SEC11A (NM_014300) Human Tagged ORF Clone
CAT#: RC204971
SEC11A (Myc-DDK-tagged)-Human SEC11 homolog A (S. cerevisiae) (SEC11A)
"NM_014300" in other vectors (7)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | SEC11A |
Synonyms | 1810012E07Rik; SEC11L1; sid2895; SPC18; SPCS4A |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC204971 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTCTCTAGACTTTTTGGACGATGTGCGGCGGATGAACAAGCGGCAGCTCTATTATCAAGTCCTAA ATTTTGGAATGATTGTCTCATCGGCACTAATGATCTGGAAGGGGTTAATGGTAATAACTGGAAGTGAAAG TCCGATTGTAGTGGTGCTCAGTGGCAGCATGGAACCTGCATTTCATAGAGGAGATCTTCTCTTTCTAACA AATCGAGTTGAAGATCCCATACGAGTGGGAGAAATTGTTGTTTTTAGGATAGAAGGAAGAGAGATTCCTA TAGTTCACCGAGTCTTGAAGATTCATGAAAAGCAAAATGGGCATATCAAGTTTTTGACCAAAGGAGATAA TAATGCGGTTGATGACCGAGGCCTCTATAAACAAGGACAACATTGGCTAGAGAAAAAAGATGTTGTGGGG AGAGCCAGGGGATTTGTTCCTTATATTGGAATTGTGACGATCCTCATGAATGACTATCCTAAATTTAAGT ATGCAGTTCTCTTTTTGCTGGGTTTATTCGTGCTGGTTCATCGTGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204971 protein sequence
Red=Cloning site Green=Tags(s) MLSLDFLDDVRRMNKRQLYYQVLNFGMIVSSALMIWKGLMVITGSESPIVVVLSGSMEPAFHRGDLLFLT NRVEDPIRVGEIVVFRIEGREIPIVHRVLKIHEKQNGHIKFLTKGDNNAVDDRGLYKQGQHWLEKKDVVG RARGFVPYIGIVTILMNDYPKFKYAVLFLLGLFVLVHRE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_014300 |
ORF Size | 537 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014300.1, NM_014300.2, NM_014300.3, NP_055115.1 |
RefSeq Size | 1424 bp |
RefSeq ORF | 540 bp |
Locus ID | 23478 |
Domains | Peptidase_S26 |
Protein Families | Druggable Genome, Protease, Transmembrane |
Protein Pathways | Protein export |
MW | 20.6 kDa |
Gene Summary | This gene encodes a member of the peptidase S26B family. The encoded protein is an 18kDa subunit of the signal peptidase complex and has been linked to cell migration and invasion, gastric cancer and lymph node metastasis. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8. [provided by RefSeq, Dec 2012] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC115102 | SEC11A (untagged)-Human SEC11 homolog A (S. cerevisiae) (SEC11A) |
USD 310.00 |
|
SC320451 | SEC11A (untagged)-Human SEC11 homolog A (S. cerevisiae) (SEC11A) |
USD 310.00 |
|
RG204971 | SEC11A (GFP-tagged) - Human SEC11 homolog A (S. cerevisiae) (SEC11A) |
USD 460.00 |
|
RC204971L1 | Lenti ORF clone of Human SEC11 homolog A (S. cerevisiae) (SEC11A), Myc-DDK-tagged |
USD 620.00 |
|
RC204971L2 | Lenti ORF clone of Human SEC11 homolog A (S. cerevisiae) (SEC11A), mGFP tagged |
USD 620.00 |
|
RC204971L3 | Lenti ORF clone of Human SEC11 homolog A (S. cerevisiae) (SEC11A), Myc-DDK-tagged |
USD 620.00 |
|
RC204971L4 | Lenti ORF clone of Human SEC11 homolog A (S. cerevisiae) (SEC11A), mGFP tagged |
USD 620.00 |
{0} Product Review(s)
Be the first one to submit a review