GAL3ST1 (NM_004861) Human Tagged ORF Clone

CAT#: RC205706

GAL3ST1 (Myc-DDK-tagged)-Human galactose-3-O-sulfotransferase 1 (GAL3ST1)

Product Images

Western blot validation of overexpression lysate (Cat# LY417697) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205706 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GAL3ST1
• Synonyms: CST
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205706 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCACCGCAGAAGAAGCCCTGGGAGTCCATGGCTAAGGGGCTGGTGCTGGGCGCGCTCTTCACTA
  GTTTCCTGCTGCTGATGTACTCCTATGCCGTGCCCCCGCTGCATGCCGGCCTGGCCTCCACGACCCCGGA
  GGCCGCAGCGTCCTGCTCTCCACCTGCACTCGAGCCAGAGGCAGTGATCCGGGCCAACGGCTCGGCGGGG
  GAGTGCCAGCCGCGGCGCAACATCGTGTTCTTGAAGACGCACAAGACGGCCAGCAGCACCCTGCTCAACA
  TCCTGTTCCGCTTCGGCCAGAAGCACCGGCTCAAGTTCGCCTTCCCTAACGGCCGCAATGACTTCGACTA
  CCCGACCTTCTTCGCCCGCAGCCTGGTGCAGGACTATCGGCCCGGGGCCTGCTTCAACATCATCTGCAAC
  CACATGCGCTTCCACTACGACGAGGTGCGCGGCCTGGTGCCGACCAACGCCATCTTCATCACGGTGCTCC
  GCGACCCCGCCCGCTTGTTCGAGTCCTCCTTCCACTACTTCGGGCCGGTGGTGCCCCTCACGTGGAAGCT
  CTCGGCCGGCGACAAGCTGACCGAGTTCCTGCAAGACCCGGATCGCTACTACGACCCCAACGGCTTCAAT
  GCCCACTACCTCCGAAACCTGCTCTTCTTCGACCTGGGCTATGACAACAGCCTGGACCCCAGCAGCCCGC
  AGGTGCAGGAGCACATCCTGGAGGTGGAGCGTCGCTTCCACCTGGTGCTCCTTCAAGAGTACTTCGACGA
  GTCGCTGGTGCTGCTGAAGGACCTGCTGTGCTGGGAGCTGGAGGACGTGCTCTACTTCAAGCTCAACGCC
  CGCCGCGACTCGCCCGTGCCGCGGCTCTCGGGGGAGCTGTATGGGCGCGCCACCGCCTGGAACATGCTGG
  ACTCCCACCTCTACCGCCACTTCAACGCCAGCTTCTGGCGCAAGGTGGAGGCCTTCGGGCGGGAGCGCAT
  GGCCCGCGAGGTGGCCGCCCTGCGCCATGCCAACGAGCGCATGCGGACCATCTGCATCGACGGGGGCCAC
  GCCGTGGACGCCGCCGCCATCCAGGACGAGGCCATGCAGCCCTGGCAGCCGCTGGGCACCAAGTCCATCC
  TGGGCTACAACCTCAAGAAGAGCATCGGGCAGCGGCACGCGCAGCTCTGCCGGCGCATGCTCACGCCCGA
  GATCCAGTACCTGATGGACCTCGGCGCCAACCTGTGGGTCACCAAGCTCTGGAAGTTCATTCGCGATTTC
  CTGCGGTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205706 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLPPQKKPWESMAKGLVLGALFTSFLLLMYSYAVPPLHAGLASTTPEAAASCSPPALEPEAVIRANGSAG
  ECQPRRNIVFLKTHKTASSTLLNILFRFGQKHRLKFAFPNGRNDFDYPTFFARSLVQDYRPGACFNIICN
  HMRFHYDEVRGLVPTNAIFITVLRDPARLFESSFHYFGPVVPLTWKLSAGDKLTEFLQDPDRYYDPNGFN
  AHYLRNLLFFDLGYDNSLDPSSPQVQEHILEVERRFHLVLLQEYFDESLVLLKDLLCWELEDVLYFKLNA
  RRDSPVPRLSGELYGRATAWNMLDSHLYRHFNASFWRKVEAFGRERMAREVAALRHANERMRTICIDGGH
  AVDAAAIQDEAMQPWQPLGTKSILGYNLKKSIGQRHAQLCRRMLTPEIQYLMDLGANLWVTKLWKFIRDF
  LRW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004861
• ORF Size: 1269 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004861.1, NM_004861.2, NP_004852.1
• RefSeq Size: 1791 bp
• RefSeq ORF: 1272 bp
• Locus ID: 9514
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, Sphingolipid metabolism
• MW: 48.8 kDa
• Gene Summary: Sulfonation, an important step in the metabolism of many drugs, xenobiotics, hormones, and neurotransmitters, is catalyzed by sulfotransferases. This gene encodes galactosylceramide sulfotransferase, which catalyzes the sulfation of membrane glycolipids including the final step in the synthesis of sulfatide, a major lipid component of the myelin sheath. This gene exhibits elevated expression in ovarian epithelial carcinoma and the encoded enzyme exhibits elevated activity in renal cell carcinoma. Mutations in this gene may be associated with reduced insulin resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]