LIAS (NM_006859) Human Tagged ORF Clone
CAT#: RC204939
LIAS (Myc-DDK-tagged)-Human lipoic acid synthetase (LIAS), nuclear gene encoding mitochondrial protein, transcript variant 1
"NM_006859" in other vectors (7)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | LIAS |
Synonyms | HGCLAS; HUSSY-01; LAS; LIP1; LS; PDHLD |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC204939 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCTACGCTGCGGGGATGCAGCCCGCACCCTGGGGCCCCGGGTATTTGGGAGATATTTTTGCAGCC CAGTCAGACCGTTAAGCTCCTTGCCAGATAAAAAAAAGGAACTCCTACAGAATGGACCAGACCTTCAAGA TTTTGTATCTGGTGATCTTGCAGACAGGAGCACCTGGGATGAATATAAAGGAAACCTAAAACGCCAGAAA GGAGAAAGGTTAAGACTACCTCCATGGCTAAAGACAGAGATTCCCATGGGGAAAAATTACAATAAACTGA AAAATACTTTGCGGAATTTAAATCTCCATACAGTATGTGAGGAAGCTCGATGTCCCAATATTGGAGAGTG TTGGGGAGGTGGAGAATATGCCACCGCCACAGCCACGATCATGTTGATGGGTGACACATGTACAAGAGGT TGCAGATTTTGTTCTGTTAAGACTGCAAGAAATCCTCCTCCACTGGATGCCAGTGAGCCCTACAATACTG CAAAGGCAATTGCAGAATGGGGTCTGGATTATGTTGTCCTGACATCTGTGGATCGAGATGATATGCCTGA TGGGGGAGCTGAACACATTGCAAAGACCGTATCATATTTAAAGGAAAGGAATCCAAAAATCCTTGTGGAG TGTCTTACTCCTGATTTTCGAGGTGATCTCAAAGCAATAGAAAAAGTTGCTCTGTCAGGATTAGATGTGT ATGCACATAATGTAGAAACAGTCCCGGAATTACAGAGTAAGGTTCGTGATCCTCGGGTCAATTTTGATCA GTCCCTACGTGTACTGAAACATGCCAAGAAGGTTCAGCCTGATGTTATTTCTAAAACATCTATAATGTTG GGTTTAGGCGAGAATGATGAGCAAGTATATGCAACAATGAAAGCACTTCGTGAGGCAGATGTAGACTGCT TGACTTTAGGACAATATATGCAGCCAACAAGGCGTCACCTTAAGGTTGAAGAATATATTACTCCTGAAAA ATTCAAATACTGGGAAAAAGTAGGAAATGAACTTGGATTTCATTATACTGCAAGTGGCCCTTTGGTGCGT TCTTCATATAAAGCAGGTGAATTTTTCCTGAAAAATCTAGTGGCTAAAAGAAAAACAAAAGACCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204939 protein sequence
Red=Cloning site Green=Tags(s) MSLRCGDAARTLGPRVFGRYFCSPVRPLSSLPDKKKELLQNGPDLQDFVSGDLADRSTWDEYKGNLKRQK GERLRLPPWLKTEIPMGKNYNKLKNTLRNLNLHTVCEEARCPNIGECWGGGEYATATATIMLMGDTCTRG CRFCSVKTARNPPPLDASEPYNTAKAIAEWGLDYVVLTSVDRDDMPDGGAEHIAKTVSYLKERNPKILVE CLTPDFRGDLKAIEKVALSGLDVYAHNVETVPELQSKVRDPRVNFDQSLRVLKHAKKVQPDVISKTSIML GLGENDEQVYATMKALREADVDCLTLGQYMQPTRRHLKVEEYITPEKFKYWEKVGNELGFHYTASGPLVR SSYKAGEFFLKNLVAKRKTKDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_006859 |
ORF Size | 1116 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_006859.1, NM_006859.2, NM_006859.3, NP_006850.2 |
RefSeq Size | 1764 bp |
RefSeq ORF | 1119 bp |
Locus ID | 11019 |
Protein Pathways | Lipoic acid metabolism, Metabolic pathways |
MW | 41.9 kDa |
Gene Summary | The protein encoded by this gene belongs to the biotin and lipoic acid synthetases family. It localizes in mitochondrion and plays an important role in alpha-(+)-lipoic acid synthesis. It may also function in the sulfur insertion chemistry in lipoate biosynthesis. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC111760 | LIAS (untagged)-Human lipoic acid synthetase (LIAS), nuclear gene encoding mitochondrial protein, transcript variant 1 |
USD 760.00 |
|
SC324499 | LIAS (untagged)-Human lipoic acid synthetase (LIAS), nuclear gene encoding mitochondrial protein, transcript variant 1 |
USD 760.00 |
|
RG204939 | LIAS (GFP-tagged) - Human lipoic acid synthetase (LIAS), nuclear gene encoding mitochondrial protein, transcript variant 1 |
USD 460.00 |
|
RC204939L1 | Lenti ORF clone of Human lipoic acid synthetase (LIAS), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
USD 768.00 |
|
RC204939L2 | Lenti ORF clone of Human lipoic acid synthetase (LIAS), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
USD 620.00 |
|
RC204939L3 | Lenti ORF clone of Human lipoic acid synthetase (LIAS), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC204939L4 | Lenti ORF clone of Human lipoic acid synthetase (LIAS), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
USD 620.00 |
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