ProDynorphin (PDYN) (NM_024411) Human Tagged ORF Clone
CAT#: RC205331
PDYN (Myc-DDK-tagged)-Human prodynorphin (PDYN), transcript variant 1
"NM_024411" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PDYN |
Synonyms | ADCA; PENKB; SCA23 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC205331 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTGGCAGGGGCTGGTCCTGGCTGCCTGCCTCCTCATGTTCCCCTCCACCACAGCGGACTGCCTGT CGCGGTGCTCCTTGTGTGCTGTAAAGACCCAGGATGGTCCCAAACCTATCAATCCCCTGATTTGCTCCCT GCAATGCCAGGCTGCCCTGCTGCCCTCTGAGGAATGGGAGAGATGCCAGAGCTTTCTGTCTTTTTTCACC CCCTCCACCCTTGGGCTCAATGACAAGGAGGACTTGGGGAGCAAGTCGGTTGGGGAAGGGCCCTACAGTG AGCTGGCCAAGCTCTCTGGGTCATTCCTGAAGGAGCTGGAGAAAAGCAAGTTTCTCCCAAGTATCTCAAC AAAGGAGAACACTCTGAGCAAGAGCCTGGAGGAGAAGCTCAGGGGTCTCTCTGACGGGTTTAGGGAGGGA GCAGAGTCTGAGCTGATGAGGGATGCCCAGCTGAACGATGGTGCCATGGAGACTGGCACACTCTATCTCG CTGAGGAGGACCCCAAGGAGCAGGTCAAACGCTATGGGGGCTTTTTGCGCAAATACCCCAAGAGGAGCTC AGAGGTGGCTGGGGAGGGGGACGGGGATAGCATGGGCCATGAGGACCTGTACAAACGCTATGGGGGCTTC TTGCGGCGCATTCGTCCCAAGCTCAAGTGGGACAACCAGAAGCGCTATGGCGGTTTTCTCCGGCGCCAGT TCAAGGTGGTGACTCGGTCTCAGGAAGATCCGAATGCTTACTCTGGAGAGCTTTTTGATGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205331 protein sequence
Red=Cloning site Green=Tags(s) MAWQGLVLAACLLMFPSTTADCLSRCSLCAVKTQDGPKPINPLICSLQCQAALLPSEEWERCQSFLSFFT PSTLGLNDKEDLGSKSVGEGPYSELAKLSGSFLKELEKSKFLPSISTKENTLSKSLEEKLRGLSDGFREG AESELMRDAQLNDGAMETGTLYLAEEDPKEQVKRYGGFLRKYPKRSSEVAGEGDGDSMGHEDLYKRYGGF LRRIRPKLKWDNQKRYGGFLRRQFKVVTRSQEDPNAYSGELFDA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_024411 |
ORF Size | 762 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_024411.1, NM_024411.2, NM_024411.3, NM_024411.4, NP_077722.1 |
RefSeq Size | 2800 bp |
RefSeq ORF | 765 bp |
Locus ID | 5173 |
Cytogenetics | 20p13 |
Domains | Opiods_neuropep |
Protein Families | Secreted Protein |
MW | 28.4 kDa |
Gene Summary | 'The protein encoded by this gene is a preproprotein that is proteolytically processed to form the secreted opioid peptides beta-neoendorphin, dynorphin, leu-enkephalin, rimorphin, and leumorphin. These peptides are ligands for the kappa-type of opioid receptor. Dynorphin is involved in modulating responses to several psychoactive substances, including cocaine. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC112227 | PDYN (untagged)-Human prodynorphin (PDYN), transcript variant 1 |
USD 310.00 |
|
RG205331 | PDYN (GFP-tagged) - Human prodynorphin (PDYN), transcript variant 1 |
USD 460.00 |
|
RC205331L1 | Lenti ORF clone of Human prodynorphin (PDYN), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC205331L2 | Lenti ORF clone of Human prodynorphin (PDYN), transcript variant 1, mGFP tagged |
USD 620.00 |
|
RC205331L3 | Lenti ORF clone of Human prodynorphin (PDYN), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC205331L4 | Lenti ORF clone of Human prodynorphin (PDYN), transcript variant 1, mGFP tagged |
USD 620.00 |
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