PRAF1 (POLR1E) (NM_022490) Human Tagged ORF Clone
CAT#: RC204811
POLR1E (Myc-DDK-tagged)-Human polymerase (RNA) I polypeptide E, 53kDa (POLR1E)
"NM_022490" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | POLR1E |
Synonyms | PAF53; PRAF1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC204811 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGAGGTGTTGCCGAGTGCGAGGTGGCAGTATTGTGGGGCGCCCGACGGGAGCCAGAGAGCTG TACTGGTCCAGTTCTCCAACGGGAAGCTACAGAGTCCAGGCAACATGCGCTTTACCTTGTATGAGAACAA AGATTCCACCAACCCCAGGAAGAGGAATCAACGGATCCTGGCAGCTGAAACAGATAGGCTCTCCTATGTG GGAAACAATTTTGGGACTGGAGCCCTCAAATGCAACACTTTGTGCAGGCACTTTGTGGGAATTTTGAACA AGACCTCTGGCCAAATGGAAGTATATGATGCTGAATTGTTCAACATGCAGCCACTATTTTCAGATGTATC AGTTGAGAGTGAACTGGCTCTAGAGAGTCAGACCAAAACTTACAGAGAAAAGATGGATTCTTGTATTGAA GCCTTTGGTACCACTAAACAGAAGCGAGCTCTGAACACCAGGAGAATGAACAGAGTTGGCAATGAATCTT TGAATCGTGCAGTGGCTAAAGCTGCAGAGACTATCATTGATACGAAGGGTGTGACTGCTCTGGTCAGCGA TGCTATCCACAATGACTTGCAAGATGACTCCCTCTACCTTCCTCCCTGCTATGATGATGCAGCCAAGCCT GAAGACGTGTATAAATTTGAAGATCTTCTTTCCCCTGCGGAGTATGAAGCTCTTCAGAGCCCATCTGAAG CTTTCAGGAACGTCACGTCAGAAGAAATACTGAAGATGATTGAGGAGAACAGCCATTGCACCTTTGTCAT AGAAGCGTTGAAGTCTTTGCCATCAGATGTGGAGAGCCGAGACCGCCAGGCCCGATGCATATGGTTTCTG GATACCCTCATCAAATTTCGAGCTCATAGGGTAGTTAAGCGGAAAAGTGCTCTGGGACCTGGAGTTCCCC ACATCATCAACACCAAACTGCTGAAGCACTTTACCTGCTTGACCTACAACAATGGCAGATTACGGAACTT AATTTCGGATTCTATGAAGGCGAAGATTACTGCATATGTGATCATACTTGCCTTGCACATACATGACTTC CAAATTGACCTGACAGTGTTACAGAGGGACTTGAAGCTCAGTGAGAAAAGGATGATGGAGATAGCCAAAG CCATGAGGCTGAAGATCTCCAAAAGAAAGGTGTCTGTGGCCGCCGGCAGTGAAGAAGATCACAAACTGGG CACCCTGTCCCTCCCGCTGCCTCCAGCCCAGACCTCAGACCGCCTGGCAAAGCGGAGGAAGATTACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204811 protein sequence
Red=Cloning site Green=Tags(s) MAAEVLPSARWQYCGAPDGSQRAVLVQFSNGKLQSPGNMRFTLYENKDSTNPRKRNQRILAAETDRLSYV GNNFGTGALKCNTLCRHFVGILNKTSGQMEVYDAELFNMQPLFSDVSVESELALESQTKTYREKMDSCIE AFGTTKQKRALNTRRMNRVGNESLNRAVAKAAETIIDTKGVTALVSDAIHNDLQDDSLYLPPCYDDAAKP EDVYKFEDLLSPAEYEALQSPSEAFRNVTSEEILKMIEENSHCTFVIEALKSLPSDVESRDRQARCIWFL DTLIKFRAHRVVKRKSALGPGVPHIINTKLLKHFTCLTYNNGRLRNLISDSMKAKITAYVIILALHIHDF QIDLTVLQRDLKLSEKRMMEIAKAMRLKISKRKVSVAAGSEEDHKLGTLSLPLPPAQTSDRLAKRRKIT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_022490 |
ORF Size | 1257 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_022490.1, NM_022490.2, NP_071935.1 |
RefSeq Size | 1884 bp |
RefSeq ORF | 1260 bp |
Locus ID | 64425 |
Protein Families | Transcription Factors |
Protein Pathways | Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase |
MW | 47.2 kDa |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC111400 | POLR1E (untagged)-Human polymerase (RNA) I polypeptide E, 53kDa (POLR1E) |
USD 310.00 |
|
RG204811 | POLR1E (GFP-tagged) - Human polymerase (RNA) I polypeptide E, 53kDa (POLR1E) |
USD 460.00 |
|
RC204811L1 | Lenti ORF clone of Human polymerase (RNA) I polypeptide E, 53kDa (POLR1E), Myc-DDK-tagged |
USD 768.00 |
|
RC204811L2 | Lenti ORF clone of Human polymerase (RNA) I polypeptide E, 53kDa (POLR1E), mGFP tagged |
USD 620.00 |
|
RC204811L3 | Lenti ORF clone of Human polymerase (RNA) I polypeptide E, 53kDa (POLR1E), Myc-DDK-tagged |
USD 620.00 |
|
RC204811L4 | Lenti ORF clone of Human polymerase (RNA) I polypeptide E, 53kDa (POLR1E), mGFP tagged |
USD 620.00 |
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