ICT1 (MRPL58) (NM_001545) Human Tagged ORF Clone

CAT#: RC204448

ICT1 (Myc-DDK-tagged)-Human immature colon carcinoma transcript 1 (ICT1)

Product Images

Western blot validation of overexpression lysate (Cat# LY419875) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204448 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified MRPL58 protein (Cat# TP304448). The protein was produced from HEK293T cells transfected with MRPL58 cDNA clone (Cat# RC204448) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MRPL58
• Synonyms: DS-1; DS1; ICT1; MRP-L58
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204448 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCACCAGGTGCCTGCGCTGGGGCCTGAGCCGAGCCGGAGTCTGGCTGCTCCCACCGCCCGCAC
  GGTGCCCACGCCGGGCGCTGCACAAGCAGAAAGACGGCACTGAGTTCAAGAGCATCTACAGCCTGGACAA
  GCTCTACCCCGAATCTCAGGGCTCGGACACCGCCTGGAGGGTCCCGAATGGTGCAAAGCAAGCCGACAGT
  GACATCCCTCTAGATCGCTTGACAATATCTTATTGTCGGAGTAGTGGTCCTGGGGGGCAGAATGTGAACA
  AAGTGAATTCCAAGGCAGAAGTCAGGTTCCATTTGGCAACTGCCGAGTGGATCGCGGAGCCCGTGCGGCA
  GAAGATAGCCATCACGCATAAAAACAAGATCAACAGGTTAGGAGAGTTGATCCTCACCTCTGAGAGCAGC
  CGCTATCAGTTCCGGAATCTGGCAGATTGCCTGCAGAAAATTCGAGACATGATCACTGAGGCCAGCCAGA
  CACCGAAGGAGCCAACAAAAGAAGATGTTAAACTTCATAGAATCAGGATAGAAAACATGAATCGGGAAAG
  GCTGAGACAAAAGAGAATTCATTCTGCTGTAAAGACAAGCAGGAGGGTCGACATGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204448 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAATRCLRWGLSRAGVWLLPPPARCPRRALHKQKDGTEFKSIYSLDKLYPESQGSDTAWRVPNGAKQADS
  DIPLDRLTISYCRSSGPGGQNVNKVNSKAEVRFHLATAEWIAEPVRQKIAITHKNKINRLGELILTSESS
  RYQFRNLADCLQKIRDMITEASQTPKEPTKEDVKLHRIRIENMNRERLRQKRIHSAVKTSRRVDMD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001545
• ORF Size: 618 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001545.1, NM_001545.2, NP_001536.1
• RefSeq Size: 888 bp
• RefSeq ORF: 621 bp
• Locus ID: 3396
• Cytogenetics: 17q25.1
• MW: 23.6 kDa
• Gene Summary: 'The protein encoded by this gene is a peptidyl-tRNA hydrolase and a vital component of the large mitochondrial ribosome. The encoded protein serves as a ribosome release factor for this ribosome, which translates mitochondrial genes. This protein may be responsible for degrading prematurely-terminated polypeptides and for reusing stalled ribosomes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014]'