FRK (NM_002031) Human Tagged ORF Clone

CAT#: RC204460

FRK (Myc-DDK-tagged)-Human fyn-related kinase (FRK)

Product Images

Western blot validation of overexpression lysate (Cat# LY400740) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204460 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FRK
• Synonyms: GTK; PTK5; RAK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204460 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAACATCTGTCAGAGGCTCTGGGAGTACCTAGAACCCTATCTCCCCTGTTTGTCCACGGAGGCAG
  ACAAGTCAACCGTGATTGAAAATCCAGGGGCCCTTTGCTCTCCCCAGTCACAGAGGCATGGCCACTACTT
  TGTGGCTTTGTTTGATTACCAGGCTCGGACTGCTGAGGACTTGAGCTTCCGAGCAGGTGACAAACTTCAA
  GTTCTGGACACTTTGCATGAGGGCTGGTGGTTTGCCAGACACTTGGAGAAAAGACGAGATGGCTCCAGTC
  AGCAACTACAAGGCTATATTCCTTCTAACTACGTGGCTGAGGACAGAAGCCTACAGGCAGAGCCGTGGTT
  CTTTGGAGCAATCGGAAGATCAGATGCAGAGAAACAACTATTATATTCAGAAAACAAGACCGGTTCCTTT
  CTAATCAGAGAAAGTGAAAGCCAAAAAGGAGAATTCTCTCTTTCAGTTTTAGATGGAGCAGTTGTAAAAC
  ACTACAGAATTAAAAGACTGGATGAAGGGGGATTTTTTCTCACGCGAAGAAGAATCTTTTCAACACTGAA
  CGAATTTGTGAGCCACTACACCAAGACAAGTGACGGCCTGTGTGTCAAGCTGGGGAAACCATGCTTAAAG
  ATCCAGGTCCCAGCTCCATTTGATTTGTCGTATAAAACCGTGGACCAATGGGAGATAGACCGCAACTCCA
  TACAGCTTCTGAAGCGATTGGGATCTGGTCAGTTTGGCGAAGTATGGGAAGGTCTGTGGAACAATACCAC
  TCCAGTAGCAGTGAAAACATTAAAACCAGGTTCAATGGATCCAAATGACTTCCTGAGGGAGGCACAGATA
  ATGAAGAACCTAAGACATCCAAAGCTTATCCAGCTTTATGCTGTTTGCACTTTAGAAGATCCAATTTATA
  TTATTACAGAGTTGATGAGACATGGAAGTCTGCAAGAATATCTCCAAAATGACACTGGATCAAAAATCCA
  TCTGACTCAACAGGTAGACATGGCGGCACAGGTTGCCTCTGGAATGGCCTATCTGGAGTCTCGGAACTAC
  ATTCACAGAGATCTGGCTGCCAGAAATGTCCTCGTTGGTGAACATAATATCTACAAAGTAGCAGATTTTG
  GACTTGCCAGAGTTTTTAAGGTAGATAATGAAGACATCTATGAATCTAGACACGAAATAAAGCTGCCGGT
  GAAGTGGACTGCGCCCGAAGCCATTCGTAGTAATAAATTCAGCATTAAGTCCGATGTATGGTCATTTGGA
  ATCCTTCTTTATGAAATCATTACTTATGGCAAAATGCCTTACAGTGGTATGACAGGTGCCCAGGTAATCC
  AGATGTTGGCTCAAAACTATAGACTTCCGCAACCATCCAACTGTCCACAGCAATTTTACAACATCATGTT
  GGAGTGCTGGAATGCAGAGCCTAAGGAACGACCTACATTTGAGACACTGCGTTGGAAACTTGAAGACTAT
  TTTGAAACAGACTCTTCATATTCAGATGCAAATAACTTCATAAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204460 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSNICQRLWEYLEPYLPCLSTEADKSTVIENPGALCSPQSQRHGHYFVALFDYQARTAEDLSFRAGDKLQ
  VLDTLHEGWWFARHLEKRRDGSSQQLQGYIPSNYVAEDRSLQAEPWFFGAIGRSDAEKQLLYSENKTGSF
  LIRESESQKGEFSLSVLDGAVVKHYRIKRLDEGGFFLTRRRIFSTLNEFVSHYTKTSDGLCVKLGKPCLK
  IQVPAPFDLSYKTVDQWEIDRNSIQLLKRLGSGQFGEVWEGLWNNTTPVAVKTLKPGSMDPNDFLREAQI
  MKNLRHPKLIQLYAVCTLEDPIYIITELMRHGSLQEYLQNDTGSKIHLTQQVDMAAQVASGMAYLESRNY
  IHRDLAARNVLVGEHNIYKVADFGLARVFKVDNEDIYESRHEIKLPVKWTAPEAIRSNKFSIKSDVWSFG
  ILLYEIITYGKMPYSGMTGAQVIQMLAQNYRLPQPSNCPQQFYNIMLECWNAEPKERPTFETLRWKLEDY
  FETDSSYSDANNFIR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002031
• ORF Size: 1515 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002031.1, NM_002031.2, NP_002022.1
• RefSeq Size: 2864 bp
• RefSeq ORF: 1518 bp
• Locus ID: 2444
• Domains: pkinase, SH2, TyrKc, SH3, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• MW: 58.3 kDa
• Gene Summary: The protein encoded by this gene belongs to the TYR family of protein kinases. This tyrosine kinase is a nuclear protein and may function during G1 and S phase of the cell cycle and suppress growth. [provided by RefSeq, Jul 2008]