KDEL Receptor (KDELR1) (NM_006801) Human Tagged ORF Clone

CAT#: RC205880

KDELR1 (Myc-DDK-tagged)-Human KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1 (KDELR1)

Product Images

Western blot validation of overexpression lysate (Cat# LY416412) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205880 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KDELR1 protein (Cat# TP305880). The protein was produced from HEK293T cells transfected with KDELR1 cDNA clone (Cat# RC205880) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KDELR1
• Synonyms: ERD2; ERD2.1; HDEL; PM23
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205880 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCTCTTCCGATTCCTGGGAGACCTCTCCCACCTCCTCGCCATCATCTTGCTACTGCTCAAAATCT
  GGAAGTCCCGCTCGTGCGCCGGAATTTCAGGGAAGAGCCAGGTCCTGTTTGCTGTGGTGTTCACTGCCCG
  ATATCTGGACCTCTTCACCAACTACATCTCACTCTACAACACGTGTATGAAGGTGGTCTACATAGCCTGC
  TCCTTCACCACGGTCTGGTTGATTTATAGCAAGTTCAAAGCTACTTACGATGGGAACCATGACACGTTCA
  GAGTGGAGTTCCTGGTCGTTCCCACAGCCATTCTGGCGTTCCTGGTCAATCATGACTTCACCCCTCTGGA
  GATCCTCTGGACCTTCTCCATCTACCTGGAGTCAGTGGCCATCTTGCCGCAGCTGTTCATGGTGAGCAAG
  ACCGGCGAGGCGGAGACCATCACCAGCCACTACTTGTTTGCGCTAGGCGTTTACCGCACGCTCTATCTCT
  TCAACTGGATCTGGCGCTACCATTTCGAGGGCTTCTTCGACCTCATCGCCATTGTGGCAGGCCTGGTCCA
  GACAGTCCTCTACTGCGATTTCTTCTACCTCTATATCACCAAAGTCCTAAAGGGGAAGAAGTTGAGTTTG
  CCGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205880 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNLFRFLGDLSHLLAIILLLLKIWKSRSCAGISGKSQVLFAVVFTARYLDLFTNYISLYNTCMKVVYIAC
  SFTTVWLIYSKFKATYDGNHDTFRVEFLVVPTAILAFLVNHDFTPLEILWTFSIYLESVAILPQLFMVSK
  TGEAETITSHYLFALGVYRTLYLFNWIWRYHFEGFFDLIAIVAGLVQTVLYCDFFYLYITKVLKGKKLSL
  PA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006801
• ORF Size: 636 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006801.1, NM_006801.2, NP_006792.1
• RefSeq Size: 1575 bp
• RefSeq ORF: 639 bp
• Locus ID: 10945
• Domains: ER_lumen_recept
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Vibrio cholerae infection
• MW: 24.5 kDa
• Gene Summary: Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product. [provided by RefSeq, Jul 2008]