KIFAP3 (NM_014970) Human Tagged ORF Clone

CAT#: RC205208

KIFAP3 (Myc-DDK-tagged)-Human kinesin-associated protein 3 (KIFAP3), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KIFAP3
• Synonyms: dJ190I16.1; FLA3; KAP-1; KAP-3; KAP3; SMAP; Smg-GDS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205208 representing NM_014970
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAGGGGAGGACGCCAGATACCTCAAAAGGAAAGTTAAAGGAGGGAATATAGATGTACATCCATCAG
  AAAAAGCACTCATTGTTCACTATGAAGTGGAAGCTACCATTCTTGGAGAAATGGGGGACCCCATGTTGGG
  AGAACGAAAAGAATGTCAAAAAATCATTCGACTTAAGAGTCTCAATGCCAACACAGATATAACTTCCCTG
  GCAAGGAAGGTGGTTGAAGAATGTAAACTCATTCATCCTTCAAAACTAAATGAGGTAGAACAGCTGTTGT
  ACTATCTACAGAACCGCCGTGATTCATTGTCAGGAAAAGAGAAAAAAGAAAAATCAAGCAAGCCTAAAGA
  TCCACCTCCTTTTGAAGGAATGGAGATTGATGAAGTTGCTAACATTAATGACATGGATGAATATATTGAG
  TTATTATATGAAGATATTCCTGACAAAGTTCGGGGTTCTGCTTTGATCCTGCAGCTTGCTCGAAATCCTG
  ATAACTTGGAAGAACTACTATTGAATGAAACTGCCCTTGGTGCATTAGCAAGGGTCCTGAGAGAAGACTG
  GAAGCAAAGTGTCGAGTTAGCTACAAACATAATTTACATCTTTTTTTGTTTCTCCAGCTTTTCTCAATTT
  CATGGACTTATTACTCACTATAAAATTGGAGCTCTGTGTATGAATATTATTGATCATGAGTTAAAAAGAC
  ATGAGCTTTGGCAAGAAGAACTCTCAAAGAAGAAGAAAGCTGTTGATGAAGACCCTGAAAACCAAACCTT
  GAGAAAGGATTATGAAAAAACCTTTAAAAAGTACCAGGGGCTTGTGGTAAAACAGGAACAGCTATTACGA
  GTTGCTCTTTATTTGCTTCTGAATCTTGCTGAGGATACTCGTACCGAACTGAAAATGAGGAACAAGAACA
  TAGTTCACATGTTGGTGAAAGCCCTTGATCGGGACAATTTTGAGCTGCTAATTTTAGTTGTGTCATTCTT
  GAAGAAACTCAGCATTTTTATGGAGAATAAAAATGATATGGTGGAAATGGATATTGTTGAAAAACTGGTG
  AAAATGATACCTTGTGAGCATGAAGACCTGCTGAATATCACCCTCCGACTTTTACTAAACCTATCCTTTG
  ACACAGGACTGAGGAATAAGATGGTACAAGTTGGACTGCTTCCCAAGCTCACTGCACTCCTAGGCAATGA
  CAACTACAAACAAATAGCAATGTGTGTTCTTTACCACATAAGCATGGATGACCGCTTTAAATCAATGTTT
  GCATACACTGACTGTATACCACAGTTAATGAAGATGCTGTTTGAATGTTCAGATGAACGAATTGACTTGG
  AACTCATTTCTTTCTGCATTAATCTTGCTGCTAACAAAAGAAATGTACAGCTTATCTGTGAAGGAAATGG
  GCTGAAGATGCTCATGAAGAGGGCTCTGAAGTTTAAGGATCCATTGCTGATGAAAATGATTAGAAACATT
  TCTCAGCATGATGGACCAACTAAAAATCTGTTTATTGATTATGTTGGGGACCTTGCAGCCCAGATCTCTA
  ATGATGAAGAAGAGGAGTTTGTGATTGAATGTTTGGGAACTCTTGCAAACTTGACCATTCCAGACTTAGA
  CTGGGAATTGGTTCTTAAAGAATATAAGTTGGTTCCATACCTCAAGGATAAACTAAAACCAGGTGCTGCA
  GAAGATGATCTTGTTTTAGAAGTGGTTATAATGATTGGAACTGTATCCATGGATGACTCTTGTGCTGCAT
  TGCTAGCCAAATCTGGCATAATCCCTGCACTCATTGAATTGCTAAATGCTCAACAAGAAGATGATGAATT
  TGTGTGTCAGATAATTTATGTCTTCTACCAGATGGTTTTCCACCAAGCCACAAGAGACGTCATAATCAAG
  GAAACACAGGCTCCAGCATATCTCATAGACCTAATGCATGATAAGAATAATGAAATCCGAAAGGTCTGTG
  ATAATACATTAGATATTATAGCGGAATATGATGAAGAATGGGCTAAGAAAATTCAGAGTGAAAAGTTTCG
  CTGGCATAACTCTCAGTGGCTGGAGATGGTAGAGAGTCGTCAGATGGATGAGAGTGAGCAGTACTTGTAT
  GGTGATGATCGAATTGAGCCATACATTCATGAAGGAGATATTCTCGAAAGACCTGACCTTTTCTACAACT
  CAGATGGATTAATTGCCTCTGAAGGAGCCATAAGTCCCGATTTCTTCAATGATTACCACCTTCAAAATGG
  AGATGTTGTTGGGCAGCATTCATTTCCTGGCAGCCTTGGAATGGATGGCTTTGGCCAACCAGTTGGCATT
  CTTGGACGCCCTGCCACAGCATATGGATTCCGCCCTGATGAACCTTACTACTATGGCTATGGATCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205208 representing NM_014970
  Red=Cloning site Green=Tags(s)
  
  MQGEDARYLKRKVKGGNIDVHPSEKALIVHYEVEATILGEMGDPMLGERKECQKIIRLKSLNANTDITSL
  ARKVVEECKLIHPSKLNEVEQLLYYLQNRRDSLSGKEKKEKSSKPKDPPPFEGMEIDEVANINDMDEYIE
  LLYEDIPDKVRGSALILQLARNPDNLEELLLNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSQF
  HGLITHYKIGALCMNIIDHELKRHELWQEELSKKKKAVDEDPENQTLRKDYEKTFKKYQGLVVKQEQLLR
  VALYLLLNLAEDTRTELKMRNKNIVHMLVKALDRDNFELLILVVSFLKKLSIFMENKNDMVEMDIVEKLV
  KMIPCEHEDLLNITLRLLLNLSFDTGLRNKMVQVGLLPKLTALLGNDNYKQIAMCVLYHISMDDRFKSMF
  AYTDCIPQLMKMLFECSDERIDLELISFCINLAANKRNVQLICEGNGLKMLMKRALKFKDPLLMKMIRNI
  SQHDGPTKNLFIDYVGDLAAQISNDEEEEFVIECLGTLANLTIPDLDWELVLKEYKLVPYLKDKLKPGAA
  EDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIELLNAQQEDDEFVCQIIYVFYQMVFHQATRDVIIK
  ETQAPAYLIDLMHDKNNEIRKVCDNTLDIIAEYDEEWAKKIQSEKFRWHNSQWLEMVESRQMDESEQYLY
  GDDRIEPYIHEGDILERPDLFYNSDGLIASEGAISPDFFNDYHLQNGDVVGQHSFPGSLGMDGFGQPVGI
  LGRPATAYGFRPDEPYYYGYGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014970
• ORF Size: 2376 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_014970.1, NM_014970.2, NM_014970.3, NP_055785.2
• RefSeq Size: 3011
• RefSeq ORF: 2379
• Locus ID: 22920
• Domains: Armadillo_seg
• Protein Families: Druggable Genome
• MW: 91.2 kDa
• Gene Summary: The small G protein GDP dissociation stimulator (smg GDS) is a regulator protein having two activities on a group of small G proteins including the Rho and Rap1 family members and Ki-Ras; one is to stimulate their GDP/GTP exchange reactions, and the other is to inhibit their interactions with membranes. The protein encoded by this gene contains 9 'Armadillo' repeats and interacts with the smg GDS protein through these repeats. This protein, which is highly concentrated around the endoplasmic reticulum, is phosphorylated by v-src, and this phosphorylation reduces the affinity of the protein for smg GDS. It is thought that this protein serves as a linker between human chromosome-associated polypeptide (HCAP) and KIF3A/B, a kinesin superfamily protein in the nucleus, and that it plays a role in the interaction of chromosomes with an ATPase motor protein. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]