GNPDA1 (NM_005471) Human Tagged ORF Clone

CAT#: RC204763

GNPDA1 (Myc-DDK-tagged)-Human glucosamine-6-phosphate deaminase 1 (GNPDA1)

Product Images

Western blot validation of overexpression lysate (Cat# LY417282) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204763 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GNPDA1
• Synonyms: GNP1; GNPDA; GNPI; GPI; HLN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204763 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTCATCATCCTGGAGCACTATTCTCAGGCGAGCGAGTGGGCGGCTAAATACATCAGGAACCGCA
  TCATCCAGTTTAACCCAGGGCCAGAGAAGTACTTCACCCTGGGGCTCCCCACTGGGAGTACCCCACTTGG
  CTGCTACAAGAAGCTGATTGAATACTATAAGAATGGGGACCTGTCCTTTAAATATGTGAAGACCTTCAAC
  ATGGATGAGTACGTGGGCCTTCCTCGAGACCACCCGGAGAGTTACCACTCCTTCATGTGGAACAACTTCT
  TCAAGCACATTGACATCCACCCAGAAAACACCCACATTCTGGATGGGAATGCAGTCGACCTACAGGCAGA
  ATGTGATGCCTTTGAAGAGAAGATCAAGGCTGCAGGTGGGATCGAGCTATTTGTTGGAGGCATCGGCCCT
  GATGGACACATTGCCTTCAACGAGCCAGGCTCCAGTCTGGTGTCCAGGACCCGTGTGAAGACGCTGGCCA
  TGGATACCATCCTGGCCAATGCTAGGTTCTTCGATGGAGAACTCACCAAGGTGCCCACCATGGCCTTGAC
  GGTGGGGGTGGGCACTGTCATGGATGCTAGAGAGGTGATGATCCTTATCACAGGTGCTCACAAGGCATTT
  GCTCTGTACAAGGCCATCGAGGAGGGAGTGAACCACATGTGGACCGTGTCTGCCTTCCAGCAGCATCCCC
  GCACCGTGTTTGTGTGTGACGAGGATGCCACCTTGGAGCTGAAAGTGAAGACTGTCAAGTATTTCAAAGG
  TTTAATGCTTGTTCATAACAAGTTGGTGGACCCCTTGTACAGTATCAAAGAGAAAGAAACTGAGAAAAGC
  CAATCTTCGAAGAAACCATACAGCGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204763 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKLIILEHYSQASEWAAKYIRNRIIQFNPGPEKYFTLGLPTGSTPLGCYKKLIEYYKNGDLSFKYVKTFN
  MDEYVGLPRDHPESYHSFMWNNFFKHIDIHPENTHILDGNAVDLQAECDAFEEKIKAAGGIELFVGGIGP
  DGHIAFNEPGSSLVSRTRVKTLAMDTILANARFFDGELTKVPTMALTVGVGTVMDAREVMILITGAHKAF
  ALYKAIEEGVNHMWTVSAFQQHPRTVFVCDEDATLELKVKTVKYFKGLMLVHNKLVDPLYSIKEKETEKS
  QSSKKPYSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005471
• ORF Size: 867 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005471.1, NM_005471.2, NM_005471.3, NM_005471.4, NP_005462.1
• RefSeq Size: 2311 bp
• RefSeq ORF: 870 bp
• Locus ID: 10007
• Domains: Glucosamine_iso
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Metabolic pathways
• MW: 32.7 kDa
• Gene Summary: Glucosamine-6-phosphate deaminase (EC 3.5.99.6) is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium (Arreola et al., 2003 [PubMed 12965206]). [supplied by OMIM, Jan 2010]