FBP1 (NM_000507) Human Tagged ORF Clone
CAT#: RC204461
FBP1 (Myc-DDK-tagged)-Human fructose-1,6-bisphosphatase 1 (FBP1), transcript variant 1
"NM_000507" in other vectors (7)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | FBP1 |
Synonyms | FBP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC204461 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGACCAGGCGCCCTTCGACACGGACGTCAACACCCTGACCCGCTTCGTCATGGAGGAGGGCAGGA AGGCCCGCGGCACGGGCGAGTTGACCCAGCTGCTCAACTCGCTCTGCACAGCAGTCAAAGCCATCTCTTC GGCGGTGCGCAAGGCGGGCATCGCGCACCTCTATGGCATTGCTGGTTCTACCAACGTGACAGGTGATCAA GTTAAGAAGCTGGACGTCCTCTCCAACGACCTGGTTATGAACATGTTAAAGTCATCCTTTGCCACGTGTG TTCTCGTGTCAGAAGAAGATAAACACGCCATCATAGTGGAACCGGAGAAAAGGGGTAAATATGTGGTCTG TTTTGATCCCCTTGATGGATCTTCCAACATCGATTGCCTTGTGTCCGTTGGAACCATTTTTGGCATCTAT AGAAAGAAATCAACTGATGAGCCTTCTGAGAAGGATGCTCTGCAACCAGGCCGGAACCTGGTGGCAGCCG GCTACGCACTGTATGGCAGTGCCACCATGCTGGTCCTTGCCATGGACTGTGGGGTCAACTGCTTCATGCT GGACCCGGCCATCGGGGAGTTCATTTTGGTGGACAAGGATGTGAAGATAAAAAAGAAAGGTAAAATCTAC AGCCTTAACGAGGGCTACGCCAGGGACTTTGACCCTGCCGTCACTGAGTACATCCAGAGGAAGAAGTTCC CCCCAGATAATTCAGCTCCTTATGGGGCCCGGTATGTGGGCTCCATGGTGGCTGATGTTCATCGCACTCT GGTCTACGGAGGGATATTTCTGTACCCCGCTAACAAGAAGAGCCCCAATGGAAAGCTGAGACTGCTGTAC GAATGCAACCCCATGGCCTACGTCATGGAGAAGGCTGGGGGAATGGCCACCACTGGGAAGGAGGCCGTGT TAGACGTCATTCCCACAGACATTCACCAGAGGGCGCCGGTGATCTTGGGATCCCCCGACGACGTGCTCGA GTTCCTGAAGGTGTATGAGAAGCACTCTGCCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204461 protein sequence
Red=Cloning site Green=Tags(s) MADQAPFDTDVNTLTRFVMEEGRKARGTGELTQLLNSLCTAVKAISSAVRKAGIAHLYGIAGSTNVTGDQ VKKLDVLSNDLVMNMLKSSFATCVLVSEEDKHAIIVEPEKRGKYVVCFDPLDGSSNIDCLVSVGTIFGIY RKKSTDEPSEKDALQPGRNLVAAGYALYGSATMLVLAMDCGVNCFMLDPAIGEFILVDKDVKIKKKGKIY SLNEGYARDFDPAVTEYIQRKKFPPDNSAPYGARYVGSMVADVHRTLVYGGIFLYPANKKSPNGKLRLLY ECNPMAYVMEKAGGMATTGKEAVLDVIPTDIHQRAPVILGSPDDVLEFLKVYEKHSAQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_000507 |
ORF Size | 1014 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000507.1, NM_000507.2, NM_000507.3, NP_000498.2 |
RefSeq Size | 1647 bp |
RefSeq ORF | 1017 bp |
Locus ID | 2203 |
Domains | FBPase |
Protein Families | Druggable Genome, Stem cell - Pluripotency |
Protein Pathways | Fructose and mannose metabolism, Glycolysis / Gluconeogenesis, Insulin signaling pathway, Metabolic pathways, Pentose phosphate pathway |
MW | 36.8 kDa |
Gene Summary | Fructose-1,6-bisphosphatase 1, a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1,6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC119828 | FBP1 (untagged)-Human fructose-1,6-bisphosphatase 1 (FBP1), transcript variant 1 |
USD 310.00 |
|
SC322529 | FBP1 (untagged)-Human fructose-1,6-bisphosphatase 1 (FBP1), transcript variant 1 |
USD 310.00 |
|
RG204461 | FBP1 (GFP-tagged) - Human fructose-1,6-bisphosphatase 1 (FBP1), transcript variant 1 |
USD 460.00 |
|
RC204461L1 | Lenti ORF clone of Human fructose-1,6-bisphosphatase 1 (FBP1), transcript variant 1, Myc-DDK-tagged |
USD 768.00 |
|
RC204461L2 | Lenti ORF clone of Human fructose-1,6-bisphosphatase 1 (FBP1), transcript variant 1, mGFP tagged |
USD 620.00 |
|
RC204461L3 | Lenti ORF clone of Human fructose-1,6-bisphosphatase 1 (FBP1), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC204461L4 | Lenti ORF clone of Human fructose-1,6-bisphosphatase 1 (FBP1), transcript variant 1, mGFP tagged |
USD 620.00 |
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