ABHD2 (NM_007011) Human Tagged ORF Clone

CAT#: RC205260

ABHD2 (Myc-DDK-tagged)-Human abhydrolase domain containing 2 (ABHD2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY402075) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205260 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ABHD2 protein (Cat# TP305260). The protein was produced from HEK293T cells transfected with ABHD2 cDNA clone (Cat# RC205260) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ABHD2
• Synonyms: HS1-2; LABH2; PHPS1-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205260 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGCCATGCTGGAGACTCCCGAACTCCCAGCCGTGTTTGATGGAGTGAAGCTGGCTGCAGTGGCTG
  CTGTGCTGTACGTGATCGTCCGGTGTTTGAACCTGAAGAGCCCCACAGCCCCACCTGACCTCTACTTCCA
  GGACTCGGGGCTCTCACGCTTTCTGCTCAAGTCCTGTCCTCTTCTGACCAAAGAATACATTCCACCGTTG
  ATCTGGGGGAAAAGTGGACACATCCAGACAGCCTTGTATGGGAAGATGGGAAGGGTGAGGTCGCCACATC
  CTTATGGGCACCGGAAGTTCATCACTATGTCTGATGGAGCCACTTCTACATTCGACCTCTTCGAGCCCTT
  GGCTGAGCACTGTGTTGGAGATGATATCACCATGGTCATCTGCCCTGGAATTGCCAATCACAGCGAGAAG
  CAATACATCCGCACTTTCGTTGACTACGCCCAGAAAAATGGCTATCGGTGCGCCGTGCTGAACCACCTGG
  GTGCCCTGCCCAACATTGAATTGACCTCGCCACGCATGTTCACCTATGGCTGCACGTGGGAATTTGGAGC
  CATGGTGAACTACATCAAGAAGACATATCCCCTGACCCAGCTGGTCGTCGTGGGCTTCAGCCTGGGTGGT
  AACATTGTGTGCAAATACTTGGGGGAGACTCAGGCAAACCAAGAGAAGGTCCTGTGCTGCGTCAGCGTGT
  GCCAGGGGTACAGTGCACTGAGGGCCCAGGAAACCTTCATGCAATGGGATCAGTGCCAGCGGTTCTACAA
  CTTCCTCATGGCTGACAACATGAAGAAGATCATCCTCTCGCACAGGCAAGCTCTTTTTGGAGACCATGTT
  AAGAAACCCCAGAGCCTGGAAGACACGGACTTGAGCCGGCTCTACACAGCAACATCCCTGATGCAGATTG
  ATGACAATGTGATGAGGAAGTTTCACGGCTATAACTCCCTGAAGGAATACTATGAGGAAGAAAGTTGCAT
  GCGGTACCTGCACAGGATTTATGTTCCTCTCATGCTGGTTAATGCAGCTGACGATCCGTTGGTGCATGAA
  AGTCTTCTAACCATTCCAAAATCTCTTTCAGAGAAACGAGAGAACGTCATGTTTGTGCTGCCTCTGCATG
  GGGGCCACTTGGGCTTCTTTGAGGGCTCTGTGCTGTTCCCCGAGCCCCTGACATGGATGGATAAGCTGGT
  GGTGGAGTACGCCAACGCCATTTGCCAATGGGAGCGTAACAAGTTGCAGTGCTCTGACACGGAGCAGGTG
  GAGGCCGACCTGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205260 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNAMLETPELPAVFDGVKLAAVAAVLYVIVRCLNLKSPTAPPDLYFQDSGLSRFLLKSCPLLTKEYIPPL
  IWGKSGHIQTALYGKMGRVRSPHPYGHRKFITMSDGATSTFDLFEPLAEHCVGDDITMVICPGIANHSEK
  QYIRTFVDYAQKNGYRCAVLNHLGALPNIELTSPRMFTYGCTWEFGAMVNYIKKTYPLTQLVVVGFSLGG
  NIVCKYLGETQANQEKVLCCVSVCQGYSALRAQETFMQWDQCQRFYNFLMADNMKKIILSHRQALFGDHV
  KKPQSLEDTDLSRLYTATSLMQIDDNVMRKFHGYNSLKEYYEEESCMRYLHRIYVPLMLVNAADDPLVHE
  SLLTIPKSLSEKRENVMFVLPLHGGHLGFFEGSVLFPEPLTWMDKLVVEYANAICQWERNKLQCSDTEQV
  EADLE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007011
• ORF Size: 1275 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007011.1, NM_007011.2, NM_007011.3, NM_007011.4, NM_007011.5, NM_007011.6, NM_007011.7, NP_008942.3
• RefSeq Size: 9159 bp
• RefSeq ORF: 1278 bp
• Locus ID: 11057
• Domains: abhydrolase
• MW: 48.3 kDa
• Gene Summary: This gene encodes a protein containing an alpha/beta hydrolase fold, which is a catalytic domain found in a wide range of enzymes. The encoded protein is an acylglycerol lipase that catalyzes the hydrolysis of endocannabinoid arachidonoylglycerol from the cell membrane. This leads to activation of the sperm calcium channel CatSper, which results in sperm activation. Alternative splicing of this gene results in two transcript variants encoding the same protein. [provided by RefSeq, Jan 2017]