TCIM (NM_020130) Human Tagged ORF Clone
CAT#: RC204798
C8orf4 (Myc-DDK-tagged)-Human chromosome 8 open reading frame 4 (C8orf4)
"NM_020130" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | TCIM |
Synonyms | C8orf4; TC-1; TC1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC204798 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGCAAAGCGAAGCCACCAAGCCATCATCATGTCCACGTCGCTACGAGTCAGCCCATCCATCCATG GCTACCACTTCGACACAGCCTCTCGTAAGAAAGCCGTGGGCAACATCTTTGAAAACACAGACCAAGAATC ACTAGAAAGGCTCTTCAGAAACTCTGGAGACAAGAAAGCAGAGGAGAGAGCCAAGATCATTTTTGCCATA GATCAAGATGTGGAGGAGAAAACGCGTGCCCTGATGGCCTTGAAGAAGAGGACAAAAGACAAGCTTTTCC AGTTTCTGAAACTGCGGAAATATTCCATCAAAGTTCAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC204798 protein sequence
Red=Cloning site Green=Tags(s) MKAKRSHQAIIMSTSLRVSPSIHGYHFDTASRKKAVGNIFENTDQESLERLFRNSGDKKAEERAKIIFAI DQDVEEKTRALMALKKRTKDKLFQFLKLRKYSIKVH SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-RsrII Cloning Scheme for this gene Plasmid Map |
ACCN | NM_020130 |
ORF Size | 318 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020130.1, NM_020130.2, NM_020130.3, NM_020130.4, NP_064515.1 |
RefSeq Size | 1841 bp |
RefSeq ORF | 321 bp |
Locus ID | 56892 |
MW | 12.4 kDa |
Gene Summary | This gene encodes a small, monomeric, predominantly unstructured protein that functions as a positive regulator of the Wnt/beta-catenin signaling pathway. This protein interacts with a repressor of beta-catenin mediated transcription at nuclear speckles. It is thought to competitively block interactions of the repressor with beta-catenin, resulting in up-regulation of beta-catenin target genes. The encoded protein may also play a role in the NF-kappaB and ERK1/2 signaling pathways. Expression of this gene may play a role in the proliferation of several types of cancer including thyroid cancer, breast cancer and hematological malignancies. [provided by RefSeq, Nov 2011] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC113184 | C8orf4 (untagged)-Human chromosome 8 open reading frame 4 (C8orf4) |
USD 310.00 |
|
RG204798 | C8orf4 (GFP-tagged) - Human chromosome 8 open reading frame 4 (C8orf4) |
USD 460.00 |
|
RC204798L3 | Lenti ORF clone of Human chromosome 8 open reading frame 4 (C8orf4), Myc-DDK-tagged |
USD 620.00 |
|
RC204798L4 | Lenti ORF clone of Human chromosome 8 open reading frame 4 (C8orf4), mGFP tagged |
USD 620.00 |
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