DDX3Y (NM_004660) Human Tagged ORF Clone

CAT#: RC205164L4

Lenti-ORF clone of DDX3Y (mGFP-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked (DDX3Y), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: mGFP
• Symbol: DDX3Y
• Synonyms: DBY
• Vector: pLenti-C-mGFP-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC205164).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_004660
• ORF Size: 1980 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004660.3, NP_004651.2
• RefSeq Size: 4488
• RefSeq ORF: 1983
• Locus ID: 8653
• Domains: DEAD, helicase_C
• Protein Pathways: RIG-I-like receptor signaling pathway
• MW: 73.2 kDa
• Gene Summary: The protein encoded by this gene is a member of the DEAD-box RNA helicase family, characterized by nine conserved motifs, included the conserved Asp-Glu-Ala-Asp (DEAD) motif. These motifs are thought to be involved in ATP binding, hydrolysis, RNA binding, and in the formation of intramolecular interactions. This protein shares high similarity to DDX3X, on the X chromosome, but a deletion of this gene is not complemented by DDX3X. Mutations in this gene result in male infertility, a reduction in germ cell numbers, and can result in Sertoli-cell only sydrome. Pseudogenes sharing similarity to both this gene and the DDX3X paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]