WDR48 (NM_020839) Human Tagged ORF Clone

CAT#: RC205497

WDR48 (Myc-DDK-tagged)-Human WD repeat domain 48 (WDR48)

Product Images

Western blot validation of overexpression lysate (Cat# LY412261) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205497 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified WDR48 protein (Cat# TP305497). The protein was produced from HEK293T cells transfected with WDR48 cDNA clone (Cat# RC205497) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WDR48
• Synonyms: P80; SPG60; UAF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205497 representing NM_020839
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCATCACCGGCAGAACACAGCAGGGCGGAGGAAAGTGCAGGTTTCCTATGTTATTCGAGATG
  AAGTGGAGAAGTACAACCGAAATGGAGTCAATGCTCTGCAGCTGGATCCAGCACTAAATAGACTTTTCAC
  AGCCGGTCGAGACTCTATCATAAGAATATGGAGTGTCAATCAGCACAAGCAAGATCCATATATAGCATCT
  ATGGAACACCATACTGATTGGGTAAACGACATTGTACTCTGTTGTAATGGGAAAACATTAATATCTGCTT
  CTTCTGACACGACAGTAAAAGTATGGAATGCACACAAGGGATTTTGCATGTCAACATTAAGGACACATAA
  GGATTACGTAAAGGCCTTAGCATATGCCAAGGATAAAGAACTAGTAGCATCAGCTGGGTTGGACAGACAA
  ATATTCCTTTGGGATGTGAATACTCTAACAGCATTGACTGCCTCAAATAACACTGTCACAACTTCTTCTT
  TAAGTGGAAACAAAGATTCCATTTATAGCCTGGCCATGAATCAACTGGGAACAATCATTGTATCAGGGTC
  CACTGAAAAGGTGTTACGGGTATGGGATCCAAGAACATGTGCAAAACTAATGAAGCTTAAAGGGCACACG
  GATAATGTGAAGGCATTGCTATTAAACAGAGATGGCACGCAATGCCTGTCAGGCAGTTCTGATGGGACAA
  TTCGCCTTTGGTCCCTTGGCCAGCAGAGATGTATAGCAACATACCGAGTCCATGATGAAGGTGTTTGGGC
  GCTGCAAGTCAATGATGCCTTCACACATGTGTATTCTGGTGGAAGGGACAGGAAGATTTATTGTACAGAC
  CTAAGAAACCCTGACATTCGGGTGCTAATTTGTGAAGAAAAAGCACCAGTTCTCAAGATGGAGCTTGATA
  GATCAGCTGATCCTCCTCCTGCAATTTGGGTTGCAACAACTAAGTCTACAGTAAATAAATGGACTTTGAA
  AGGAATTCATAATTTTAGAGCCTCTGGAGATTATGACAATGACTGTACAAATCCTATAACACCTCTTTGT
  ACACAACCTGACCAGGTTATTAAAGGGGGTGCTAGTATTATTCAGTGCCACATTCTTAATGATAAGAGAC
  ATATATTAACCAAAGATACCAATAATAATGTGGCATATTGGGATGTATTGAAGGCATGTAAAGTTGAAGA
  TCTGGGCAAAGTGGATTTTGAAGATGAAATTAAGAAAAGATTTAAAATGGTGTATGTGCCAAATTGGTTC
  TCAGTAGACTTAAAAACAGGGATGTTAACTATTACTTTGGATGAAAGTGATTGTTTTGCTGCCTGGGTTT
  CTGCAAAAGATGCTGGTTTCAGCAGCCCTGATGGGTCAGATCCAAAATTGAATTTAGGAGGACTTTTACT
  CCAAGCACTCCTGGAATATTGGCCTAGAACACATGTGAATCCAATGGATGAAGAGGAAAATGAAGTAAAC
  CATGTAAATGGGGAGCAGGAGAACCGAGTGCAGAAGGGAAATGGATATTTTCAAGTGCCCCCACATACAC
  CCGTGATCTTTGGTGAAGCTGGAGGTCGCACACTGTTCAGGCTGCTCTGCCGAGATTCCGGGGGTGAGAC
  TGAGTCTATGCTTCTTAATGAAACAGTGCCACAATGGGTAATTGACATCACTGTGGATAAAAATATGCCC
  AAATTCAACAAAATTCCTTTCTACCTCCAACCTCATGCATCTTCAGGAGCAAAAACCTTAAAAAAAGATA
  GACTCTCTGCTAGTGACATGCTCCAAGTCCGAAAAGTTATGGAACATGTTTATGAAAAAATTATCAACTT
  GGATAATGAGTCTCAAACCACTAGCTCTTCTAATAATGAAAAACCAGGAGAACAGGAAAAAGAAGAAGAT
  ATTGCTGTGTTGGCAGAGGAGAAAATTGAACTTTTGTGCCAAGACCAGGTTTTGGATCCAAATATGGACC
  TTCGAACAGTGAAACACTTCATATGGAAGAGCGGTGGAGACCTCACCCTCCATTACCGTCAGAAGTCCAC
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205497 representing NM_020839
  Red=Cloning site Green=Tags(s)
  
  MAAHHRQNTAGRRKVQVSYVIRDEVEKYNRNGVNALQLDPALNRLFTAGRDSIIRIWSVNQHKQDPYIAS
  MEHHTDWVNDIVLCCNGKTLISASSDTTVKVWNAHKGFCMSTLRTHKDYVKALAYAKDKELVASAGLDRQ
  IFLWDVNTLTALTASNNTVTTSSLSGNKDSIYSLAMNQLGTIIVSGSTEKVLRVWDPRTCAKLMKLKGHT
  DNVKALLLNRDGTQCLSGSSDGTIRLWSLGQQRCIATYRVHDEGVWALQVNDAFTHVYSGGRDRKIYCTD
  LRNPDIRVLICEEKAPVLKMELDRSADPPPAIWVATTKSTVNKWTLKGIHNFRASGDYDNDCTNPITPLC
  TQPDQVIKGGASIIQCHILNDKRHILTKDTNNNVAYWDVLKACKVEDLGKVDFEDEIKKRFKMVYVPNWF
  SVDLKTGMLTITLDESDCFAAWVSAKDAGFSSPDGSDPKLNLGGLLLQALLEYWPRTHVNPMDEEENEVN
  HVNGEQENRVQKGNGYFQVPPHTPVIFGEAGGRTLFRLLCRDSGGETESMLLNETVPQWVIDITVDKNMP
  KFNKIPFYLQPHASSGAKTLKKDRLSASDMLQVRKVMEHVYEKIINLDNESQTTSSSNNEKPGEQEKEED
  IAVLAEEKIELLCQDQVLDPNMDLRTVKHFIWKSGGDLTLHYRQKST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020839
• ORF Size: 2031 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020839.1, NM_020839.2, NM_020839.3, NP_065890.1
• RefSeq Size: 3705
• RefSeq ORF: 2034
• Locus ID: 57599
• Domains: WD40
• MW: 76 kDa
• Gene Summary: The protein encoded by this gene has been shown to interact with ubiquitin specific peptidase 1 (USP1), activating the deubiquitinating activity of USP1 and allowing it to remove the ubiquitin moiety from monoubiquitinated FANCD2. FANCD2 is ubiquitinated in response to DNA damage. [provided by RefSeq, Sep 2016]