreased growth due to increased apoptosis. Mutations in this gene are associated with autosomal recessive coenzyme Q10 deficiency-6 (COQ10D6), which manifests as nephrotic syndrome with sensorineural deafness. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jun 2012]