PGCP (CPQ) (NM_016134) Human Tagged ORF Clone

CAT#: RC204684

CPQ (Myc-DDK-tagged)-Human plasma glutamate carboxypeptidase (PGCP)

Product Images

Western blot validation of overexpression lysate (Cat# LY402505) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204684 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CPQ protein (Cat# TP304684). The protein was produced from HEK293T cells transfected with CPQ cDNA clone (Cat# RC204684) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CPQ
• Synonyms: LDP; PGCP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204684 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAATTCCTTATCTTCGCATTTTTCGGTGGTGTTCACCTTTTATCCCTGTGCTCTGGGAAAGCTATAT
  GCAAGAATGGCATCTCTAAGAGGACTTTTGAAGAAATAAAAGAAGAAATAGCCAGCTGTGGAGATGTTGC
  TAAAGCAATCATCAACCTAGCTGTTTATGGTAAAGCCCAGAACAGATCCTATGAGCGATTGGCACTTCTG
  GTTGATACTGTTGGACCCAGACTGAGTGGCTCCAAGAACCTAGAAAAAGCCATCCAAATTATGTACCAAA
  ACCTGCAGCAAGATGGGCTGGAGAAAGTTCACCTGGAGCCAGTGAGAATACCCCACTGGGAGAGGGGAGA
  AGAATCAGCTGTGATGCTGGAGCCAAGAATTCATAAGATAGCCATCCTGGGTCTTGGCAGCAGCATTGGG
  ACTCCTCCAGAAGGCATTACAGCAGAAGTTCTGGTGGTGACCTCTTTCGATGAACTGCAGAGAAGGGCCT
  CAGAAGCAAGAGGGAAGATTGTTGTTTATAACCAACCTTACATCAACTACTCAAGGACGGTGCAATACCG
  AACGCAGGGGGCGGTGGAAGCTGCCAAGGTTGGGGCTTTGGCATCTCTCATTCGATCCGTGGCCTCCTTC
  TCCATCTACAGTCCTCACACAGGTATTCAGGAATACCAGGATGGCGTGCCCAAGATTCCAACAGCCTGTA
  TTACGGTGGAAGATGCAGAAATGATGTCAAGAATGGCTTCTCATGGGATCAAAATTGTCATTCAGCTAAA
  GATGGGGGCAAAGACCTACCCAGATACTGATTCCTTCAACACTGTAGCAGAGATCACTGGGAGCAAATAT
  CCAGAACAGGTTGTACTGGTCAGTGGACATCTGGACAGCTGGGATGTTGGGCAGGGTGCCATGGATGATG
  GCGGTGGAGCCTTTATATCATGGGAAGCACTCTCACTTATTAAAGATCTTGGGCTGCGTCCAAAGAGGAC
  TCTGCGGCTGGTGCTCTGGACTGCAGAAGAACAAGGTGGAGTTGGTGCCTTCCAGTATTATCAGTTACAC
  AAGGTAAATATTTCCAACTACAGTCTGGTGATGGAGTCTGACGCAGGAACCTTCTTACCCACTGGGCTGC
  AATTCACTGGCAGTGAAAAGGCCAGGGCCATCATGGAGGAGGTTATGAGCCTGCTGCAGCCCCTCAATAT
  CACTCAGGTCCTGAGCCATGGAGAAGGGACAGACATCAACTTTTGGATCCAAGCTGGAGTGCCTGGAGCC
  AGTCTACTTGATGACTTATACAAGTATTTCTTCTTCCATCACTCCCACGGAGACACCATGACTGTCATGG
  ATCCAAAGCAGATGAATGTTGCTGCTGCTGTTTGGGCTGTTGTTTCTTATGTTGTTGCAGACATGGAAGA
  AATGCTGCCTAGGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204684 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKFLIFAFFGGVHLLSLCSGKAICKNGISKRTFEEIKEEIASCGDVAKAIINLAVYGKAQNRSYERLALL
  VDTVGPRLSGSKNLEKAIQIMYQNLQQDGLEKVHLEPVRIPHWERGEESAVMLEPRIHKIAILGLGSSIG
  TPPEGITAEVLVVTSFDELQRRASEARGKIVVYNQPYINYSRTVQYRTQGAVEAAKVGALASLIRSVASF
  SIYSPHTGIQEYQDGVPKIPTACITVEDAEMMSRMASHGIKIVIQLKMGAKTYPDTDSFNTVAEITGSKY
  PEQVVLVSGHLDSWDVGQGAMDDGGGAFISWEALSLIKDLGLRPKRTLRLVLWTAEEQGGVGAFQYYQLH
  KVNISNYSLVMESDAGTFLPTGLQFTGSEKARAIMEEVMSLLQPLNITQVLSHGEGTDINFWIQAGVPGA
  SLLDDLYKYFFFHHSHGDTMTVMDPKQMNVAAAVWAVVSYVVADMEEMLPRS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016134
• ORF Size: 1416 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016134.1, NM_016134.2, NM_016134.3, NP_057218.1
• RefSeq Size: 1949 bp
• RefSeq ORF: 1419 bp
• Locus ID: 10404
• Domains: Peptidase_M28
• Protein Families: Protease
• MW: 51.9 kDa
• Gene Summary: This gene encodes a metallopeptidase that belongs to the peptidase M28 family. The encoded protein may catalyze the cleavage of dipeptides with unsubstituted terminals into amino acids. [provided by RefSeq, Jul 2013]