Langerin (CD207) (NM_015717) Human Tagged ORF Clone

CAT#: RC204669

CD207 (Myc-DDK-tagged)-Human CD207 molecule, langerin (CD207)

Product Images

Western blot validation of overexpression lysate (Cat# LY402461) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204669 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CD207 protein (Cat# TP304669). The protein was produced from HEK293T cells transfected with CD207 cDNA clone (Cat# RC204669) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CD207
• Synonyms: CLEC4K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204669 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGTGGAGAAGGAGGCCCCTGATGCGCACTTCACTGTGGACAAACAGAACATCTCCCTCTGGCCCC
  GAGAGCCTCCTCCCAAGTCCGGTCCATCTCTGGTCCCGGGGAAAACACCCACAGTCCGTGCTGCATTAAT
  CTGCCTGACGCTGGTCCTGGTCGCCTCCGTCCTGCTGCAGGCCGTCCTTTATCCCCGGTTTATGGGCACC
  ATATCAGATGTAAAGACCAATGTCCAGTTGCTGAAAGGTCGTGTGGACAACATCAGCACCCTGGATTCTG
  AAATTAAAAAGAATAGTGACGGCATGGAGGCAGCTGGCGTTCAGATCCAGATGGTGAATGAGAGCCTGGG
  TTATGTGCGTTCTCAGTTCCTGAAGTTAAAAACCAGTGTGGAGAAGGCCAACGCACAGATCCAGATCTTA
  ACAAGAAGTTGGGAAGAAGTCAGTACCTTAAATGCCCAAATCCCAGAGTTAAAAAGTGATTTGGAGAAAG
  CCAGTGCTTTAAATACAAAGATCCGGGCACTCCAGGGCAGCTTGGAGAATATGAGCAAGTTGCTCAAACG
  ACAAAATGATATTCTACAGGTGGTTTCTCAAGGCTGGAAGTACTTCAAGGGGAACTTCTATTACTTTTCT
  CTCATTCCAAAGACCTGGTATAGTGCCGAGCAGTTCTGTGTGTCCAGGAATTCACACCTGACCTCGGTGA
  CCTCAGAGAGTGAGCAGGAGTTTCTGTATAAAACAGCGGGGGGACTCATCTACTGGATTGGCCTGACTAA
  AGCAGGGATGGAAGGGGACTGGTCCTGGGTGGATGACACGCCATTCAACAAGGTCCAAAGTGCGAGGTTC
  TGGATTCCAGGTGAGCCCAACAATGCTGGGAACAATGAACACTGTGGCAATATAAAGGCTCCCTCACTTC
  AGGCCTGGAATGATGCCCCATGTGACAAAACGTTTCTTTTCATTTGTAAGCGACCCTATGTCCCATCAGA
  ACCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204669 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTVEKEAPDAHFTVDKQNISLWPREPPPKSGPSLVPGKTPTVRAALICLTLVLVASVLLQAVLYPRFMGT
  ISDVKTNVQLLKGRVDNISTLDSEIKKNSDGMEAAGVQIQMVNESLGYVRSQFLKLKTSVEKANAQIQIL
  TRSWEEVSTLNAQIPELKSDLEKASALNTKIRALQGSLENMSKLLKRQNDILQVVSQGWKYFKGNFYYFS
  LIPKTWYSAEQFCVSRNSHLTSVTSESEQEFLYKTAGGLIYWIGLTKAGMEGDWSWVDDTPFNKVQSARF
  WIPGEPNNAGNNEHCGNIKAPSLQAWNDAPCDKTFLFICKRPYVPSEP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015717
• ORF Size: 984 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015717.1, NM_015717.2, NM_015717.3, NM_015717.4, NP_056532.1
• RefSeq Size: 1886 bp
• RefSeq ORF: 987 bp
• Locus ID: 50489
• Protein Families: Druggable Genome, Transmembrane
• MW: 36.7 kDa
• Gene Summary: The protein encoded by this gene is expressed only in Langerhans cells which are immature dendritic cells of the epidermis and mucosa. It is localized in the Birbeck granules, organelles present in the cytoplasm of Langerhans cells and consisting of superimposed and zippered membranes. It is a C-type lectin with mannose binding specificity, and it has been proposed that mannose binding by this protein leads to internalization of antigen into Birbeck granules and providing access to a nonclassical antigen-processing pathway. Mutations in this gene result in Birbeck granules deficiency or loss of sugar binding activity. [provided by RefSeq, Aug 2010]