VTA1 (NM_016485) Human Tagged ORF Clone

CAT#: RC205442

VTA1 (Myc-DDK-tagged)-Human Vps20-associated 1 homolog (S. cerevisiae) (VTA1)

Product Images

Western blot validation of overexpression lysate (Cat# LY413927) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205442 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified VTA1 protein (Cat# TP305442). The protein was produced from HEK293T cells transfected with VTA1 cDNA clone (Cat# RC205442) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VTA1
• Synonyms: C6orf55; DRG-1; DRG1; HSPC228; LIP5; My012; SBP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205442 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCGCTTGCACCGCTGCCCCCGCTCCCCGCACAGTTCAAGAGCATACAGCATCATCTGAGGACGG
  CTCAGGAGCATGACAAGCGAGACCCTGTGGTGGCTTATTACTGTCGTTTATACGCAATGCAGACTGGAAT
  GAAGATCGATAGTAAAACTCCTGAATGTCGCAAATTTTTATCAAAGTTAATGGATCAGTTAGAAGCTCTA
  AAGAAGCAGTTGGGTGATAATGAAGCTATTACTCAAGAAATAGTGGGCTGTGCCCATTTGGAGAATTATG
  CTTTGAAAATGTTTTTGTATGCAGACAATGAAGATCGTGCTGGACGATTTCACAAAAACATGATCAAGTC
  CTTCTATACTGCAAGTCTTTTGATAGATGTCATAACAGTATTTGGAGAACTCACTGATGAAAATGTGAAA
  CACAGGAAGTATGCCAGATGGAAGGCAACATACATCCATAATTGTTTAAAGAATGGGGAGACTCCTCAAG
  CAGGCCCTGTTGGAATTGAAGAAGATAATGATATTGAAGAAAATGAAGATGCTGGAGCAGCCTCTCTGCC
  CACTCAGCCAACTCAGCCATCATCATCTTCAACTTATGACCCAAGCAACATGCCATCAGGCAACTATACT
  GGAATACAGATTCCTCCGGGTGCACACGCTCCAGCTAATACACCAGCAGAAGTGCCTCACAGCACAGGTG
  TAGCAAGTAATACTATCCAACCTACTCCACAGACTATACCTGCCATTGATCCCGCACTTTTCAATACAAT
  TTCCCAGGGGGATGTTCGTCTAACCCCAGAAGACTTTGCTAGAGCTCAGAAGTACTGCAAATATGCTGGC
  AGTGCTTTGCAGTATGAAGATGTAAGCACTGCTGTCCAGAATCTACAAAAGGCTCTCAAGTTACTGACGA
  CAGGCAGAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205442 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAALAPLPPLPAQFKSIQHHLRTAQEHDKRDPVVAYYCRLYAMQTGMKIDSKTPECRKFLSKLMDQLEAL
  KKQLGDNEAITQEIVGCAHLENYALKMFLYADNEDRAGRFHKNMIKSFYTASLLIDVITVFGELTDENVK
  HRKYARWKATYIHNCLKNGETPQAGPVGIEEDNDIEENEDAGAASLPTQPTQPSSSSTYDPSNMPSGNYT
  GIQIPPGAHAPANTPAEVPHSTGVASNTIQPTPQTIPAIDPALFNTISQGDVRLTPEDFARAQKYCKYAG
  SALQYEDVSTAVQNLQKALKLLTTGRE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016485
• ORF Size: 921 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016485.1, NM_016485.2, NM_016485.3, NM_016485.4, NP_057569.2
• RefSeq Size: 3371 bp
• RefSeq ORF: 924 bp
• Locus ID: 51534
• Domains: DUF605
• Protein Pathways: Endocytosis
• MW: 33.9 kDa
• Gene Summary: C6ORF55 encodes a protein involved in trafficking of the multivesicular body, an endosomal compartment involved in sorting membrane proteins for degradation in lysosomes (Ward et al., 2005 [PubMed 15644320]). [supplied by OMIM, Mar 2008]