FRK (NM_002031) Human Tagged ORF Clone
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | FRK |
Synonyms | GTK; PTK5; RAK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC204460 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAACATCTGTCAGAGGCTCTGGGAGTACCTAGAACCCTATCTCCCCTGTTTGTCCACGGAGGCAG ACAAGTCAACCGTGATTGAAAATCCAGGGGCCCTTTGCTCTCCCCAGTCACAGAGGCATGGCCACTACTT TGTGGCTTTGTTTGATTACCAGGCTCGGACTGCTGAGGACTTGAGCTTCCGAGCAGGTGACAAACTTCAA GTTCTGGACACTTTGCATGAGGGCTGGTGGTTTGCCAGACACTTGGAGAAAAGACGAGATGGCTCCAGTC AGCAACTACAAGGCTATATTCCTTCTAACTACGTGGCTGAGGACAGAAGCCTACAGGCAGAGCCGTGGTT CTTTGGAGCAATCGGAAGATCAGATGCAGAGAAACAACTATTATATTCAGAAAACAAGACCGGTTCCTTT CTAATCAGAGAAAGTGAAAGCCAAAAAGGAGAATTCTCTCTTTCAGTTTTAGATGGAGCAGTTGTAAAAC ACTACAGAATTAAAAGACTGGATGAAGGGGGATTTTTTCTCACGCGAAGAAGAATCTTTTCAACACTGAA CGAATTTGTGAGCCACTACACCAAGACAAGTGACGGCCTGTGTGTCAAGCTGGGGAAACCATGCTTAAAG ATCCAGGTCCCAGCTCCATTTGATTTGTCGTATAAAACCGTGGACCAATGGGAGATAGACCGCAACTCCA TACAGCTTCTGAAGCGATTGGGATCTGGTCAGTTTGGCGAAGTATGGGAAGGTCTGTGGAACAATACCAC TCCAGTAGCAGTGAAAACATTAAAACCAGGTTCAATGGATCCAAATGACTTCCTGAGGGAGGCACAGATA ATGAAGAACCTAAGACATCCAAAGCTTATCCAGCTTTATGCTGTTTGCACTTTAGAAGATCCAATTTATA TTATTACAGAGTTGATGAGACATGGAAGTCTGCAAGAATATCTCCAAAATGACACTGGATCAAAAATCCA TCTGACTCAACAGGTAGACATGGCGGCACAGGTTGCCTCTGGAATGGCCTATCTGGAGTCTCGGAACTAC ATTCACAGAGATCTGGCTGCCAGAAATGTCCTCGTTGGTGAACATAATATCTACAAAGTAGCAGATTTTG GACTTGCCAGAGTTTTTAAGGTAGATAATGAAGACATCTATGAATCTAGACACGAAATAAAGCTGCCGGT GAAGTGGACTGCGCCCGAAGCCATTCGTAGTAATAAATTCAGCATTAAGTCCGATGTATGGTCATTTGGA ATCCTTCTTTATGAAATCATTACTTATGGCAAAATGCCTTACAGTGGTATGACAGGTGCCCAGGTAATCC AGATGTTGGCTCAAAACTATAGACTTCCGCAACCATCCAACTGTCCACAGCAATTTTACAACATCATGTT GGAGTGCTGGAATGCAGAGCCTAAGGAACGACCTACATTTGAGACACTGCGTTGGAAACTTGAAGACTAT TTTGAAACAGACTCTTCATATTCAGATGCAAATAACTTCATAAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204460 protein sequence
Red=Cloning site Green=Tags(s) MSNICQRLWEYLEPYLPCLSTEADKSTVIENPGALCSPQSQRHGHYFVALFDYQARTAEDLSFRAGDKLQ VLDTLHEGWWFARHLEKRRDGSSQQLQGYIPSNYVAEDRSLQAEPWFFGAIGRSDAEKQLLYSENKTGSF LIRESESQKGEFSLSVLDGAVVKHYRIKRLDEGGFFLTRRRIFSTLNEFVSHYTKTSDGLCVKLGKPCLK IQVPAPFDLSYKTVDQWEIDRNSIQLLKRLGSGQFGEVWEGLWNNTTPVAVKTLKPGSMDPNDFLREAQI MKNLRHPKLIQLYAVCTLEDPIYIITELMRHGSLQEYLQNDTGSKIHLTQQVDMAAQVASGMAYLESRNY IHRDLAARNVLVGEHNIYKVADFGLARVFKVDNEDIYESRHEIKLPVKWTAPEAIRSNKFSIKSDVWSFG ILLYEIITYGKMPYSGMTGAQVIQMLAQNYRLPQPSNCPQQFYNIMLECWNAEPKERPTFETLRWKLEDY FETDSSYSDANNFIR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002031 |
ORF Size | 1515 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002031.1, NM_002031.2, NP_002022.1 |
RefSeq Size | 2864 bp |
RefSeq ORF | 1518 bp |
Locus ID | 2444 |
Domains | pkinase, SH2, TyrKc, SH3, S_TKc |
Protein Families | Druggable Genome, Protein Kinase |
MW | 58.3 kDa |
Gene Summary | The protein encoded by this gene belongs to the TYR family of protein kinases. This tyrosine kinase is a nuclear protein and may function during G1 and S phase of the cell cycle and suppress growth. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC127842 | FRK (untagged)-Human fyn-related kinase (FRK) |
USD 760.00 |
|
RG204460 | FRK (GFP-tagged) - Human fyn-related kinase (FRK) |
USD 460.00 |
|
RC204460L1 | Lenti ORF clone of Human fyn-related kinase (FRK), Myc-DDK-tagged |
USD 768.00 |
|
RC204460L2 | Lenti ORF clone of Human fyn-related kinase (FRK), mGFP tagged |
USD 620.00 |
|
RC204460L3 | Lenti ORF clone of Human fyn-related kinase (FRK), Myc-DDK-tagged |
USD 620.00 |
|
RC204460L4 | Lenti ORF clone of Human fyn-related kinase (FRK), mGFP tagged |
USD 620.00 |
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