FARSLB (FARSB) (NM_005687) Human Tagged ORF Clone

CAT#: RC204639

FARSB (Myc-DDK-tagged)-Human phenylalanyl-tRNA synthetase, beta subunit (FARSB)

Product Images

Western blot validation of overexpression lysate (Cat# LY401736) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204639 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FARSB protein (Cat# TP304639). The protein was produced from HEK293T cells transfected with FARSB cDNA clone (Cat# RC204639) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FARSB
• Synonyms: FARSLB; FRSB; HSPC173; NEDBLLA; PheHB; PheRS; RILDBC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204639 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGACTGTCAGCGTGAAGCGTGATCTGCTCTTCCAAGCCCTGGGCCGCACCTACACTGACGAAGAAT
  TTGATGAACTATGTTTTGAATTTGGTCTGGAGCTTGATGAAATTACATCTGAGAAGGAAATAATAAGTAA
  AGAACAAGGTAATGTAAAGGCAGCAGGAGCCTCTGATGTTGTTCTTTACAAAATTGACGTCCCTGCCAAT
  AGATATGATCTCCTGTGTCTGGAAGGATTGGTTCGAGGACTTCAGGTCTTCAAAGAAAGGATAAAGGCTC
  CAGTGTATAAACGGGTAATGCCTGATGGAAAAATCCAGAAATTGATTATCACAGAAGAGACAGCTAAGAT
  ACGTCCTTTTGCGGTAGCAGCAGTTCTCCGTAATATAAAGTTTACTAAAGATCGATATGACAGCTTCATT
  GAACTTCAGGAGAAATTACATCAGAATATTTGCAGGAAAAGAGCACTGGTTGCCATTGGTACCCATGATT
  TGGACACTTTGTCGGGCCCATTTACTTATACTGCAAAGCGTCCTTCAGATATCAAATTCAAGCCTCTAAA
  TAAGACCAAGGAGTATACAGCCTGTGAACTGATGAACATATACAAGACTGACAATCACCTGAAACATTAT
  TTACATATCATTGAAAACAAACCCCTGTATCCAGTTATCTATGATAGCAATGGTGTCGTCCTTTCAATGC
  CTCCCATCATCAATGGGGATCATTCCAGAATAACAGTAAATACTAGAAATATTTTTATTGAATGCACGGG
  AACTGACTTTACTAAGGCAAAAATAGTTCTTGATATTATTGTCACCATGTTCAGTGAATATTGTGAGAAT
  CAATTTACGGTCGAAGCTGCTGAAGTGGTTTTTCCTAATGGAAAATCACATACCTTTCCAGAATTAGCTT
  ACCGAAAGGAGATGGTGAGAGCTGACCTAATTAACAAAAAAGTTGGAATCAGAGAAACTCCAGAAAATCT
  TGCCAAACTTCTGACCAGGATGTATTTAAAATCAGAAGTCATAGGTGATGGGAATCAGATTGAGATTGAA
  ATCCCTCCAACCAGAGCTGACATTATCCATGCATGTGATATTGTAGAAGATGCAGCTATTGCTTATGGAT
  ATAACAACATTCAGATGACTCTCCCGAAAACTTACACCATAGCTAATCAATTTCCTCTTAATAAGCTCAC
  TGAACTTCTCCGACATGACATGGCAGCCGCTGGCTTCACTGAAGCACTTACCTTTGCCCTGTGCTCCCAA
  GAAGATATTGCTGATAAACTAGGTGTGGATATCTCTGCAACAAAGGCAGTCCACATAAGTAATCCTAAAA
  CAGCTGAATTTCAGGTGGCACGCACTACCCTTCTTCCTGGCCTCCTGAAGACCATAGCAGCAAATCGTAA
  GATGCCCCTTCCACTGAAACTGTTTGAAATCTCTGACATTGTAATAAAAGATTCTAATACAGATGTAGGT
  GCAAAAAACTACAGACATCTCTGTGCTGTTTATTACAACAAGAATCCTGGGTTTGAGATCATTCATGGGC
  TGCTGGACAGAATTATGCAGTTGCTCGATGTGCCTCCTGGTGAAGACAAGGGGGGATATGTGATCAAAGC
  ATCAGAAGGGCCTGCTTTCTTCCCCGGGCGATGTGCAGAGATTTTTGCCAGGGGTCAAAGCGTCGGGAAG
  CTTGGGGTCCTTCATCCTGACGTTATCACCAAATTTGAGCTGACCATGCCCTGCTCCTCCCTAGAAATCA
  ATATTGGACCCTTTTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204639 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPTVSVKRDLLFQALGRTYTDEEFDELCFEFGLELDEITSEKEIISKEQGNVKAAGASDVVLYKIDVPAN
  RYDLLCLEGLVRGLQVFKERIKAPVYKRVMPDGKIQKLIITEETAKIRPFAVAAVLRNIKFTKDRYDSFI
  ELQEKLHQNICRKRALVAIGTHDLDTLSGPFTYTAKRPSDIKFKPLNKTKEYTACELMNIYKTDNHLKHY
  LHIIENKPLYPVIYDSNGVVLSMPPIINGDHSRITVNTRNIFIECTGTDFTKAKIVLDIIVTMFSEYCEN
  QFTVEAAEVVFPNGKSHTFPELAYRKEMVRADLINKKVGIRETPENLAKLLTRMYLKSEVIGDGNQIEIE
  IPPTRADIIHACDIVEDAAIAYGYNNIQMTLPKTYTIANQFPLNKLTELLRHDMAAAGFTEALTFALCSQ
  EDIADKLGVDISATKAVHISNPKTAEFQVARTTLLPGLLKTIAANRKMPLPLKLFEISDIVIKDSNTDVG
  AKNYRHLCAVYYNKNPGFEIIHGLLDRIMQLLDVPPGEDKGGYVIKASEGPAFFPGRCAEIFARGQSVGK
  LGVLHPDVITKFELTMPCSSLEINIGPFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005687
• ORF Size: 1767 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005687.1, NM_005687.2, NM_005687.3, NM_005687.4, NP_005678.2, NP_005678.3
• RefSeq Size: 2233 bp
• RefSeq ORF: 1770 bp
• Locus ID: 10056
• Domains: B3_4, B5
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• MW: 66.1 kDa
• Gene Summary: This gene encodes a highly conserved enzyme that belongs to the aminoacyl-tRNA synthetase class IIc subfamily. This enzyme comprises the regulatory beta subunits that form a tetramer with two catalytic alpha subunits. In the presence of ATP, this tetramer is responsible for attaching L-phenylalanine to the terminal adenosine of the appropriate tRNA. A pseudogene located on chromosome 10 has been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]