C3AR1 (NM_004054) Human Tagged ORF Clone

CAT#: RC204730

C3AR1 (Myc-DDK-tagged)-Human complement component 3a receptor 1 (C3AR1)

Product Images

Western blot validation of overexpression lysate (Cat# LY401314) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204730 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified C3AR1 protein (Cat# TP304730). The protein was produced from HEK293T cells transfected with C3AR1 cDNA clone (Cat# RC204730) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C3AR1
• Synonyms: AZ3B; C3AR; HNFAG09
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204730 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCTTTCTCTGCTGAGACCAATTCAACTGACCTACTCTCACAGCCATGGAATGAGCCCCCAGTAA
  TTCTCTCCATGGTCATTCTCAGCCTTACTTTTTTACTGGGATTGCCAGGCAATGGGCTGGTGCTGTGGGT
  GGCTGGCCTGAAGATGCAGCGGACAGTGAACACAATTTGGTTCCTCCACCTCACCTTGGCGGACCTCCTC
  TGCTGCCTCTCCTTGCCCTTCTCGCTGGCTCACTTGGCTCTCCAGGGACAGTGGCCCTACGGCAGGTTCC
  TATGCAAGCTCATCCCCTCCATCATTGTCCTCAACATGTTTGCCAGTGTCTTCCTGCTTACTGCCATTAG
  CCTGGATCGCTGTCTTGTGGTATTCAAGCCAATCTGGTGTCAGAATCATCGCAATGTAGGGATGGCCTGC
  TCTATCTGTGGATGTATCTGGGTGGTGGCTTTTGTGATGTGCATTCCTGTGTTCGTGTACCGGGAAATCT
  TCACTACAGACAACCATAATAGATGTGGCTACAAATTTGGTCTCTCCAGCTCATTAGATTATCCAGACTT
  TTATGGAGATCCACTAGAAAACAGGTCTCTTGAAAACATTGTTCAGCCGCCTGGAGAAATGAATGATAGG
  TTAGATCCTTCCTCTTTCCAAACAAATGATCATCCTTGGACAGTCCCCACTGTCTTCCAACCTCAAACAT
  TTCAAAGACCTTCTGCAGATTCACTCCCTAGGGGTTCTGCTAGGTTAACAAGTCAAAATCTGTATTCTAA
  TGTATTTAAACCTGCTGATGTGGTCTCACCTAAAATCCCCAGTGGGTTTCCTATTGAAGATCACGAAACC
  AGCCCACTGGATAACTCTGATGCTTTTCTCTCTACTCATTTAAAGCTGTTCCCTAGCGCTTCTAGCAATT
  CCTTCTACGAGTCTGAGCTACCACAAGGTTTCCAGGATTATTACAATTTAGGCCAATTCACAGATGACGA
  TCAAGTGCCAACACCCCTCGTGGCAATAACGATCACTAGGCTAGTGGTGGGTTTCCTGCTGCCCTCTGTT
  ATCATGATAGCCTGTTACAGCTTCATTGTCTTCCGAATGCAAAGGGGCCGCTTCGCCAAGTCTCAGAGCA
  AAACCTTTCGAGTGGCCGTGGTGGTGGTGGCTGTCTTTCTTGTCTGCTGGACTCCATACCACATTTTTGG
  AGTCCTGTCATTGCTTACTGACCCAGAAACTCCCTTGGGGAAAACTCTGATGTCCTGGGATCATGTATGC
  ATTGCTCTAGCATCTGCCAATAGTTGCTTTAATCCCTTCCTTTATGCCCTCTTGGGGAAAGATTTTAGGA
  AGAAAGCAAGGCAGTCCATTCAGGGAATTCTGGAGGCAGCCTTCAGTGAGGAGCTCACACGTTCCACCCA
  CTGTCCCTCAAACAATGTCATTTCAGAAAGAAATAGTACAACTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204730 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASFSAETNSTDLLSQPWNEPPVILSMVILSLTFLLGLPGNGLVLWVAGLKMQRTVNTIWFLHLTLADLL
  CCLSLPFSLAHLALQGQWPYGRFLCKLIPSIIVLNMFASVFLLTAISLDRCLVVFKPIWCQNHRNVGMAC
  SICGCIWVVAFVMCIPVFVYREIFTTDNHNRCGYKFGLSSSLDYPDFYGDPLENRSLENIVQPPGEMNDR
  LDPSSFQTNDHPWTVPTVFQPQTFQRPSADSLPRGSARLTSQNLYSNVFKPADVVSPKIPSGFPIEDHET
  SPLDNSDAFLSTHLKLFPSASSNSFYESELPQGFQDYYNLGQFTDDDQVPTPLVAITITRLVVGFLLPSV
  IMIACYSFIVFRMQRGRFAKSQSKTFRVAVVVVAVFLVCWTPYHIFGVLSLLTDPETPLGKTLMSWDHVC
  IALASANSCFNPFLYALLGKDFRKKARQSIQGILEAAFSEELTRSTHCPSNNVISERNSTTV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004054
• ORF Size: 1446 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004054.1, NM_004054.2, NM_004054.3, NP_004045.1
• RefSeq Size: 1985 bp
• RefSeq ORF: 1449 bp
• Locus ID: 719
• Cytogenetics: 12p13.31
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Complement and coagulation cascades, Neuroactive ligand-receptor interaction
• MW: 53.9 kDa
• Gene Summary: 'C3a is an anaphylatoxin released during activation of the complement system. The protein encoded by this gene is an orphan G protein-coupled receptor for C3a. Binding of C3a by the encoded receptor activates chemotaxis, granule enzyme release, superoxide anion production, and bacterial opsonization. [provided by RefSeq, May 2016]'