ITPKA (NM_002220) Human Tagged ORF Clone

CAT#: RC205323

ITPKA (Myc-DDK-tagged)-Human inositol-trisphosphate 3-kinase A (ITPKA)

Product Images

Western blot validation of overexpression lysate (Cat# LY419458) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205323 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ITPKA protein (Cat# TP305323). The protein was produced from HEK293T cells transfected with ITPKA cDNA clone (Cat# RC205323) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ITPKA
• Synonyms: IP3-3KA; IP3KA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205323 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCTGCCCGGGGGCCCAACGGGCATGGCGCGGCCGGGGGGCGCGAGGCCCTGCAGCCCGGGGCTGG
  AGCGGGCCCCGCGCAGGAGTGTCGGGGAGCTGCGCCTGCTCTTCGAGGCGCGCTGTGCGGCGGTCGCTGC
  GGCCGCCGCCGCGGGGGAGCCCCGGGCCCGCGGGGCCAAGCGGCGTGGGGGACAGGTCCCCAACGGGCTT
  CAGCGGGCTCCCCCGGCCCCGGTGATCCCTCAGCTGACCGTGACAGCCGAGGAGCCCGACGTGCCCCCGA
  CCAGCCCTGGGCCGCCGGAGCGGGAGAGGGACTGCCTCCCGGCAGCGGGCTCTTCGCACCTGCAGCAGCC
  GCGCCGCCTTTCCACCTCGTCGGTCTCCTCCACTGGCTCCTCGTCGCTGCTCGAGGACTCGGAGGACGAC
  CTGCTGAGCGACAGTGAGAGCCGGAGCCGCGGCAACGTGCAGCTGGAAGCGGGCGAGGACGTGGGTCAGA
  AAAACCACTGGCAGAAGATCCGGACCATGGTCAATCTGCCGGTCATAAGCCCTTTCAAGAAGCGCTACGC
  CTGGGTGCAGCTGGCAGGGCACACTGGGAGTTTTAAGGCGGCGGGCACCAGCGGGCTGATCCTGAAGCGC
  TGCTCGGAGCCGGAGCGCTACTGCCTGGCGCGGCTGATGGCTGACGCGCTGCGCGGCTGCGTGCCTGCCT
  TCCACGGCGTGGTGGAGCGCGACGGCGAAAGCTACCTGCAGCTGCAGGACCTGCTCGATGGCTTCGACGG
  ACCTTGTGTGCTCGACTGCAAAATGGGCGTCAGGACTTACCTAGAGGAGGAGCTGACCAAGGCCCGTGAG
  CGGCCCAAGCTGCGGAAGGACATGTACAAGAAAATGCTGGCGGTGGATCCTGAAGCTCCCACGGAGGAGG
  AGCACGCGCAGCGCGCCGTCACCAAGCCGCGCTACATGCAGTGGCGGGAAGGCATCAGCTCCAGCACCAC
  CCTCGGCTTCCGCATCGAGGGCATCAAGAAAGCGGACGGCTCCTGCAGCACCGACTTCAAGACTACGCGA
  AGCCGAGAGCAGGTGCTTCGCGTCTTTGAAGAGTTTGTGCAAGGAGATGAGGAAGTGCTGAGGCGGTATC
  TGAACCGCCTGCAGCAGATCCGGGACACCCTGGAGGTATCCGAGTTCTTCAGGAGGCACGAGGTGATCGG
  CAGCTCGCTCCTCTTTGTGCACGATCACTGCCATCGCGCCGGCGTGTGGCTCATCGACTTCGGCAAGACC
  ACGCCCCTCCCCGATGGCCAGATCCTGGACCACCGGCGGCCCTGGGAGGAGGGCAACCGCGAGGACGGCT
  ATTTGCTGGGGCTGGACAATCTCATTGGCATCCTGGCCAGCCTGGCTGAGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205323 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTLPGGPTGMARPGGARPCSPGLERAPRRSVGELRLLFEARCAAVAAAAAAGEPRARGAKRRGGQVPNGL
  QRAPPAPVIPQLTVTAEEPDVPPTSPGPPERERDCLPAAGSSHLQQPRRLSTSSVSSTGSSSLLEDSEDD
  LLSDSESRSRGNVQLEAGEDVGQKNHWQKIRTMVNLPVISPFKKRYAWVQLAGHTGSFKAAGTSGLILKR
  CSEPERYCLARLMADALRGCVPAFHGVVERDGESYLQLQDLLDGFDGPCVLDCKMGVRTYLEEELTKARE
  RPKLRKDMYKKMLAVDPEAPTEEEHAQRAVTKPRYMQWREGISSSTTLGFRIEGIKKADGSCSTDFKTTR
  SREQVLRVFEEFVQGDEEVLRRYLNRLQQIRDTLEVSEFFRRHEVIGSSLLFVHDHCHRAGVWLIDFGKT
  TPLPDGQILDHRRPWEEGNREDGYLLGLDNLIGILASLAER
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002220
• ORF Size: 1383 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002220.1, NM_002220.2, NP_002211.1
• RefSeq Size: 1864 bp
• RefSeq ORF: 1386 bp
• Locus ID: 3706
• Cytogenetics: 15q15.1
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• MW: 51 kDa
• Gene Summary: 'Regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 1,4,5-trisphosphate to Ins(1,3,4,5)P4. The activity of the inositol 1,4,5-trisphosphate 3-kinase is responsible for regulating the levels of a large number of inositol polyphosphates that are important in cellular signaling. Both calcium/calmodulin and protein phosphorylation mechanisms control its activity. It is also a substrate for the cyclic AMP-dependent protein kinase, calcium/calmodulin- dependent protein kinase II, and protein kinase C in vitro.[provided by RefSeq, Apr 2011]'