HCG3 (DNAJB3) (NM_001001394) Human Tagged ORF Clone

CAT#: RC205526

DNAJB3 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily B, member 3 (DNAJB3)

Product Images

Western blot validation of overexpression lysate (Cat# LY424336) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205526 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DNAJB3 protein (Cat# TP305526). The protein was produced from HEK293T cells transfected with DNAJB3 cDNA clone (Cat# RC205526) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DNAJB3
• Synonyms: HCG3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205526 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGACTACTACGAGGTGCTGGACGTGCCCCGGCAGGCCTCATCCGAGGCCATCAAGAAGGCGTACC
  GCAAGCTGGCGCTCAAGTGGCACCCCGACAAAAACCCTGAGAACAAGGAGGAAGCGGAGAGGAGATTCAA
  GCAGGTGGCCGAGGCCTACGAGGTGTTGTCGGACGCCAAGAAACGCGATATCTATGACCGCTATGGCGAG
  GCGGGCGCGGAGGGCGGCTGCACAGGCGGCAGGCCCTTCGAGGACCCCTTCGAGTACGTCTTCAGCTTCC
  GCGACCCAGCCGACGTCTTCAGGGAGTTCTTCGGCGGCCAGGACCCATTCTCCTTTGACCTCTTGGGAAA
  CCCGCTGGAGAATATTTTGGGGGGGTCAGAGGAACTGCTGGGGAAGCAGAAGCAGAGCGTCTGCACCCCT
  TTTCTCTGCCTTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205526 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVDYYEVLDVPRQASSEAIKKAYRKLALKWHPDKNPENKEEAERRFKQVAEAYEVLSDAKKRDIYDRYGE
  AGAEGGCTGGRPFEDPFEYVFSFRDPADVFREFFGGQDPFSFDLLGNPLENILGGSEELLGKQKQSVCTP
  FLCLQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001394
• ORF Size: 435 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001394.1, NM_001001394.2, NM_001001394.3, NP_001001394.1
• RefSeq Size: 1266 bp
• RefSeq ORF: 438 bp
• Locus ID: 414061
• MW: 16.6 kDa