B3GALT2 (NM_003783) Human Tagged ORF Clone

CAT#: RC205366

B3GALT2 (Myc-DDK-tagged)-Human UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 2 (B3GALT2)

Product Images

Western blot validation of overexpression lysate (Cat# LY401245) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205366 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: B3GALT2
• Synonyms: beta3Gal-T2; BETA3GALT2; GLCT2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205366 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCAGTGGAGGAGAAGACACTGCTGCTTTGCAAAGATGACCTGGAATGCCAAAAGGTCTCTGTTCC
  GCACTCATCTTATTGGAGTACTTTCTCTAGTGTTTCTTTTTGCTATGTTTTTGTTTTTCAATCATCATGA
  CTGGCTGCCAGGCAGAGCTGGATTCAAAGAAAACCCTGTGACATACACTTTCCGAGGATTTCGGTCAACA
  AAAAGTGAGACAAACCACAGCTCCCTTCGGAACATTTGGAAAGAAACAGTCCCTCAAACCCTGAGGCCTC
  AAACAGCAACTAACTCTAATAACACAGACCTGTCACCACAAGGAGTTACAGGCCTGGAGAATACACTTAG
  TGCCAATGGAAGTATTTACAATGAAAAAGGTACTGGACATCCAAATTCTTACCATTTCAAATATATTATT
  AATGAGCCTGAAAAATGCCAAGAGAAAAGTCCTTTTTTAATACTACTAATAGCTGCAGAGCCTGGACAAA
  TAGAAGCTAGAAGAGCTATTCGGCAAACTTGGGGCAATGAAAGTCTAGCACCTGGTATTCAAATCACAAG
  AATATTTTTGTTGGGCTTAAGTATTAAGCTAAATGGCTACCTTCAACGTGCAATACTGGAAGAAAGCAGA
  CAATATCATGATATAATTCAACAGGAATACTTAGATACGTACTATAATTTGACCATTAAAACACTAATGG
  GCATGAACTGGGTTGCAACATACTGTCCACATATTCCATATGTTATGAAAACTGACAGTGACATGTTTGT
  CAACACTGAATATTTAATCAATAAGTTACTGAAGCCAGATCTGCCTCCCAGACATAACTATTTCACTGGT
  TACCTAATGCGAGGATATGCACCCAATCGAAACAAAGATAGCAAGTGGTACATGCCACCAGACCTCTACC
  CAAGTGAGCGTTATCCTGTCTTCTGTTCTGGAACTGGTTATGTTTTTTCTGGAGATCTGGCAGAAAAGAT
  TTTTAAAGTTTCTTTAGGTATCCGCCGTTTGCACTTGGAAGATGTATATGTAGGGATCTGTCTTGCCAAG
  TTGAGAATTGATCCTGTACCCCCTCCCAATGAGTTTGTGTTCAATCACTGGCGAGTCTCTTATTCGAGCT
  GTAAATACAGCCACCTAATTACCTCTCATCAGTTCCAGCCTAGTGAACTGATAAAATACTGGAACCATTT
  ACAACAAAATAAGCACAATGCCTGTGCCAACGCAGCAAAAGAAAAGGCAGGCAGGTATCGCCACCGTAAA
  CTACAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205366 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLQWRRRHCCFAKMTWNAKRSLFRTHLIGVLSLVFLFAMFLFFNHHDWLPGRAGFKENPVTYTFRGFRST
  KSETNHSSLRNIWKETVPQTLRPQTATNSNNTDLSPQGVTGLENTLSANGSIYNEKGTGHPNSYHFKYII
  NEPEKCQEKSPFLILLIAAEPGQIEARRAIRQTWGNESLAPGIQITRIFLLGLSIKLNGYLQRAILEESR
  QYHDIIQQEYLDTYYNLTIKTLMGMNWVATYCPHIPYVMKTDSDMFVNTEYLINKLLKPDLPPRHNYFTG
  YLMRGYAPNRNKDSKWYMPPDLYPSERYPVFCSGTGYVFSGDLAEKIFKVSLGIRRLHLEDVYVGICLAK
  LRIDPVPPPNEFVFNHWRVSYSSCKYSHLITSHQFQPSELIKYWNHLQQNKHNACANAAKEKAGRYRHRK
  LH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003783
• ORF Size: 1266 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003783.1, NM_003783.2, NM_003783.3, NP_003774.1
• RefSeq Size: 3548 bp
• RefSeq ORF: 1269 bp
• Locus ID: 8707
• Domains: Galactosyl_T
• Protein Families: Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - lacto and neolacto series, Metabolic pathways
• MW: 49.2 kDa
• Gene Summary: This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). This gene encodes a protein that functions in N-linked glycoprotein glycosylation and shows strict donor substrate specificity for UDP-galactose. [provided by RefSeq, Jul 2008]