AMID (AIFM2) (NM_032797) Human Tagged ORF Clone

CAT#: RC204934

AIFM2 (Myc-DDK-tagged)-Human apoptosis-inducing factor, mitochondrion-associated, 2 (AIFM2), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY403212) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204934 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified AIFM2 protein (Cat# TP304934). The protein was produced from HEK293T cells transfected with AIFM2 cDNA clone (Cat# RC204934) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AIFM2
• Synonyms: AMID; PRG3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204934 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTCCCAGGTCTCGGTGGAATCGGGAGCTCTGCACGTGGTGATTGTGGGTGGGGGCTTTGGCGGGA
  TCGCAGCAGCCAGCCAGCTGCAGGCCCTGAACGTCCCCTTCATGCTGGTGGACATGAAGGACTCCTTCCA
  CCACAATGTGGCTGCTCTCCGAGCCTCCGTGGAGACAGGGTTCGCCAAAAAGACATTCATTTCTTACTCG
  GTGACTTTCAAGGACAACTTCCGGCAGGGGCTAGTAGTGGGGATAGACCTGAAGAACCAGATGGTGCTGC
  TGCAGGGTGGCGAGGCCCTGCCCTTCTCTCATCTTATCCTGGCCACGGGCAGCACTGGGCCCTTCCCGGG
  CAAGTTTAATGAGGTTTCCAGCCAGCAGGCCGCTATCCAGGCCTATGAGGACATGGTGAGGCAGGTCCAG
  CGCTCACGGTTCATCGTGGTGGTGGGAGGAGGCTCGGCTGGAGTGGAGATGGCAGCAGAGATTAAAACAG
  AATATCCTGAGAAAGAGGTCACTCTCATTCACTCCCAAGTGGCCCTGGCTGACAAGGAGCTCCTGCCCTC
  CGTCCGGCAGGAAGTGAAGGAGATCCTCCTCCGGAAGGGCGTGCAGCTGCTGCTGAGTGAGCGGGTGAGC
  AATCTGGAGGAGCTGCCTCTCAATGAGTATCGAGAGTACATCAAAGTGCAGACGGACAAAGGCACAGAGG
  TGGCCACCAACCTGGTGATTCTCTGCACCGGCATCAAGATCAACAGCTCCGCCTACCGCAAAGCGTTTGA
  GAGCAGACTAGCCAGCAGTGGTGCTCTGAGAGTGAACGAGCACCTCCAGGTGGAGGGCCACAGCAACGTC
  TACGCCATTGGTGACTGTGCCGACGTGAGGACGCCCAAGATGGCCTATCTTGCCGGCCTCCACGCCAACA
  TCGCCGTGGCCAACATCGTCAACTCTGTGAAGCAGCGGCCTCTCCAGGCCTACAAGCCGGGTGCACTGAC
  GTTCCTCCTGTCCATGGGGAGAAATGATGGTGTGGGCCAAATCAGTGGCTTCTATGTGGGCCGGCTCATG
  GTTCGGCTGACCAAGAGCCGGGACCTGTTCGTCTCTACGAGCTGGAAAACCATGAGGCAGTCTCCACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204934 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGSQVSVESGALHVVIVGGGFGGIAAASQLQALNVPFMLVDMKDSFHHNVAALRASVETGFAKKTFISYS
  VTFKDNFRQGLVVGIDLKNQMVLLQGGEALPFSHLILATGSTGPFPGKFNEVSSQQAAIQAYEDMVRQVQ
  RSRFIVVVGGGSAGVEMAAEIKTEYPEKEVTLIHSQVALADKELLPSVRQEVKEILLRKGVQLLLSERVS
  NLEELPLNEYREYIKVQTDKGTEVATNLVILCTGIKINSSAYRKAFESRLASSGALRVNEHLQVEGHSNV
  YAIGDCADVRTPKMAYLAGLHANIAVANIVNSVKQRPLQAYKPGALTFLLSMGRNDGVGQISGFYVGRLM
  VRLTKSRDLFVSTSWKTMRQSPP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032797
• ORF Size: 1119 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032797.1, NM_032797.2, NM_032797.3, NM_032797.4, NM_032797.5, NP_116186.1
• RefSeq Size: 3253 bp
• RefSeq ORF: 1122 bp
• Locus ID: 84883
• Domains: pyr_redox
• Protein Families: Druggable Genome, Transmembrane
• MW: 40.5 kDa
• Gene Summary: This gene encodes a flavoprotein oxidoreductase that binds single stranded DNA and is thought to contribute to apoptosis in the presence of bacterial and viral DNA. The expression of this gene is also found to be induced by tumor suppressor protein p53 in colon cancer cells. [provided by RefSeq, Nov 2010]