CTRP6 (C1QTNF6) (NM_031910) Human Tagged ORF Clone

CAT#: RC204524

C1QTNF6 (Myc-DDK-tagged)-Human C1q and tumor necrosis factor related protein 6 (C1QTNF6), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY403130) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204524 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified C1QTNF6 protein (Cat# TP304524). The protein was produced from HEK293T cells transfected with C1QTNF6 cDNA clone (Cat# RC204524) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C1QTNF6
• Synonyms: CTFP6; CTRP6; ZACRP6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204524 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTGGCTCAGGGTCCGTGAGTCGCCTGGGGAGGCCACAGGACACAGGGTCACCATGGTGACAGCCG
  CCCTGGGTCCCGTCTGGGCAGCGCTCCTGCTCTTTCTCCTGATGTGTGAGATCCCTATGGTGGAGCTCAC
  CTTTGACAGAGCTGTGGCCAGCGGCTGCCAACGGTGCTGTGACTCTGAGGACCCCCTGGATCCTGCCCAT
  GTATCCTCAGCCTCTTCCTCCGGCCGCCCCCACGCCCTGCCTGAGATCAGACCCTACATTAATATCACCA
  TCCTGAAGGGTGACAAAGGGGACCCAGGCCCAATGGGCCTGCCAGGGTACATGGGCAGGGAGGGTCCCCA
  AGGGGAGCCTGGCCCTCAGGGCAGCAAGGGTGACAAGGGGGAGATGGGCAGCCCCGGCGCCCCGTGCCAG
  AAGCGCTTCTTCGCCTTCTCAGTGGGCCGCAAGACGGCCCTGCACAGCGGCGAGGACTTCCAGACACTGC
  TCTTCGAAAGGGTCTTTGTGAACCTTGATGGGTGCTTTGACATGGCGACCGGCCAGTTTGCTGCTCCCCT
  GCGTGGCATCTACTTCTTCAGCCTCAATGTGCACAGCTGGAATTACAAGGAGACGTACGTGCACATTATG
  CATAACCAGAAAGAGGCTGTCATCCTGTACGCGCAGCCCAGCGAGCGCAGCATCATGCAGAGCCAGAGTG
  TGATGCTGGACCTGGCCTACGGGGACCGCGTCTGGGTGCGGCTCTTCAAGCGCCAGCGCGAGAACGCCAT
  CTACAGCAACGACTTCGACACCTACATCACCTTCAGCGGCCACCTCATCAAGGCCGAGGACGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204524 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQWLRVRESPGEATGHRVTMVTAALGPVWAALLLFLLMCEIPMVELTFDRAVASGCQRCCDSEDPLDPAH
  VSSASSSGRPHALPEIRPYINITILKGDKGDPGPMGLPGYMGREGPQGEPGPQGSKGDKGEMGSPGAPCQ
  KRFFAFSVGRKTALHSGEDFQTLLFERVFVNLDGCFDMATGQFAAPLRGIYFFSLNVHSWNYKETYVHIM
  HNQKEAVILYAQPSERSIMQSQSVMLDLAYGDRVWVRLFKRQRENAIYSNDFDTYITFSGHLIKAEDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031910
• ORF Size: 834 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031910.1, NM_031910.2, NM_031910.3, NP_114116.3
• RefSeq Size: 2954 bp
• RefSeq ORF: 837 bp
• Locus ID: 114904
• Domains: C1Q
• Protein Families: Secreted Protein, Transmembrane
• MW: 30.9 kDa