PIGN (NM_176787) Human Tagged ORF Clone

CAT#: RC205334

PIGN (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY406118) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205334 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PIGN
• Synonyms: MCAHS; MCAHS1; MCD4; MDC4; PIG-N
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205334 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTGTTCTTTACTTTGGGATTGCTTATACATTTTGTGTTCTTCGCCTCCATCTTTGACATTTATT
  TTACATCTCCTTTGGTTCATGGAATGACTCCTCAGTTTACACCATTGCCTCCTCCAGCGAGAAGATTAGT
  GTTGTTTGTTGCTGATGGCCTTCGAGCAGATGCACTTTACGAATTAGATGAAAATGGAAACTCTAGAGCA
  CCGTTTATTAGGAATATCATAATGCATGAAGGCAGCTGGGGCATATCTCATACACGTGTGCCAACAGAAT
  CTCGGCCAGGTCATGTAGCTCTGATAGCTGGGTTTTATGAAGATGTCAGTGCAGTTGCCAAAGGATGGAA
  GGAAAATCCTGTAGAGTTTGATTCTCTTTTTAATGAAAGTAAATACACATGGAGCTGGGGAAGCCCAGAT
  ATCCTGCCTATGTTTGCCAAAGGTGCTAGTGGAGACCACGTTTATACATATAGTTATGATGCTAAAAGAG
  AGGATTTTGGTGCTCAAGATGCAACAAAACTGGATACGTGGGTTTTTGATAATGTTAAGGACTTCTTTCA
  TCATGCCAGAAACAACCAGTCTTTGTTTTCTAAAATAAATGAAGAGAAAATAGTTTTTTTCTTACATTTA
  TTAGGAATAGATACAAACGGACATGCTCATCGACCATCCTCGAGAGACTACAAGGACAATATTAAAAAAG
  TTGATGATGGAGTTAAAGAAATCGTGTCTATGTTTAACCATTTCTATGGAAATGATGGGAAAACAACATT
  TATCTTTACCTCTGACCATGGAATGACAGACTGGGGTTCCCATGGGGCTGGTCATCCTTCAGAGACTTTA
  ACTCCTTTAGTCACTTGGGGAGCTGGAATCAAGTATCCCCAAAGAGTATCAGCTCAGCAATTTGATGATG
  CATTTTTGAAAGAGTGGAGATTGGAGAATTGGAAGAGGCTAGATGTCAATCAGGCTGATATTGCACCATT
  GATGACTTCCCTTATTGGAGTTCCCTTTCCTCTTAACTCAGTGGGAATCCTTCCTGTGGATTATCTTAAC
  AACACTGATCTCTTCAAAGCAGAGAGCATGTTTACAAATGCAGTACAGATTCTTGAACAGTTCAAGGTGA
  AAATGACTCAGAAGAAAGAAGTTACTTTACCATTTTTGTTTACACCATTTAAACTGCTTTCTGATTCCAA
  ACAGTTCAACATTTTAAGAAAAGCAAGATCTTATATAAAACACAGAAAGTTTGATGAAGTGGTCTCCCTT
  TGCAAGGAGCTAATTCATCTTGCATTGAAAGGATTGTCCTATTATCACACATATGACAGATTCTTTTTGG
  GCGTCAATGTTGTTATTGGTTTTGTGGGATGGATATCTTATGCCTCTTTGTTGATCATCAAGTCTCATTC
  CAACCTTATAAAAGGTGTTAGTAAAGAAGTGAAGAAACCAAGCCATCTCCTGCCTTGTAGTTTTGTAGCT
  ATTGGCATTTTAGTAGCATTTTTTCTGCTGATTCAAGCCTGTCCCTGGACATATTATGTATATGGTTTGT
  TGCCACTGCCAATATGGTATGCGGTTCTAAGAGAATTTCAAGTTATTCAGGACCTTGTTGTATCAGTGTT
  GACCTATCCTCTGAGCCATTTTGTTGGGTACCTGTTAGCCTTTACCCTGGGAATTGAAGTATTAGTTCTC
  AGTTTTTTCTACCGCTATATGCTTACCGCTGGACTTACTGCCTTTGCAGCTTGGCCATTTCTCACTCGGC
  TGTGGACTCGAGCAAAGATGACCTCACTGAGTTGGACTTTCTTCTCTTTGCTCCTGGCAGTGTTCCCACT
  GATGCCGGTTGTAGGTCGAAAGCCAGACATCTCTCTAGTGATGGGTGCAGGCTTGCTGGTTCTTCTGTTA
  TCCCTGTGTGTTGTAACATCTCTCATGAAAAGAAAAGATAGCTTTATAAAGGAAGAGCTATTGGTACATC
  TGTTACAGGTGCTGAGCACAGTGCTCTCCATGTATGTTGTGTATAGCACTCAGAGTAGTCTACTCAGGAA
  GCAAGGACTGCCTCTCATGAATCAAATTATTAGCTGGGCAACATTAGCCTCTTCCTTGGTTGTGCCACTA
  CTGAGTTCTCCAGTTCTCTTTCAGCGATTGTTCAGCATACTTCTTTCATTGATGTCAACCTACCTACTTC
  TAAGCACAGGGTATGAAGCTCTCTTTCCACTAGTGTTGTCTTGTTTGATGTTTGTCTGGATAAACATAGA
  ACAAGAAACTCTACAACAATCTGGTGTTTGCTGTAAACAAAAGCTCACCAGTATCCAGTTCTCTTATAAT
  ACTGATATAACTCAGTTTCGACAGCTATATCTGGATGACATCCGTAGGGCCTTTTTCCTTGTTTTCTTCT
  TAGTGACAGCATTTTTTGGAACTGGAAATATAGCTTCTATTAACAGCTTTGATCTTGCCTCTGTCTATTG
  CTTTCTGACTGTGTTCAGTCCTTTTATGATGGGAGCCCTGATGATGTGGAAGATTTTAATCCCCTTTGTT
  CTTGTTATGTGTGCTTTTGAAGCAGTTCAGTTGACTACTCAGTTATCGTCAAAAAGCCTTTTTCTCATTG
  TTCTCGTCATATCAGACATTATGGCTTTGCATTTTTTCTTCTTGGTCAAGGATTATGGCAGCTGGCTTGA
  TATTGGGACAAGCATCAGCCACTATGTGATTGTCATGTCCATGACCATCTTTTTGGTGTTCCTCAATGGC
  CTGGCCCAGCTGCTCACAACGAAGAAACTCAGACTATGTGGCAAACCCAAAAGTCACTTCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205334 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLLFFTLGLLIHFVFFASIFDIYFTSPLVHGMTPQFTPLPPPARRLVLFVADGLRADALYELDENGNSRA
  PFIRNIIMHEGSWGISHTRVPTESRPGHVALIAGFYEDVSAVAKGWKENPVEFDSLFNESKYTWSWGSPD
  ILPMFAKGASGDHVYTYSYDAKREDFGAQDATKLDTWVFDNVKDFFHHARNNQSLFSKINEEKIVFFLHL
  LGIDTNGHAHRPSSRDYKDNIKKVDDGVKEIVSMFNHFYGNDGKTTFIFTSDHGMTDWGSHGAGHPSETL
  TPLVTWGAGIKYPQRVSAQQFDDAFLKEWRLENWKRLDVNQADIAPLMTSLIGVPFPLNSVGILPVDYLN
  NTDLFKAESMFTNAVQILEQFKVKMTQKKEVTLPFLFTPFKLLSDSKQFNILRKARSYIKHRKFDEVVSL
  CKELIHLALKGLSYYHTYDRFFLGVNVVIGFVGWISYASLLIIKSHSNLIKGVSKEVKKPSHLLPCSFVA
  IGILVAFFLLIQACPWTYYVYGLLPLPIWYAVLREFQVIQDLVVSVLTYPLSHFVGYLLAFTLGIEVLVL
  SFFYRYMLTAGLTAFAAWPFLTRLWTRAKMTSLSWTFFSLLLAVFPLMPVVGRKPDISLVMGAGLLVLLL
  SLCVVTSLMKRKDSFIKEELLVHLLQVLSTVLSMYVVYSTQSSLLRKQGLPLMNQIISWATLASSLVVPL
  LSSPVLFQRLFSILLSLMSTYLLLSTGYEALFPLVLSCLMFVWINIEQETLQQSGVCCKQKLTSIQFSYN
  TDITQFRQLYLDDIRRAFFLVFFLVTAFFGTGNIASINSFDLASVYCFLTVFSPFMMGALMMWKILIPFV
  LVMCAFEAVQLTTQLSSKSLFLIVLVISDIMALHFFFLVKDYGSWLDIGTSISHYVIVMSMTIFLVFLNG
  LAQLLTTKKLRLCGKPKSHFM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_176787
• ORF Size: 2793 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176787.1, NM_176787.2, NM_176787.3, NM_176787.4, NP_789744.1
• RefSeq Size: 4893 bp
• RefSeq ORF: 2796 bp
• Locus ID: 23556
• Protein Families: Transmembrane
• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
• MW: 105.8 kDa
• Gene Summary: This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is expressed in the endoplasmic reticulum and transfers phosphoethanolamine (EtNP) to the first mannose of the GPI anchor. Two alternatively spliced variants, which encode an identical isoform, have been reported. [provided by RefSeq, Jul 2008]