ITPK1 (NM_014216) Human Tagged ORF Clone

CAT#: RC204537

ITPK1 (Myc-DDK-tagged)-Human inositol-tetrakisphosphate 1-kinase (ITPK1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY428194) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227205 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ITPK1 protein (Cat# TP304537). The protein was produced from HEK293T cells transfected with ITPK1 cDNA clone (Cat# RC204537) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ITPK1
• Synonyms: ITRPK1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204537 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGACCTTTCTGAAAGGGAAGAGAGTTGGCTACTGGCTGAGCGAGAAGAAAATCAAGAAGCTGAATT
  TCCAGGCCTTCGCCGAGCTGTGCAGGAAGCGAGGGATGGAGGTTGTGCAGCTGAACCTTAGCCGGCCGAT
  CGAGGAGCAGGGCCCCCTGGACGTCATCATCCACAAGCTGACTGACGTCATCCTTGAAGCCGACCAGAAT
  GATAGCCAGTCCCTGGAGCTGGTGCACAGGTTCCAGGAGTACATCGATGCCCACCCTGAGACCATCGTCC
  TGGACCCGCTCCCTGCCATCAGAACCCTGCTTGACCGCTCCAAGTCCTATGAGCTCATCCGGAAGATTGA
  GGCCTACATGGAAGACGACAGGATCTGCTCGCCACCCTTCATGGAGCTCACGAGCCTGTGCGGGGATGAC
  ACCATGCGGCTGCTGGAGAAGAACGGCTTGACTTTCCCATTCATTTGCAAAACCAGAGTGGCTCATGGCA
  CCAACTCTCACGAGATGGCTATCGTGTTCAACCAGGAGGGCCTGAACGCCATCCAGCCACCCTGCGTGGT
  CCAGAATTTCATCAACCACAACGCCGTCCTGTACAAGGTGTTCGTGGTTGGCGAGTCCTACACCGTGGTC
  CAGAGGCCCTCACTCAAGAACTTCTCCGCAGGCACATCAGACCGTGAGTCCATCTTCTTCAACAGCCACA
  ACGTGTCAAAGCCGGAGTCGTCATCGGTCCTGACGGAGCTGGACAAGATCGAGGGCGTGTTCGAGCGGCC
  GAGCGACGAGGTCATCCGGGAGCTCTCCCGGGCCCTGCGGCAGGCACTGGGCGTGTCACTCTTCGGCATC
  GACATCATCATCAACAACCAGACAGGGCAGCACGCCGTCATTGACATCAATGCCTTCCCAGGCTACGAGG
  GCGTGAGCGAGTTCTTCACAGACCTCCTGAACCACATCGCCACTGTCCTGCAGGGCCAGAGCACAGCCAT
  GGCAGCCACAGGGGACGTGGCCCTGCTGAGGCACAGCAAGCTTCTGGCCGAGCCGGCGGGCGGCCTGGTG
  GGCGAGCGGACATGCAGCGCCAGCCCCGGCTGCTGCGGCAGCATGATGGGCCAGGACGCGCCCTGGAAGG
  CTGAGGCCGACGCGGGCGGCACCGCCAAGCTGCCGCACCAGAGACTCGGCTGCAACGCCGGCGTGTCGCC
  CAGCTTCCAGCAGCATTGTGTGGCCTCCCTGGCCACCAAGGCCTCCTCCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204537 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQTFLKGKRVGYWLSEKKIKKLNFQAFAELCRKRGMEVVQLNLSRPIEEQGPLDVIIHKLTDVILEADQN
  DSQSLELVHRFQEYIDAHPETIVLDPLPAIRTLLDRSKSYELIRKIEAYMEDDRICSPPFMELTSLCGDD
  TMRLLEKNGLTFPFICKTRVAHGTNSHEMAIVFNQEGLNAIQPPCVVQNFINHNAVLYKVFVVGESYTVV
  QRPSLKNFSAGTSDRESIFFNSHNVSKPESSSVLTELDKIEGVFERPSDEVIRELSRALRQALGVSLFGI
  DIIINNQTGQHAVIDINAFPGYEGVSEFFTDLLNHIATVLQGQSTAMAATGDVALLRHSKLLAEPAGGLV
  GERTCSASPGCCGSMMGQDAPWKAEADAGGTAKLPHQRLGCNAGVSPSFQQHCVASLATKASSQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014216
• ORF Size: 1242 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014216.1, NM_014216.2, NM_014216.3, NM_014216.4, NM_014216.5, NP_055031.2
• RefSeq Size: 3385 bp
• RefSeq ORF: 1245 bp
• Locus ID: 3705
• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• MW: 45.6 kDa
• Gene Summary: This gene encodes an enzyme that belongs to the inositol 1,3,4-trisphosphate 5/6-kinase family. This enzyme regulates the synthesis of inositol tetraphosphate, and downstream products, inositol pentakisphosphate and inositol hexakisphosphate. Inositol metabolism plays a role in the development of the neural tube. Disruptions in this gene are thought to be associated with neural tube defects. A pseudogene of this gene has been identified on chromosome X. [provided by RefSeq, Jul 2016]