PPWD1 (NM_015342) Human Tagged ORF Clone

CAT#: RC204570

PPWD1 (Myc-DDK-tagged)-Human peptidylprolyl isomerase domain and WD repeat containing 1 (PPWD1)

Product Images

Western blot validation of overexpression lysate (Cat# LY414604) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204570 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PPWD1 protein (Cat# TP304570). The protein was produced from HEK293T cells transfected with PPWD1 cDNA clone (Cat# RC204570) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PPWD1
• Synonyms: KIAA0073
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204570 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGAAAGTGGTAGCGATTTTCAGCAGAGACGTAGAAGGCGCCGGGACCCGGAGGAACCGGAAA
  AAACAGAACTCAGCGAAAGAGAGCTGGCAGTAGCAGTGGCGGTGTCCCAGGAGAACGATGAGGAGAACGA
  AGAGCGCTGGGTTGGACCTTTACCTGTGGAGGCAACACTGGCCAAGAAGAGGAAAGTCTTAGAGTTTGAA
  AGAGTCTATCTTGATAATCTCCCCAGTGCATCCATGTATGAGCGCAGTTACATGCATAGAGATGTTATCA
  CCCATGTGGTATGCACCAAAACAGATTTTATTATTACTGCCAGTCATGATGGACATGTCAAGTTCTGGAA
  AAAAATAGAAGAGGGAATTGAATTTGTTAAACATTTTCGTAGTCACCTGGGAGTTATTGAGAGTATTGCA
  GTTAGCTCTGAGGGAGCATTGTTCTGTTCTGTGGGTGATGATAAAGCAATGAAGGTGTTTGATGTAGTGA
  ACTTTGACATGATCAACATGCTGAAACTTGGCTATTTTCCTGGACAGTGTGAGTGGATCTATTGCCCAGG
  GGATGCAATTTCTTCAGTTGCTGCTTCCGAAAAGAGTACAGGAAAAATTTTCATTTATGATGGCCGAGGA
  GATAACCAGCCACTTCATATTTTTGACAAACTCCATACATCACCTCTTACTCAGATACGGCTAAACCCAG
  TTTACAAAGCAGTAGTGTCTTCTGACAAATCTGGGATGATTGAATACTGGACTGGGCCTCCTCATGAATA
  TAAATTCCCCAAAAATGTGAACTGGGAATATAAAACTGACACTGATTTATATGAATTTGCCAAGTGTAAG
  GCTTATCCAACCAGCGTATGTTTTTCACCAGATGGGAAGAAAATAGCTACTATTGGTTCTGATAGAAAAG
  TTAGAATTTTCAGATTTGTAACTGGAAAACTCATGAGAGTCTTTGATGAATCACTAAGCATGTTTACTGA
  ACTGCAACAGATGAGGCAACAGTTACCAGACATGGAATTTGGCCGACGAATGGCTGTAGAACGTGAGTTG
  GAGAAGGTTGATGCTGTAAGATTAATTAATATAGTTTTTGATGAAACTGGACACTTCGTGCTGTATGGAA
  CAATGCTGGGCATTAAAGTTATAAATGTAGAGACAAACCGGTGTGTGCGGATTTTAGGCAAACAAGAAAA
  TATTAGAGTGATGCAATTGGCTTTGTTCCAGGGGATAGCCAAAAAGCATCGTGCTGCAACTACTATAGAA
  ATGAAAGCTTCTGAAAATCCTGTTCTTCAGAATATTCAAGCTGACCCAACAATAGTCTGTACATCATTCA
  AAAAGAATAGATTTTATATGTTTACCAAACGAGAACCAGAAGATACGAAAAGTGCAGATTCTGATCGAGA
  TGTTTTTAATGAGAAACCTTCTAAAGAAGAAGTCATGGCAGCTACTCAAGCTGAAGGACCTAAACGAGTT
  TCGGACAGTGCCATTATCCACACCAGCATGGGAGACATTCACACCAAACTTTTTCCTGTTGAGTGCCCTA
  AGACAGTGGAAAACTTCTGTGTTCACAGCAGAAATGGTTATTATAATGGGCATACATTTCACCGTATAAT
  TAAGGGCTTTATGATTCAGACTGGAGATCCAACAGGTACTGGTATGGGAGGAGAAAGCATATGGGGAGGA
  GAATTTGAAGATGAATTTCATTCAACATTACGACATGACAGGCCGTACACACTCAGCATGGCTAACGCGG
  GATCAAATACTAATGGATCCCAGTTTTTCATAACGGTAGTACCAACGCCTTGGCTTGATAATAAGCATAC
  AGTATTTGGACGAGTGACTAAAGGAATGGAAGTTGTACAGAGGATCTCCAACGTCAAAGTCAATCCCAAA
  ACAGATAAGCCCTATGAGGATGTCAGCATCATAAATATTACTGTCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204570 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAESGSDFQQRRRRRRDPEEPEKTELSERELAVAVAVSQENDEENEERWVGPLPVEATLAKKRKVLEFE
  RVYLDNLPSASMYERSYMHRDVITHVVCTKTDFIITASHDGHVKFWKKIEEGIEFVKHFRSHLGVIESIA
  VSSEGALFCSVGDDKAMKVFDVVNFDMINMLKLGYFPGQCEWIYCPGDAISSVAASEKSTGKIFIYDGRG
  DNQPLHIFDKLHTSPLTQIRLNPVYKAVVSSDKSGMIEYWTGPPHEYKFPKNVNWEYKTDTDLYEFAKCK
  AYPTSVCFSPDGKKIATIGSDRKVRIFRFVTGKLMRVFDESLSMFTELQQMRQQLPDMEFGRRMAVEREL
  EKVDAVRLINIVFDETGHFVLYGTMLGIKVINVETNRCVRILGKQENIRVMQLALFQGIAKKHRAATTIE
  MKASENPVLQNIQADPTIVCTSFKKNRFYMFTKREPEDTKSADSDRDVFNEKPSKEEVMAATQAEGPKRV
  SDSAIIHTSMGDIHTKLFPVECPKTVENFCVHSRNGYYNGHTFHRIIKGFMIQTGDPTGTGMGGESIWGG
  EFEDEFHSTLRHDRPYTLSMANAGSNTNGSQFFITVVPTPWLDNKHTVFGRVTKGMEVVQRISNVKVNPK
  TDKPYEDVSIINITVK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015342
• ORF Size: 1938 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015342.1, NM_015342.2, NM_015342.3, NP_056157.1
• RefSeq Size: 2186 bp
• RefSeq ORF: 1941 bp
• Locus ID: 23398
• MW: 73.6 kDa
• Gene Summary: Putative peptidylprolyl isomerase (PPIase). PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing. [UniProtKB/Swiss-Prot Function]