RBKS (NM_022128) Human Tagged ORF Clone

CAT#: RC205636

RBKS (Myc-DDK-tagged)-Human ribokinase (RBKS)

Product Images

Western blot validation of overexpression lysate (Cat# LY411756) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205636 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RBKS protein (Cat# TP305636). The protein was produced from HEK293T cells transfected with RBKS cDNA clone (Cat# RC205636) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RBKS
• Synonyms: RBSK; RK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205636 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGTCTGGGGAACCCCAGAGGCAGTGGCAAGAGGAGGTGGCGGCGGTGGTAGTGGTGGGCTCCT
  GCATGACCGACCTGGTCAGTCTTACTTCTCGTTTGCCAAAAACTGGAGAAACCATCCATGGACATAAGTT
  TTTTATTGGCTTTGGAGGGAAAGGTGCCAACCAGTGTGTCCAAGCTGCTCGGCTTGGAGCAATGACGTCC
  ATGGTGTGTAAGGTTGGCAAAGATTCTTTTGGCAATGATTATATAGAAAACTTAAAACAGAATGATATTT
  CTACAGAATTTACATATCAGACTAAAGATGCTGCTACAGGAACTGCTTCTATAATTGTCAATAATGAAGG
  CCAGAATATCATTGTCATAGTGGCTGGAGCAAATTTACTTTTGAATACGGAGGATCTGAGGGCAGCAGCC
  AATGTCATTAGCAGAGCCAAAGTCATGGTCTGCCAGCTCGAAATAACTCCAGCAACTTCTTTGGAAGCCC
  TAACAATGGCCCGCAGGAGTGGAGTGAAAACCTTGTTCAATCCAGCCCCTGCCATTGCTGACCTGGATCC
  CCAGTTCTACACCCTCTCAGATGTGTTCTGCTGCAATGAAAGTGAGGCTGAGATTTTAACTGGCCTCACG
  GTGGGCAGCGCTGCAGATGCTGGGGAGGCTGCATTAGTGCTCTTGAAAAGGGGCTGCCAGGTGGTAATCA
  TTACCTTAGGGGCTGAAGGATGTGTGGTGCTGTCACAGACAGAACCTGAGCCAAAGCACATTCCCACAGA
  GAAAGTCAAGGCTGTGGATACCACGGGTGCTGGTGACAGCTTTGTGGGAGCTCTGGCCTTCTACCTGGCT
  TACTATCCAAATCTGTCCTTGGAAGACATGCTCAACAGATCCAATTTCATTGCAGCAGTCAGTGTCCAGG
  CTGCAGGAACACAGTCATCTTACCCTTACAAAAAAGACCTTCCGCTTACTCTGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205636 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAASGEPQRQWQEEVAAVVVVGSCMTDLVSLTSRLPKTGETIHGHKFFIGFGGKGANQCVQAARLGAMTS
  MVCKVGKDSFGNDYIENLKQNDISTEFTYQTKDAATGTASIIVNNEGQNIIVIVAGANLLLNTEDLRAAA
  NVISRAKVMVCQLEITPATSLEALTMARRSGVKTLFNPAPAIADLDPQFYTLSDVFCCNESEAEILTGLT
  VGSAADAGEAALVLLKRGCQVVIITLGAEGCVVLSQTEPEPKHIPTEKVKAVDTTGAGDSFVGALAFYLA
  YYPNLSLEDMLNRSNFIAAVSVQAAGTQSSYPYKKDLPLTLF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022128
• ORF Size: 966 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022128.1, NM_022128.2, NP_071411.1
• RefSeq Size: 1276 bp
• RefSeq ORF: 969 bp
• Locus ID: 64080
• Protein Families: Druggable Genome
• Protein Pathways: Pentose phosphate pathway
• MW: 34.1 kDa
• Gene Summary: This gene encodes a member of the carbohydrate kinase PfkB family. The encoded protein phosphorylates ribose to form ribose-5-phosphate in the presence of ATP and magnesium as a first step in ribose metabolism. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]