SLC5A6 (NM_021095) Human Tagged ORF Clone

CAT#: RC204865

SLC5A6 (Myc-DDK-tagged)-Human solute carrier family 5 (sodium-dependent vitamin transporter), member 6 (SLC5A6), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY402831) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204865 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC5A6
• Synonyms: SMVT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204865 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGTAGGGGTGAGCACCTCAGCCCCTCTTTCCCCAACCTCGGGCACAAGCGTGGGCATGTCTACCT
  TCTCCATCATGGACTATGTGGTGTTCGTCCTGCTGCTGGTTCTCTCTCTTGCCATTGGGCTCTACCATGC
  TTGTCGTGGCTGGGGCCGGCATACTGTTGGTGAGCTGCTGATGGCGGACCGCAAAATGGGCTGCCTTCCG
  GTGGCACTGTCCCTGCTGGCCACCTTCCAGTCAGCCGTGGCCATCCTGGGTGTGCCGTCAGAGATCTACC
  GATTTGGGACCCAATATTGGTTCCTGGGCTGCTGCTACTTTCTGGGGCTGCTGATACCTGCACACATCTT
  CATCCCCGTTTTCTACCGCCTGCATCTCACCAGTGCCTATGAGTACCTGGAGCTTCGATTCAATAAAACT
  GTGCGAGTGTGTGGAACTGTGACCTTCATCTTTCAGATGGTGATCTACATGGGAGTTGTGCTCTATGCTC
  CGTCATTGGCTCTCAATGCAGTGACTGGCTTTGATCTGTGGCTGTCCGTGCTGGCCCTGGGCATTGTCTG
  TACCGTCTATACAGCTCTGGGTGGGCTGAAGGCCGTCATCTGGACAGATGTGTTCCAGACACTGGTCATG
  TTCCTCGGGCAGCTGGCAGTTATCATCGTGGGGTCAGCCAAGGTGGGCGGCTTGGGGCGTGTGTGGGCCG
  TGGCTTCCCAGCACGGCCGCATCTCTGGGTTTGAGCTGGATCCAGACCCCTTTGTGCGGCACACCTTCTG
  GACCTTGGCCTTCGGGGGTGTCTTCATGATGCTCTCCTTATACGGGGTGAACCAGGCTCAGGTGCAGCGG
  TACCTCAGTTCCCGCACGGAGAAGGCTGCTGTGCTCTCCTGTTATGCAGTGTTCCCCTTCCAGCAGGTGT
  CCCTCTGCGTGGGCTGCCTCATTGGCCTGGTCATGTTCGCGTATTACCAGGAGTATCCCATGAGCATTCA
  GCAGGCTCAGGCAGCCCCAGACCAGTTCGTCCTGTACTTTGTGATGGATCTCCTGAAGGGCCTGCCAGGC
  CTGCCAGGGCTCTTCATTGCCTGCCTCTTCAGCGGCTCTCTCAGCACTATATCCTCTGCTTTTAATTCAT
  TGGCAACTGTTACGATGGAAGACCTGATTCGACCTTGGTTCCCTGAGTTCTCTGAAGCCCGGGCCATCAT
  GCTTTCCAGAGGCCTTGCCTTTGGCTATGGGCTGCTTTGTCTAGGAATGGCCTATATTTCCTCCCAGATG
  GGACCTGTGCTGCAGGCAGCAATCAGCATCTTTGGCATGGTTGGGGGACCGCTGCTGGGACTCTTCTGCC
  TTGGAATGTTCTTTCCATGTGCTAACCCTCCTGGTGCTGTTGTGGGCCTGTTGGCTGGGCTCGTCATGGC
  CTTCTGGATTGGCATCGGGAGCATCGTGACCAGCATGGGCTCCAGCATGCCACCCTCTCCCTCTAATGGG
  TCCAGCTTCTCCCTGCCCACCAATCTAACCGTTGCCACTGTGACCACACTGATGCCCTTGACTACCTTCT
  CCAAGCCCACAGGGCTGCAGCGGTTCTATTCCTTGTCTTACTTATGGTACAGTGCTCACAACTCCACCAC
  AGTGATTGTGGTGGGCCTGATTGTCAGTCTACTCACTGGGAGAATGCGAGGCCGGTCCCTGAACCCTGCA
  ACCATTTACCCAGTGTTGCCAAAGCTCCTGTCCCTCCTTCCGTTGTCCTGTCAGAAGCGGCTCCACTGCA
  GGAGCTACGGCCAGGACCACCTCGACACTGGCCTGTTTCCTGAGAAGCCGAGGAATGGTGTGCTGGGGGA
  CAGCAGAGACAAGGAGGCCATGGCCCTGGATGGCACAGCCTATCAGGGGAGCAGCTCCACCTGCATCCTC
  CAGGAGACCTCCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204865 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSVGVSTSAPLSPTSGTSVGMSTFSIMDYVVFVLLLVLSLAIGLYHACRGWGRHTVGELLMADRKMGCLP
  VALSLLATFQSAVAILGVPSEIYRFGTQYWFLGCCYFLGLLIPAHIFIPVFYRLHLTSAYEYLELRFNKT
  VRVCGTVTFIFQMVIYMGVVLYAPSLALNAVTGFDLWLSVLALGIVCTVYTALGGLKAVIWTDVFQTLVM
  FLGQLAVIIVGSAKVGGLGRVWAVASQHGRISGFELDPDPFVRHTFWTLAFGGVFMMLSLYGVNQAQVQR
  YLSSRTEKAAVLSCYAVFPFQQVSLCVGCLIGLVMFAYYQEYPMSIQQAQAAPDQFVLYFVMDLLKGLPG
  LPGLFIACLFSGSLSTISSAFNSLATVTMEDLIRPWFPEFSEARAIMLSRGLAFGYGLLCLGMAYISSQM
  GPVLQAAISIFGMVGGPLLGLFCLGMFFPCANPPGAVVGLLAGLVMAFWIGIGSIVTSMGSSMPPSPSNG
  SSFSLPTNLTVATVTTLMPLTTFSKPTGLQRFYSLSYLWYSAHNSTTVIVVGLIVSLLTGRMRGRSLNPA
  TIYPVLPKLLSLLPLSCQKRLHCRSYGQDHLDTGLFPEKPRNGVLGDSRDKEAMALDGTAYQGSSSTCIL
  QETSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021095
• ORF Size: 1905 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021095.1, NM_021095.2, NP_066918.1
• RefSeq Size: 3272 bp
• RefSeq ORF: 1908 bp
• Locus ID: 8884
• Domains: SSF
• Protein Families: Transmembrane
• MW: 68.6 kDa
• Gene Summary: Transports pantothenate, biotin and lipoate in the presence of sodium. [UniProtKB/Swiss-Prot Function]