DHX15 (NM_001358) Human Tagged ORF Clone

CAT#: RC205914

DHX15 (Myc-DDK-tagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 15 (DHX15)

Product Images

Western blot validation of overexpression lysate (Cat# LY419972) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205914 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified DHX15 protein (Cat# TP305914). The protein was produced from HEK293T cells transfected with DHX15 cDNA clone (Cat# RC205914) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DHX15
• Synonyms: DBP1; DDX15; HRH2; PRP43; PRPF43; PrPp43p
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205914 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAAGCGGCACCGGTTGGACCTAGGGGAGGATTACCCCTCTGGCAAGAAGCGTGCGGGGACCGATG
  GGAAGGATCGAGATCGAGACCGGGATCGTGAAGATCGGTCTAAAGATCGAGACCGAGAACGTGATAGAGG
  AGATAGAGAGCGAGAGAGGGAGAAAGAAAAGGAGAAGGAGTTGCGAGCTTCAACAAATGCTATGCTTATC
  AGTGCTGGATTACCACCTTTGAAAGCTTCCCATTCAGCTCACTCAACCCACTCAGCACATTCAACGCATT
  CAACACATTCTGCTCATTCAACGCATGCCGGACATGCAGGTCACACGTCACTTCCACAGTGCATTAATCC
  GTTCACCAACTTACCCCATACTCCTCGATACTATGATATTCTAAAGAAACGTCTTCAGCTCCCTGTTTGG
  GAATACAAGGATAGGTTTACAGATATTCTGGTTAGACATCAGTCCTTTGTACTGGTTGGTGAGACTGGGT
  CTGGTAAAACAACACAGATTCCACAGTGGTGTGTGGAGTACATGCGATCATTACCAGGACCCAAGAGAGG
  AGTTGCCTGTACCCAACCCAGGAGAGTGGCTGCAATGAGTGTGGCTCAGAGAGTTGCTGATGAGATGGAT
  GTGATGTTGGGCCAGGAAGTTGGTTACTCCATTCGATTTGAAGACTGCAGTAGTGCAAAAACCATTCTTA
  AGTATATGACTGATGGGATGTTACTTCGTGAAGCTATGAATGATCCCCTCCTGGAGCGTTATGGTGTAAT
  AATTCTTGATGAGGCTCATGAGAGGACACTGGCTACAGATATTCTAATGGGTGTTCTGAAGGAAGTTGTA
  AGACAGAGATCAGATTTAAAGGTTATAGTTATGAGCGCTACTCTAGATGCAGGAAAATTCCAGATTTACT
  TTGATAACTGTCCTCTCCTAACTATTCCTGGGCGTACACATCCTGTTGAGATCTTCTATACTCCAGAACC
  AGAGAGAGATTATCTTGAAGCAGCAATTCGAACAGTTATCCAGATTCATATGTGTGAAGAGGAAGAGGGA
  GATCTTCTTCTTTTCTTAACTGGTCAAGAGGAAATTGATGAAGCCTGTAAGAGAATAAAGCGTGAAGTTG
  ATGATTTGGGCCCTGAAGTTGGTGACATTAAAATCATTCCATTGTATTCTACACTTCCACCTCAGCAGCA
  GCAACGCATTTTTGAGCCTCCACCTCCCAAAAAACAGAATGGAGCAATTGGAAGAAAGGTAGTTGTGTCA
  ACTAACATAGCAGAGACGTCTTTGACAATAGATGGTGTGGTGTTTGTGATTGATCCTGGATTTGCGAAAC
  AGAAGGTCTACAATCCTCGAATCAGAGTTGAGTCCCTTTTGGTGACAGCTATTAGTAAAGCTTCAGCTCA
  GCAAAGGGCTGGTCGAGCTGGACGTACCAGACCTGGAAAATGCTTCAGACTTTACACAGAGAAAGCTTAT
  AAAACAGAAATGCAGGATAACACCTATCCTGAGATTTTGCGTTCTAATTTAGGATCAGTTGTGTTACAAT
  TGAAGAAACTTGGTATTGATGACTTGGTACATTTTGATTTTATGGATCCACCAGCTCCTGAAACTCTGAT
  GAGAGCCCTGGAACTTTTGAATTACCTGGCTGCTTTAAATGATGATGGAGATCTGACTGAATTGGGATCC
  ATGATGGCAGAGTTTCCTCTAGATCCACAGCTCGCAAAAATGGTTATTGCAAGTTGTGACTACAACTGTT
  CTAATGAGGTCCTATCTATTACTGCTATGTTGTCAGTCCCACAGTGTTTTGTTCGCCCCACGGAGGCCAA
  GAAAGCCGCAGATGAGGCCAAGATGAGATTTGCCCACATAGATGGAGATCATCTGACACTGCTGAACGTC
  TACCATGCTTTTAAACAAAATCATGAATCGGTTCAGTGGTGTTATGACAACTTCATTAACTACAGGTCCC
  TGATGTCCGCAGACAATGTACGCCAGCAGCTATCTCGAATTATGGACAGATTTAATTTGCCTCGTCGAAG
  TACTGACTTTACAAGCAGGGACTATTATATTAATATAAGAAAAGCTTTGGTTACTGGGTATTTTATGCAG
  GTGGCACATTTAGAACGAACAGGGCATTACTTAACTGTGAAAGATAACCAGGTGGTTCAGTTGCATCCCT
  CTACTGTTCTTGACCACAAACCTGAATGGGTGCTTTATAATGAGTTTGTTCTAACAACAAAGAATTACAT
  CCGGACATGTACAGATATCAAGCCAGAATGGTTGGTGAAAATTGCCCCTCAATATTATGACATGAGCAAT
  TTCCCACAGTGTGAAGCAAAGAGACAGTTGGACCGCATCATTGCCAAACTTCAATCCAAGGAATATTCAC
  AGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205914 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSKRHRLDLGEDYPSGKKRAGTDGKDRDRDRDREDRSKDRDRERDRGDREREREKEKEKELRASTNAMLI
  SAGLPPLKASHSAHSTHSAHSTHSTHSAHSTHAGHAGHTSLPQCINPFTNLPHTPRYYDILKKRLQLPVW
  EYKDRFTDILVRHQSFVLVGETGSGKTTQIPQWCVEYMRSLPGPKRGVACTQPRRVAAMSVAQRVADEMD
  VMLGQEVGYSIRFEDCSSAKTILKYMTDGMLLREAMNDPLLERYGVIILDEAHERTLATDILMGVLKEVV
  RQRSDLKVIVMSATLDAGKFQIYFDNCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHMCEEEEG
  DLLLFLTGQEEIDEACKRIKREVDDLGPEVGDIKIIPLYSTLPPQQQQRIFEPPPPKKQNGAIGRKVVVS
  TNIAETSLTIDGVVFVIDPGFAKQKVYNPRIRVESLLVTAISKASAQQRAGRAGRTRPGKCFRLYTEKAY
  KTEMQDNTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALNDDGDLTELGS
  MMAEFPLDPQLAKMVIASCDYNCSNEVLSITAMLSVPQCFVRPTEAKKAADEAKMRFAHIDGDHLTLLNV
  YHAFKQNHESVQWCYDNFINYRSLMSADNVRQQLSRIMDRFNLPRRSTDFTSRDYYINIRKALVTGYFMQ
  VAHLERTGHYLTVKDNQVVQLHPSTVLDHKPEWVLYNEFVLTTKNYIRTCTDIKPEWLVKIAPQYYDMSN
  FPQCEAKRQLDRIIAKLQSKEYSQY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001358
• ORF Size: 2385 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001358.2, NP_001349.2
• RefSeq Size: 3019 bp
• RefSeq ORF: 2388 bp
• Locus ID: 1665
• Domains: DEAD, helicase_C, HA2
• Protein Pathways: Spliceosome
• MW: 90.9 kDa
• Gene Summary: The protein encoded by this gene is a putative ATP-dependent RNA helicase implicated in pre-mRNA splicing. [provided by RefSeq, Jul 2008]