Choline dehydrogenase (CHDH) (NM_018397) Human Tagged ORF Clone

CAT#: RC205552

CHDH (Myc-DDK-tagged)-Human choline dehydrogenase (CHDH), nuclear gene encoding mitochondrial protein


  "NM_018397" in other vectors (4)

Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CHDH
Synonyms choline dehydrogenase
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205552 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGTGTCTCCTACGAGGCCTGGGCCGGCCTGGAGCCCTGGCACGGGGAGCCCTGGGGCAGCAGCAAT
CCCTGGGTGCCCGGGCCCTGGCCAGCGCAGGCTCTGAGAGCCGGGACGAGTACAGCTATGTGGTGGTGGG
CGCGGGCTCGGCGGGCTGCGTGCTGGCTGGGAGGCTCACGGAGGACCCCGCCGAGCGCGTGCTGCTGCTG
GAGGCCGGGCCCAAGGACGTGCGCGCGGGGAGCAAGCGGCTCTCGTGGAAGATCCACATGCCCGCGGCCC
TGGTGGCCAACCTGTGCGACGACAGGTACAACTGGTGCTACCACACAGAGGTGCAGCGGGGCCTGGACGG
CCGCGTGCTGTACTGGCCACGCGGCCGCGTCTGGGGTGGCTCCTCATCCCTCAATGCCATGGTCTACGTC
CGTGGGCACGCCGAGGACTACGAGCGCTGGCAGCGCCAGGGCGCCCGCGGCTGGGACTACGCGCACTGCC
TGCCCTACTTCCGCAAGGCGCAGGGCCACGAGCTGGGCGCCAGCCGGTACCGGGGCGCCGATGGCCCGCT
GCGGGTGTCCCGGGGCAAGACCAACCACCCGCTGCACTGCGCATTCCTGGAGGCCACGCAGCAGGCCGGC
TACCCGCTCACCGAGGACATGAATGGCTTCCAGCAGGAGGGCTTCGGCTGGATGGACATGACCATCCATG
AAGGCAAACGGTGGAGCGCAGCCTGTGCCTACCTGCACCCAGCACTGAGCCGCACCAACCTCAAGGCCGA
GGCCGAGACGCTTGTGAGCAGGGTGCTATTTGAGGGCACCCGTGCAGTGGGCGTGGAGTATGTCAAGAAT
GGCCAGAGCCACAGGGCTTATGCCAGCAAGGAGGTGATTCTGAGTGGAGGTGCCATCAACTCTCCACAGC
TGCTCATGCTCTCTGGCATCGGGAATGCTGATGACCTCAAGAAACTGGGCATCCCTGTGGTGTGCCACCT
ACCTGGGGTTGGCCAGAACCTGCAAGACCACCTGGAGATCTACATTCAGCAGGCATGCACCCGCCCTATC
ACCCTCCATTCAGCACAGAAGCCCCTGCGGAAGGTCTGCATTGGTCTGGAGTGGCTCTGGAAATTCACAG
GGGAGGGAGCCACTGCCCATCTGGAAACAGGTGGGTTCATCCGCAGCCAGCCTGGGGTCCCCCACCCGGA
CATCCAGTTCCATTTCCTGCCATCCCAAGTGATTGACCACGGGCGGGTCCCCACCCAGCAGGAGGCTTAC
CAGGTACATGTGGGGCCCATGCGGGGCACGAGTGTGGGCTGGCTCAAACTGAGAAGTGCCAATCCCCAAG
ACCACCCTGTGATCCAGCCCAACTACTTGTCAACAGAAACTGATATTGAGGATTTCCGTCTGTGTGTGAA
GCTCACCAGAGAAATTTTTGCACAGGAAGCCCTGGCTCCGTTCCGAGGGAAAGAGCTCCAGCCAGGAAGC
CACATTCAGTCAGATAAAGAGATAGATGCCTTTGTGCGGGCAAAAGCCGACAGCGCCTACCACCCCTCGT
GCACCTGTAAGATGGGCCAGCCCTCCGATCCCACTGCCGTGGTGGATCCGCAGACAAGGGTCCTCGGGGT
GGAAAACCTCAGGGTCGTCGATGCCTCCATCATGCCTAGCATGGTCAGCGGCAACCTGAACGCCCCCACA
ATCATGATCGCAGAGAAGGCAGCTGACATTATCAAGGGGCAGCCTGCACTCTGGGACAAAGATGTCCCTG
TCTACAAGCCCAGGACGCTGGCCACCCAGCGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205552 protein sequence
Red=Cloning site Green=Tags(s)

MWCLLRGLGRPGALARGALGQQQSLGARALASAGSESRDEYSYVVVGAGSAGCVLAGRLTEDPAERVLLL
EAGPKDVRAGSKRLSWKIHMPAALVANLCDDRYNWCYHTEVQRGLDGRVLYWPRGRVWGGSSSLNAMVYV
RGHAEDYERWQRQGARGWDYAHCLPYFRKAQGHELGASRYRGADGPLRVSRGKTNHPLHCAFLEATQQAG
YPLTEDMNGFQQEGFGWMDMTIHEGKRWSAACAYLHPALSRTNLKAEAETLVSRVLFEGTRAVGVEYVKN
GQSHRAYASKEVILSGGAINSPQLLMLSGIGNADDLKKLGIPVVCHLPGVGQNLQDHLEIYIQQACTRPI
TLHSAQKPLRKVCIGLEWLWKFTGEGATAHLETGGFIRSQPGVPHPDIQFHFLPSQVIDHGRVPTQQEAY
QVHVGPMRGTSVGWLKLRSANPQDHPVIQPNYLSTETDIEDFRLCVKLTREIFAQEALAPFRGKELQPGS
HIQSDKEIDAFVRAKADSAYHPSCTCKMGQPSDPTAVVDPQTRVLGVENLRVVDASIMPSMVSGNLNAPT
IMIAEKAADIIKGQPALWDKDVPVYKPRTLATQR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_018397
ORF Size 1782 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Reference Data
RefSeq NM_018397.1, NM_018397.2, NM_018397.3, NM_018397.4, NP_060867.1, NP_060867.2
RefSeq Size 3711
RefSeq ORF 1785
Locus ID 55349
Protein Pathways Glycine, serine and threonine metabolism
MW 65.4 kDa
Gene Summary The protein encoded by this gene is a choline dehydrogenase that localizes to the mitochondrion. Variations in this gene can affect susceptibility to choline deficiency. A few transcript variants have been found for this gene, but the full-length nature of only one has been characterized to date. [provided by RefSeq, Dec 2010]

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