CRHBP (NM_001882) Human Tagged ORF Clone

CAT#: RC205258

CRHBP (Myc-DDK-tagged)-Human corticotropin releasing hormone binding protein (CRHBP)

Product Images

Western blot validation of overexpression lysate (Cat# LY419679) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205258 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CRHBP protein (Cat# TP305258). The protein was produced from HEK293T cells transfected with CRHBP cDNA clone (Cat# RC205258) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CRHBP
• Synonyms: CRF-BP; CRFBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205258 representing NM_001882
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCCCAACTTCAAACTTCAGTGTCACTTCATTCTCATCTTCCTGACGGCTCTAAGAGGGGAAAGCC
  GGTACCTAGAGCTGAGGGAAGCGGCGGACTACGATCCTTTCCTGCTCTTCAGCGCCAACCTGAAGCGGGA
  GCTGGCTGGGGAGCAGCCGTACCGCCGCGCTCTGCGGTGCCTGGACATGCTGAGCCTCCAGGGCCAGTTC
  ACCTTCACCGCCGACCGGCCGCAGCTGCACTGCGCAGCCTTCTTCATCAGCGAGCCCGAGGAGTTCATTA
  CCATCCACTACGACCAGGTCTCCATCGACTGTCAGGGCGGCGACTTCCTGAAGGTATTTGATGGTTGGAT
  TCTCAAGGGGGAGAAGTTCCCCAGTTCCCAGGATCATCCTCTCCCCTCAGCTGAGCGGTACATAGATTTC
  TGTGAGAGTGGTCTTAGCAGGAGGAGCATCAGATCTTCCCAGAATGTGGCCATGATCTTCTTCCGAGTCC
  ATGAACCAGGAAATGGATTCACATTAACCATAAAGACAGACCCCAACCTCTTTCCTTGCAATGTCATTTC
  TCAGACTCCAAATGGAAAGTTTACCCTGGTAGTTCCACACCAGCATCGAAACTGCAGCTTCTCCATAATT
  TATCCTGTGGTGATCAAAATATCTGATCTTACCCTGGGACACGTAAATGGTCTTCAGTTAAAGAAATCCT
  CAGCAGGTTGCGAGGGAATAGGAGACTTTGTGGAGCTGCTGGGAGGAACTGGATTGGACCCTTCCAAGAT
  GACGCCTTTAGCTGATCTCTGCTACCCCTTTCATGGCCCGGCCCAGATGAAAGTTGGCTGTGACAACACT
  GTGGTGCGCATGGTCTCCAGTGGAAAACACGTAAATCGTGTGACTTTTGAGTATCGTCAGCTGGAGCCGT
  ACGAGCTGGAAAACCCAAATGGAAACAGTATCGGGGAATTCTGTTTGTCTGGTCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205258 representing NM_001882
  Red=Cloning site Green=Tags(s)
  
  MSPNFKLQCHFILIFLTALRGESRYLELREAADYDPFLLFSANLKRELAGEQPYRRALRCLDMLSLQGQF
  TFTADRPQLHCAAFFISEPEEFITIHYDQVSIDCQGGDFLKVFDGWILKGEKFPSSQDHPLPSAERYIDF
  CESGLSRRSIRSSQNVAMIFFRVHEPGNGFTLTIKTDPNLFPCNVISQTPNGKFTLVVPHQHRNCSFSII
  YPVVIKISDLTLGHVNGLQLKKSSAGCEGIGDFVELLGGTGLDPSKMTPLADLCYPFHGPAQMKVGCDNT
  VVRMVSSGKHVNRVTFEYRQLEPYELENPNGNSIGEFCLSGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001882
• ORF Size: 966 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001882.1, NM_001882.2, NM_001882.3, NP_001873.2
• RefSeq Size: 1854 bp
• RefSeq ORF: 969 bp
• Locus ID: 1393
• Cytogenetics: 5q13.3
• MW: 36 kDa
• Gene Summary: 'Corticotropin-releasing hormone is a potent stimulator of synthesis and secretion of preopiomelanocortin-derived peptides. Although CRH concentrations in the human peripheral circulation are normally low, they increase throughout pregnancy and fall rapidly after parturition. Maternal plasma CRH probably originates from the placenta. Human plasma contains a CRH-binding protein which inactivates CRH and which may prevent inappropriate pituitary-adrenal stimulation in pregnancy. [provided by RefSeq, Jul 2008]'