DHX15 (NM_001358) Human Tagged ORF Clone
CAT#: RC205914
DHX15 (Myc-DDK-tagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 15 (DHX15)
"NM_001358" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | DHX15 |
Synonyms | DBP1; DDX15; HRH2; PRP43; PRPF43; PrPp43p |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC205914 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAAGCGGCACCGGTTGGACCTAGGGGAGGATTACCCCTCTGGCAAGAAGCGTGCGGGGACCGATG GGAAGGATCGAGATCGAGACCGGGATCGTGAAGATCGGTCTAAAGATCGAGACCGAGAACGTGATAGAGG AGATAGAGAGCGAGAGAGGGAGAAAGAAAAGGAGAAGGAGTTGCGAGCTTCAACAAATGCTATGCTTATC AGTGCTGGATTACCACCTTTGAAAGCTTCCCATTCAGCTCACTCAACCCACTCAGCACATTCAACGCATT CAACACATTCTGCTCATTCAACGCATGCCGGACATGCAGGTCACACGTCACTTCCACAGTGCATTAATCC GTTCACCAACTTACCCCATACTCCTCGATACTATGATATTCTAAAGAAACGTCTTCAGCTCCCTGTTTGG GAATACAAGGATAGGTTTACAGATATTCTGGTTAGACATCAGTCCTTTGTACTGGTTGGTGAGACTGGGT CTGGTAAAACAACACAGATTCCACAGTGGTGTGTGGAGTACATGCGATCATTACCAGGACCCAAGAGAGG AGTTGCCTGTACCCAACCCAGGAGAGTGGCTGCAATGAGTGTGGCTCAGAGAGTTGCTGATGAGATGGAT GTGATGTTGGGCCAGGAAGTTGGTTACTCCATTCGATTTGAAGACTGCAGTAGTGCAAAAACCATTCTTA AGTATATGACTGATGGGATGTTACTTCGTGAAGCTATGAATGATCCCCTCCTGGAGCGTTATGGTGTAAT AATTCTTGATGAGGCTCATGAGAGGACACTGGCTACAGATATTCTAATGGGTGTTCTGAAGGAAGTTGTA AGACAGAGATCAGATTTAAAGGTTATAGTTATGAGCGCTACTCTAGATGCAGGAAAATTCCAGATTTACT TTGATAACTGTCCTCTCCTAACTATTCCTGGGCGTACACATCCTGTTGAGATCTTCTATACTCCAGAACC AGAGAGAGATTATCTTGAAGCAGCAATTCGAACAGTTATCCAGATTCATATGTGTGAAGAGGAAGAGGGA GATCTTCTTCTTTTCTTAACTGGTCAAGAGGAAATTGATGAAGCCTGTAAGAGAATAAAGCGTGAAGTTG ATGATTTGGGCCCTGAAGTTGGTGACATTAAAATCATTCCATTGTATTCTACACTTCCACCTCAGCAGCA GCAACGCATTTTTGAGCCTCCACCTCCCAAAAAACAGAATGGAGCAATTGGAAGAAAGGTAGTTGTGTCA ACTAACATAGCAGAGACGTCTTTGACAATAGATGGTGTGGTGTTTGTGATTGATCCTGGATTTGCGAAAC AGAAGGTCTACAATCCTCGAATCAGAGTTGAGTCCCTTTTGGTGACAGCTATTAGTAAAGCTTCAGCTCA GCAAAGGGCTGGTCGAGCTGGACGTACCAGACCTGGAAAATGCTTCAGACTTTACACAGAGAAAGCTTAT AAAACAGAAATGCAGGATAACACCTATCCTGAGATTTTGCGTTCTAATTTAGGATCAGTTGTGTTACAAT TGAAGAAACTTGGTATTGATGACTTGGTACATTTTGATTTTATGGATCCACCAGCTCCTGAAACTCTGAT GAGAGCCCTGGAACTTTTGAATTACCTGGCTGCTTTAAATGATGATGGAGATCTGACTGAATTGGGATCC ATGATGGCAGAGTTTCCTCTAGATCCACAGCTCGCAAAAATGGTTATTGCAAGTTGTGACTACAACTGTT CTAATGAGGTCCTATCTATTACTGCTATGTTGTCAGTCCCACAGTGTTTTGTTCGCCCCACGGAGGCCAA GAAAGCCGCAGATGAGGCCAAGATGAGATTTGCCCACATAGATGGAGATCATCTGACACTGCTGAACGTC TACCATGCTTTTAAACAAAATCATGAATCGGTTCAGTGGTGTTATGACAACTTCATTAACTACAGGTCCC TGATGTCCGCAGACAATGTACGCCAGCAGCTATCTCGAATTATGGACAGATTTAATTTGCCTCGTCGAAG TACTGACTTTACAAGCAGGGACTATTATATTAATATAAGAAAAGCTTTGGTTACTGGGTATTTTATGCAG GTGGCACATTTAGAACGAACAGGGCATTACTTAACTGTGAAAGATAACCAGGTGGTTCAGTTGCATCCCT CTACTGTTCTTGACCACAAACCTGAATGGGTGCTTTATAATGAGTTTGTTCTAACAACAAAGAATTACAT CCGGACATGTACAGATATCAAGCCAGAATGGTTGGTGAAAATTGCCCCTCAATATTATGACATGAGCAAT TTCCCACAGTGTGAAGCAAAGAGACAGTTGGACCGCATCATTGCCAAACTTCAATCCAAGGAATATTCAC AGTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205914 protein sequence
Red=Cloning site Green=Tags(s) MSKRHRLDLGEDYPSGKKRAGTDGKDRDRDRDREDRSKDRDRERDRGDREREREKEKEKELRASTNAMLI SAGLPPLKASHSAHSTHSAHSTHSTHSAHSTHAGHAGHTSLPQCINPFTNLPHTPRYYDILKKRLQLPVW EYKDRFTDILVRHQSFVLVGETGSGKTTQIPQWCVEYMRSLPGPKRGVACTQPRRVAAMSVAQRVADEMD VMLGQEVGYSIRFEDCSSAKTILKYMTDGMLLREAMNDPLLERYGVIILDEAHERTLATDILMGVLKEVV RQRSDLKVIVMSATLDAGKFQIYFDNCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHMCEEEEG DLLLFLTGQEEIDEACKRIKREVDDLGPEVGDIKIIPLYSTLPPQQQQRIFEPPPPKKQNGAIGRKVVVS TNIAETSLTIDGVVFVIDPGFAKQKVYNPRIRVESLLVTAISKASAQQRAGRAGRTRPGKCFRLYTEKAY KTEMQDNTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALNDDGDLTELGS MMAEFPLDPQLAKMVIASCDYNCSNEVLSITAMLSVPQCFVRPTEAKKAADEAKMRFAHIDGDHLTLLNV YHAFKQNHESVQWCYDNFINYRSLMSADNVRQQLSRIMDRFNLPRRSTDFTSRDYYINIRKALVTGYFMQ VAHLERTGHYLTVKDNQVVQLHPSTVLDHKPEWVLYNEFVLTTKNYIRTCTDIKPEWLVKIAPQYYDMSN FPQCEAKRQLDRIIAKLQSKEYSQY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001358 |
ORF Size | 2385 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001358.2, NP_001349.2 |
RefSeq Size | 3019 bp |
RefSeq ORF | 2388 bp |
Locus ID | 1665 |
Domains | DEAD, helicase_C, HA2 |
Protein Pathways | Spliceosome |
MW | 90.9 kDa |
Gene Summary | The protein encoded by this gene is a putative ATP-dependent RNA helicase implicated in pre-mRNA splicing. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC119280 | DHX15 (untagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 15 (DHX15) |
USD 760.00 |
|
RG205914 | DHX15 (GFP-tagged) - Human DEAH (Asp-Glu-Ala-His) box polypeptide 15 (DHX15) |
USD 460.00 |
|
RC205914L1 | Lenti ORF clone of Human DEAH (Asp-Glu-Ala-His) box polypeptide 15 (DHX15), Myc-DDK-tagged |
USD 620.00 |
|
RC205914L2 | Lenti ORF clone of Human DEAH (Asp-Glu-Ala-His) box polypeptide 15 (DHX15), mGFP tagged |
USD 620.00 |
|
RC205914L3 | Lenti ORF clone of Human DEAH (Asp-Glu-Ala-His) box polypeptide 15 (DHX15), Myc-DDK-tagged |
USD 620.00 |
|
RC205914L4 | Lenti ORF clone of Human DEAH (Asp-Glu-Ala-His) box polypeptide 15 (DHX15), mGFP tagged |
USD 768.00 |
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