HOXD8 (NM_019558) Human Tagged ORF Clone

CAT#: RC205062

HOXD8 (Myc-DDK-tagged)-Human homeobox D8 (HOXD8), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY412699) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205062 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HOXD8
• Synonyms: HOX4; HOX4E; HOX5.4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205062 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCGTACTTCGTGAACCCGCTGTACTCCAAGTACAAGGCGGCGGCTGCGGCGGCGGCGGCGGCGG
  GCGAGGCCATCAATCCCACTTACTACGACTGTCACTTCGCGCCCGAGGTCGGCGGCCGTCACGCCGCCGC
  CGCAGCAGCCCTGCAGCTCTATGGCAACAGCGCCGCCGGCTTCCCGCACGCGCCCCCGCAGGCGCACGCG
  CACCCGCACCCGTCCCCGCCGCCCTCCGGGACTGGGTGCGGCGGTAGGGAAGGCCGGGGCCAGGAGTACT
  TCCACCCCGGCGGGGGCAGCCCGGCCGCTGCCTACCAGGCCGCCCCCCCTCCTCCTCCGCATCCTCCGCC
  TCCGCCGCCACCTCCCCCCTGCGGCGGGATTGCCTGTCACGGGGAGCCCGCGAAGTTTTACGGATACGAT
  AACTTACAGAGACAGCCGATTTTTACGACCCAGCAAGAGGCCGAGCTGGTACAATATCCTGACTGTAAAT
  CGTCCAGTGGTAATATTGGCGAGGACCCAGACCACTTAAATCAGAGCTCGTCTCCTTCTCAAATGTTTCC
  GTGGATGAGACCACAAGCTCCTGGTAGACGAAGAGGAAGACAAACCTACAGTCGCTTCCAAACTCTAGAG
  TTGGAAAAGGAATTTCTTTTTAACCCCTATCTGACCAGGAAAAGAAGAATCGAGGTTTCCCACGCCCTAG
  CCCTCACCGAGAGACAGGTAAAAATCTGGTTCCAGAACAGGAGAATGAAATGGAAAAAGGAAAACAACAA
  GGACAAATTTCCCGTTTCCCGGCAGGAGGTGAAGGACGGGGAAACGAAAAAGGAAGCCCAAGAGCTGGAG
  GAAGACAGAGCCGAAGGCCTGACAAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205062 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSYFVNPLYSKYKAAAAAAAAAGEAINPTYYDCHFAPEVGGRHAAAAAALQLYGNSAAGFPHAPPQAHA
  HPHPSPPPSGTGCGGREGRGQEYFHPGGGSPAAAYQAAPPPPPHPPPPPPPPPCGGIACHGEPAKFYGYD
  NLQRQPIFTTQQEAELVQYPDCKSSSGNIGEDPDHLNQSSSPSQMFPWMRPQAPGRRRGRQTYSRFQTLE
  LEKEFLFNPYLTRKRRIEVSHALALTERQVKIWFQNRRMKWKKENNKDKFPVSRQEVKDGETKKEAQELE
  EDRAEGLTN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019558
• ORF Size: 597 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019558.1, NM_019558.2, NM_019558.3, NP_062458.1
• RefSeq Size: 2599 bp
• RefSeq ORF: 873 bp
• Locus ID: 3234
• Cytogenetics: 2q31.1
• Domains: homeobox
• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• MW: 31.8 kDa
• Gene Summary: 'This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXD genes located in a cluster on chromosome 2. Deletions that remove the entire HOXD gene cluster or the 5' end of this cluster have been associated with severe limb and genital abnormalities. In addition to effects during embryogenesis, this particular gene may also play a role in adult urogenital tract function. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Dec 2010]'