RPL10L (NM_080746) Human Tagged ORF Clone

CAT#: RC204751

RPL10L (Myc-DDK-tagged)-Human ribosomal protein L10-like (RPL10L)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RPL10L
• Synonyms: RPL10_5_1358
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204751 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCGCCGTCCAGCTCGCTGTTACCGGTATTGTAAGAACAAGCCGTACCCAAAATCTCGTTTCTGCC
  GAGGGGTTCCTGATGCCAAGATCCGCATCTTTGACCTGGGTAGAAAGAAGGCAAAAGTGGATGAGTTCCC
  ACTCGGTGGCCACATGGTGTCTGATGAATATGAGCAGCTGTCTTCTGAAGCCCTGGAGGCCGCCCGTATT
  TGTGCCAACAAATACATGGTGAAAAGTTGTGGCAGAGATGGCTTTCACATGCGAGTGCGGCTCCATCCCT
  TCCATGTCATCCGCATCAACAAGATGTTGTCCTGTGCTGGGGCTGACAGGCTCCAGACAGGTATGCGAGG
  TGCCTTTGGAAAACCCCAGGGTACTGTAGCCCGGGTCCACATTGGTCAAGTCATCATGTCCATCCGCACC
  AAGCTTCAGAACGAGGAGCATGTGATTGAAGCCTTGCGCAGGGCCAAGTTCAAGTTCCCTGGACGCCAGA
  AGATTCATATCTCCAAGAAGTGGGGCTTCACGAAGTTTAATGCTGACGAATTTGAAGACATGGTGGCCAA
  GAAGTGCCTCATCCCTGATGGTTGTGGAGTCAAGTACGTTCCCAGTCATGGCCCCTTGGACAAGTGGCGG
  GTTCTGCACTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204751 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGRRPARCYRYCKNKPYPKSRFCRGVPDAKIRIFDLGRKKAKVDEFPLGGHMVSDEYEQLSSEALEAARI
  CANKYMVKSCGRDGFHMRVRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVHIGQVIMSIRT
  KLQNEEHVIEALRRAKFKFPGRQKIHISKKWGFTKFNADEFEDMVAKKCLIPDGCGVKYVPSHGPLDKWR
  VLHS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080746
• ORF Size: 642 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080746.1, NM_080746.2, NP_542784.1
• RefSeq Size: 838 bp
• RefSeq ORF: 645 bp
• Locus ID: 140801
• Domains: Ribosomal_L10e
• Protein Pathways: Ribosome
• MW: 24.5 kDa
• Gene Summary: This gene encodes a protein sharing sequence similarity with ribosomal protein L10. It is not currently known whether the encoded protein is a functional ribosomal protein or whether it has evolved a function that is independent of the ribosome. This gene is intronless. [provided by RefSeq, Jul 2008]