PTOP (ACD) (NM_001082486) Human Tagged ORF Clone

CAT#: RC204381

ACD (Myc-DDK-tagged)-Human adrenocortical dysplasia homolog (mouse) (ACD), transcript variant 1


  "NM_001082486" in other vectors (6)

Reconstitution Protocol

USD 420.00

In Stock*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ACD
Synonyms PIP1; PTOP; TINT1; TPP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC204381 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTGGCCGCTGTCAGAGTGACGCCGCGATGAGAGTAAACGGGCCAGCATCCCGTGCACCAGCGGGGT
GGACCAGCGGGAGTCTGCACACAGGCCCCCGAGCAGGACGCCCTCGTGCGCAGGCGCGGGGTGTACGTGG
GCGGGGCCTCCTCCTCCGGCCCCGCCCAGCGAAGGAGCTTCCTCTTCCGAGGAAAGGCGGGGCCTGGGCG
CCCGCGGGAAACCCGGGCCCGTTGCATCCGCTGGGTGTAGCCGTGGGGATGGCAGGTTCGGGGAGGCTGG
TCCTACGGCCCTGGATTCGGGAGCTGATTCTGGGGTCAGAGACACCCTCCAGTCCACGAGCCGGGCAGCT
GCTTGAGGTACTACAGGACGCCGAGGCCGCGGTCGCGGGCCCATCCCACGCCCCTGATACGTCCGACGTC
GGGGCCACGCTGCTTGTGTCTGACGGGACCCACAGTGTCCGATGCCTGGTGACGCGGGAGGCCCTGGACA
CCTCGGACTGGGAGGAGAAGGAGTTCGGCTTCCGCGGGACAGAGGGCCGGCTGCTGCTGCTGCAGGACTG
CGGGGTTCATGTCCAGGTCGCTGAGGGCGGCGCGCCCGCAGAGTTCTATCTCCAGGTGGACCGCTTCAGC
CTGCTGCCCACGGAGCAGCCCCGGCTACGGGTGCCTGGTTGCAACCAAGACTTAGATGTTCAGAAAAAGC
TCTATGACTGCCTTGAGGAGCACCTTTCAGAGTCCACCTCGTCCAATGCAGGCCTATCACTGTCCCAGCT
TCTGGATGAAATGCGGGAGGACCAGGAGCATCAGGGGGCACTCGTGTGCCTGGCTGAAAGCTGCCTGACA
CTGGAGGGCCCTTGCACAGCACCCCCTGTCACCCACTGGGCTGCCTCACGATGCAAGGCCACGGGAGAAG
CTGTGTACACTGTCCCCAGCTCAATGCTGTGCATCTCTGAGAATGACCAGCTAATTCTGAGCTCTCTAGG
CCCCTGTCAGAGGACACAGGGCCCTGAGCTGCCCCCACCAGACCCGGCTCTGCAGGACCTATCTCTGACC
CTCATAGCCTCTCCTCCTTCCTCACCCAGTTCCTCAGGAACCCCGGCCTTACCCGGCCACATGTCATCCG
AGGAAAGTGGTACCAGCATCAGCCTTCTGCCTGCCCTGTCCTTGGCTGCTCCAGACCCAGGGCAGAGAAG
CAGCTCCCAGCCCTCACCAGCCATCTGCTCAGCCCCTGCCACCCTGACCCCCAGGTCCCCACACGCCAGC
CGTACCCCCAGCTCCCCACTCCAGAGCTGCACTCCCAGTCTCTCACCCCGTAGCCATGTCCCCAGTCCAC
ACCAGGCTCTTGTGACCAGGCCCCAGAAACCTAGCCTGGAGTTCAAGGAGTTTGTAGGGTTGCCCTGCAA
GAATCGGCCGCCTTTTCCCAGGACCGGAGCTACCAGGGGAGCCCAGGAGCCCTGCTCTGTCTGGGAACCC
CCAAAGAGGCATCGTGATGGTTCTGCCTTCCAGTATGAGTATGAGCCACCCTGCACGTCCCTCTGTGCTC
GGGTCCAAGCTGCCAGGCTTCCTCCCCAGCTCATGGCCTGGGCCTTGCACTTTCTGATGGATGCACAGCC
AGGGTCTGAGCCAACTCCGATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC204381 protein sequence
Red=Cloning site Green=Tags(s)

MPGRCQSDAAMRVNGPASRAPAGWTSGSLHTGPRAGRPRAQARGVRGRGLLLRPRPAKELPLPRKGGAWA
PAGNPGPLHPLGVAVGMAGSGRLVLRPWIRELILGSETPSSPRAGQLLEVLQDAEAAVAGPSHAPDTSDV
GATLLVSDGTHSVRCLVTREALDTSDWEEKEFGFRGTEGRLLLLQDCGVHVQVAEGGAPAEFYLQVDRFS
LLPTEQPRLRVPGCNQDLDVQKKLYDCLEEHLSESTSSNAGLSLSQLLDEMREDQEHQGALVCLAESCLT
LEGPCTAPPVTHWAASRCKATGEAVYTVPSSMLCISENDQLILSSLGPCQRTQGPELPPPDPALQDLSLT
LIASPPSSPSSSGTPALPGHMSSEESGTSISLLPALSLAAPDPGQRSSSQPSPAICSAPATLTPRSPHAS
RTPSSPLQSCTPSLSPRSHVPSPHQALVTRPQKPSLEFKEFVGLPCKNRPPFPRTGATRGAQEPCSVWEP
PKRHRDGSAFQYEYEPPCTSLCARVQAARLPPQLMAWALHFLMDAQPGSEPTPM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001082486
ORF Size 1632 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001082486.1, NP_001075955.1
RefSeq Size 2095 bp
RefSeq ORF 1377 bp
Locus ID 65057
MW 57.7 kDa
Gene Summary This gene encodes a protein that is involved in telomere function. This protein is one of six core proteins in the telosome/shelterin telomeric complex, which functions to maintain telomere length and to protect telomere ends. Through its interaction with other components, this protein plays a key role in the assembly and stabilization of this complex, and it mediates the access of telomerase to the telomere. Multiple transcript variants encoding different isoforms have been found for this gene. This gene, which is also referred to as TPP1, is distinct from the unrelated TPP1 gene on chromosome 11, which encodes tripeptidyl-peptidase I. [provided by RefSeq, Jul 2008]

Other Versions

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.