Complement C9 (C9) (NM_001737) Human Tagged ORF Clone

CAT#: RC204712

C9 (Myc-DDK-tagged)-Human complement component 9 (C9)

Product Images

Western blot validation of overexpression lysate (Cat# LY419774) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204712 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C9
• Synonyms: ARMD15; C9D
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204712 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGCCTGCCGGAGCTTTGCAGTTGCAATCTGCATTTTAGAAATAAGCATCCTCACAGCACAGTACA
  CGACCAGTTATGACCCAGAGCTAACAGAAAGCAGTGGCTCTGCATCACACATAGACTGCAGAATGAGCCC
  CTGGAGTGAATGGTCACAATGCGATCCTTGTCTCAGACAAATGTTTCGTTCAAGAAGCATTGAGGTCTTT
  GGACAATTTAATGGGAAAAGATGCACCGACGCTGTGGGAGACAGACGACAGTGTGTGCCCACAGAGCCCT
  GTGAGGATGCTGAGGATGACTGCGGAAATGACTTTCAATGCAGTACAGGCAGATGCATAAAGATGCGACT
  TCGGTGTAATGGTGACAATGACTGCGGAGACTTTTCAGATGAGGATGATTGTGAAAGTGAGCCCCGTCCC
  CCCTGCAGAGACAGAGTGGTAGAAGAGTCTGAGCTGGCACGAACAGCAGGCTATGGGATCAACATTTTAG
  GGATGGATCCCCTAAGCACACCTTTTGACAATGAGTTCTACAATGGACTCTGTAACCGGGATCGGGATGG
  AAACACTCTGACATACTACCGAAGACCTTGGAACGTGGCTTCTTTGATCTATGAAACCAAAGGCGAGAAA
  AATTTCAGAACCGAACATTACGAAGAACAAATTGAAGCATTTAAAAGTATCATCCAAGAGAAGACATCAA
  ATTTTAATGCAGCTATATCTCTAAAATTTACACCCACTGAAACAAATAAAGCTGAACAATGTTGTGAGGA
  AACAGCCTCCTCAATTTCTTTACATGGCAAGGGTAGTTTTCGGTTTTCATATTCCAAAAATGAAACTTAC
  CAACTATTTTTGTCATATTCTTCAAAGAAGGAAAAAATGTTTCTGCATGTGAAAGGAGAAATTCATCTGG
  GAAGATTTGTAATGAGAAATCGCGATGTTGTGCTCACAACAACTTTTGTGGATGATATAAAAGCTTTGCC
  AACTACCTATGAAAAGGGAGAATATTTTGCCTTTTTGGAAACCTATGGAACTCACTACAGTAGCTCTGGG
  TCTCTAGGAGGACTCTATGAACTAATATATGTTTTGGATAAAGCTTCCATGAAGCGGAAAGGTGTTGAAC
  TAAAAGACATAAAGAGATGCCTTGGGTATCATCTGGATGTATCTCTGGCTTTCTCTGAAATCTCTGTTGG
  AGCTGAATTTAATAAAGATGATTGTGTAAAGAGGGGAGAGGGTAGAGCTGTAAACATCACCAGTGAAAAC
  CTCATAGATGATGTTGTTTCACTCATAAGAGGTGGAACCAGAAAATATGCATTTGAACTGAAAGAAAAGC
  TTCTCCGAGGAACCGTGATTGATGTGACTGACTTTGTCAACTGGGCCTCTTCCATAAATGATGCTCCTGT
  TCTCATTAGTCAAAAACTGTCTCCTATATATAATCTGGTTCCAGTGAAAATGAAAAATGCACACCTAAAG
  AAACAAAACTTGGAAAGAGCCATTGAAGACTATATCAATGAATTTAGTGTAAGAAAATGCCACACATGCC
  AAAATGGAGGTACAGTGATTCTAATGGATGGAAAGTGTTTGTGTGCCTGCCCATTCAAATTTGAGGGAAT
  TGCCTGTGAAATCAGTAAACAAAAAATTTCTGAAGGATTGCCAGCCCTAGAGTTCCCCAATGAAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204712 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSACRSFAVAICILEISILTAQYTTSYDPELTESSGSASHIDCRMSPWSEWSQCDPCLRQMFRSRSIEVF
  GQFNGKRCTDAVGDRRQCVPTEPCEDAEDDCGNDFQCSTGRCIKMRLRCNGDNDCGDFSDEDDCESEPRP
  PCRDRVVEESELARTAGYGINILGMDPLSTPFDNEFYNGLCNRDRDGNTLTYYRRPWNVASLIYETKGEK
  NFRTEHYEEQIEAFKSIIQEKTSNFNAAISLKFTPTETNKAEQCCEETASSISLHGKGSFRFSYSKNETY
  QLFLSYSSKKEKMFLHVKGEIHLGRFVMRNRDVVLTTTFVDDIKALPTTYEKGEYFAFLETYGTHYSSSG
  SLGGLYELIYVLDKASMKRKGVELKDIKRCLGYHLDVSLAFSEISVGAEFNKDDCVKRGEGRAVNITSEN
  LIDDVVSLIRGGTRKYAFELKEKLLRGTVIDVTDFVNWASSINDAPVLISQKLSPIYNLVPVKMKNAHLK
  KQNLERAIEDYINEFSVRKCHTCQNGGTVILMDGKCLCACPFKFEGIACEISKQKISEGLPALEFPNEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001737
• ORF Size: 1677 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001737.1, NM_001737.2, NM_001737.3, NM_001737.4, NP_001728.1
• RefSeq Size: 2693 bp
• RefSeq ORF: 1680 bp
• Locus ID: 735
• Cytogenetics: 5p13.1
• Domains: tsp_1, MACPF, ldl_recept_a
• Protein Families: Druggable Genome
• Protein Pathways: Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus
• MW: 63.2 kDa
• Gene Summary: 'This gene encodes the final component of the complement system. It participates in the formation of the Membrane Attack Complex (MAC). The MAC assembles on bacterial membranes to form a pore, permitting disruption of bacterial membrane organization. Mutations in this gene cause component C9 deficiency. [provided by RefSeq, Feb 2009]'