PIWIL2 (NM_018068) Human Tagged ORF Clone

CAT#: RC205464

PIWIL2 (Myc-DDK-tagged)-Human piwi-like 2 (Drosophila) (PIWIL2), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY427682) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227303 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PIWIL2
• Synonyms: CT80; HILI; mili; PIWIL1L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205464 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCTTTCCGACCATCGTTCAGGGGCCAGTCTCCTATCCACCCATCCCAGTGCCAGGCTGTACGGA
  TGCCAGGCTGTTGGCCACAAGCTTCTAAACCTTTGGACCCAGCTCTGGGCAGGGGAGCACCTGCAGGCAG
  AGGCCATGTATTTGGAAAGCCAGAGGAACCAAGCACACAGAGGGGGCCAGCACAAAGGGAGTCTGTGGGT
  TTGGTCTCCATGTTCCGAGGCCTGGGCATTGAAACAGTTTCTAAGACCCCTCTGAAACGGGAAATGCTTC
  CATCAGGTAGAGGCATTTTAGGTCGAGGCTTGTCTGCTAATCTGGTACGCAAGGACAGGGAGGAACTCTC
  TCCCACTTTTTGGGATCCAAAAGTGTTGGCGGCTGGGGACAGCAAGATGGCAGAGACCTCCGTTGGTTGG
  AGTAGGACGCTTGGAAGAGGGAGTTCAGATGCGTCTTTATTACCACTGGGAAGAGCAGCAGGTGGTATCA
  GCAGAGAAGTGGACAAGCCTCCCTGTACCTTCAGCACACCGTCCCGGGGTCCCCCGCAGCTGTCATCACC
  ACCAGCTCTGCCCCAGTCTCCCCTGCACTCTCCAGATCGCCCTCTGGTCCTGACTGTGGAACACAAGGAA
  AAAGAGCTTATTGTGAAGCAAGGATCAAAAGGAACACCTCAGTCTTTGGGACTGAACCTCGTCAAAATAC
  AGTGTCATAATGAAGCAGTTTATCAATATCATGTGACTTTCAGCCCCAATGTGGAGTGCAAAAGCATGAG
  GTTCGGCATGTTGAAGGACCATCAAGCTGTCACCGGCAACGTCACTGCGTTTGATGGATCTATTCTCTAT
  CTGCCTGTTAAGCTTCAACAAGTTCTTGAGTTAAAAAGTCAAAGGAAAACAGACAGTGCTGAAATCAGCA
  TTAAGATTCAGATGACAAAGATCCTGGAGCCCTGCTCTGACCTGTGCATTCCCTTCTACAATGTTGTTTT
  CCGTCGGGTAATGAAACTTTTAGATATGAAGCTTGTGGGGAGAAACTTTTATGACCCTACAAGTGCTATG
  GTACTACAGCAACACAGATTGCAGATCTGGCCAGGCTATGCAGCTAGCATCCGAAGGACAGATGGAGGGC
  TCTTCCTGCTAGCTGATGTCTCCCATAAGGTCATTCGGAATGACTGTGTGCTGGATGTCATGCATGCCAT
  TTATCAGCAGAATAAAGAACACTTCCAGGATGAGTGTACTAAGCTTCTGGTTGGCAATATTGTTATCACC
  CGATATAACAATCGTACCTATCGTATTGATGATGTGGATTGGAATAAGACTCCAAAGGATAGCTTCACGA
  TGTCTGATGGGAAAGAGATCACATTCTTGGAATACTACAGCAAAAATTATGGGATCACAGTTAAGGAAGA
  GGACCAGCCATTGCTGATTCACAGGCCCAGTGAGAGACAGGATAATCATGGGATGCTGCTAAAAGGGGAA
  ATCCTGCTGCTGCCTGAGCTTTCTTTTATGACCGGAATCCCAGAGAAGATGAAGAAGGACTTCAGAGCCA
  TGAAGGATTTGGCTCAGCAAATCAATCTGAGCCCCAAGCAACACCATAGTGCTTTGGAATGCTTGCTGCA
  AAGAATTGCAAAGAACGAGGCAGCCACCAATGAACTGATGCGTTGGGGGCTCCGTCTGCAAAAGGATGTA
  CATAAGATTGAAGGACGTGTTCTGCCAATGGAAAGAATTAACTTAAAAAATACTTCGTTTATCACATCTC
  AGGAACTAAACTGGGTTAAGGAAGTAACCAGAGACCCTTCCATCTTGACTATCCCCATGCATTTCTGGGC
  ACTTTTTTACCCAAAGAGAGCAATGGACCAGGCTCGAGAACTGGTCAACATGTTGGAGAAGATAGCCGGC
  CCCATTGGCATGCGTATGAGCCCACCGGCCTGGGTTGAACTAAAGGATGACCGAATAGAGACTTATGTCA
  GAACCATTCAATCCACGTTAGGAGCTGAGGGGAAGATACAGATGGTTGTTTGCATCATCATGGGCCCACG
  TGATGATCTCTATGGGGCCATCAAGAAGCTGTGCTGTGTGCAGTCCCCAGTGCCCTCCCAGGTTGTCAAT
  GTTCGAACCATTGGTCAGCCCACCAGGCTTCGGAGTGTGGCCCAGAAGATTTTACTTCAGATTAACTGTA
  AATTGGGTGGTGAGCTCTGGGGAGTGGATATTCCTCTGAAACAGTTAATGGTGATCGGGATGGATGTTTA
  CCATGACCCCAGTAGAGGCATGCGCTCCGTGGTTGGCTTCGTGGCAAGCATCAATCTCACCCTCACAAAA
  TGGTATTCCCGGGTGGTGTTCCAGATGCCGCATCAGGAGATTGTGGACAGCCTGAAGCTATGCCTCGTGG
  GCTCCTTAAAAAAGTTTTATGAGGTGAACCACTGTCTACCAGAGAAGATTGTGGTGTACCGTGATGGAGT
  GTCTGATGGCCAACTGAAGACAGTTGCCAACTATGAGATTCCTCAACTACAGAAGTGTTTTGAAGCTTTT
  GAGAATTATCAGCCCAAGATGGTGGTGTTTGTAGTTCAGAAGAAAATCAGTACTAATCTATATCTGGCTG
  CTCCTCAGAACTTTGTAACTCCCACTCCTGGAACTGTGGTAGATCATACAATAACAAGCTGTGAGTGGGT
  GGATTTCTATCTTCTTGCCCATCATGTACGGCAGGGCTGTGGCATTCCTACGCATTATGTCTGTGTTCTC
  AACACCGCAAACCTGAGCCCTGATCATATGCAGAGGCTGACTTTCAAACTGTGCCACATGTACTGGAATT
  GGCCTGGCACCATCAGAGTTCCAGCTCCTTGCAAGTATGCCCACAAGCTAGCTTTCCTGTCAGGACACAT
  CTTGCATCATGAACCAGCCATCCAGCTGTGCGAGAACCTGTTCTTCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205464 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDPFRPSFRGQSPIHPSQCQAVRMPGCWPQASKPLDPALGRGAPAGRGHVFGKPEEPSTQRGPAQRESVG
  LVSMFRGLGIETVSKTPLKREMLPSGRGILGRGLSANLVRKDREELSPTFWDPKVLAAGDSKMAETSVGW
  SRTLGRGSSDASLLPLGRAAGGISREVDKPPCTFSTPSRGPPQLSSPPALPQSPLHSPDRPLVLTVEHKE
  KELIVKQGSKGTPQSLGLNLVKIQCHNEAVYQYHVTFSPNVECKSMRFGMLKDHQAVTGNVTAFDGSILY
  LPVKLQQVLELKSQRKTDSAEISIKIQMTKILEPCSDLCIPFYNVVFRRVMKLLDMKLVGRNFYDPTSAM
  VLQQHRLQIWPGYAASIRRTDGGLFLLADVSHKVIRNDCVLDVMHAIYQQNKEHFQDECTKLLVGNIVIT
  RYNNRTYRIDDVDWNKTPKDSFTMSDGKEITFLEYYSKNYGITVKEEDQPLLIHRPSERQDNHGMLLKGE
  ILLLPELSFMTGIPEKMKKDFRAMKDLAQQINLSPKQHHSALECLLQRIAKNEAATNELMRWGLRLQKDV
  HKIEGRVLPMERINLKNTSFITSQELNWVKEVTRDPSILTIPMHFWALFYPKRAMDQARELVNMLEKIAG
  PIGMRMSPPAWVELKDDRIETYVRTIQSTLGAEGKIQMVVCIIMGPRDDLYGAIKKLCCVQSPVPSQVVN
  VRTIGQPTRLRSVAQKILLQINCKLGGELWGVDIPLKQLMVIGMDVYHDPSRGMRSVVGFVASINLTLTK
  WYSRVVFQMPHQEIVDSLKLCLVGSLKKFYEVNHCLPEKIVVYRDGVSDGQLKTVANYEIPQLQKCFEAF
  ENYQPKMVVFVVQKKISTNLYLAAPQNFVTPTPGTVVDHTITSCEWVDFYLLAHHVRQGCGIPTHYVCVL
  NTANLSPDHMQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGHILHHEPAIQLCENLFFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018068
• ORF Size: 2919 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018068.2, NM_018068.3, NP_060538.2
• RefSeq Size: 3636 bp
• RefSeq ORF: 2922 bp
• Locus ID: 55124
• Domains: PAZ, Piwi
• Protein Pathways: Dorso-ventral axis formation
• MW: 109.8 kDa
• Gene Summary: PIWIL2 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]). [supplied by OMIM, Mar 2008]