MTP18 (MTFP1) (NM_016498) Human Tagged ORF Clone

CAT#: RC205922

MTFP1 (Myc-DDK-tagged)-Human mitochondrial fission process 1 (MTFP1), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Images

Coomassie blue staining of purified MTFP1 protein (Cat# TP305922). The protein was produced from HEK293T cells transfected with MTFP1 cDNA clone (Cat# RC205922) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MTFP1
• Synonyms: HSPC242; MTP18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205922 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGAGCCGCAGCCGCGGGGCGCAGAGCGCGATCTCTACCGGGACACGTGGGTGCGATACCTGGGCT
  ATGCCAATGAGGTGGGCGAGGCTTTCCGCTCTCTTGTGCCAGCGGCGGTGGTGTGGCTGAGCTATGGCGT
  GGCCAGCTCCTACGTGCTGGCGGATGCCATTGACAAAGGCAAGAAGGCTGGAGAGGTGCCCAGCCCTGAA
  GCAGGCCGCAGCGCCAGGGTGACCGTGGCTGTGGTGGACACCTTTGTATGGCAGGCTCTAGCCTCTGTGG
  CCATTCCGGGCTTCACCATCAACCGCGTGTGTGCTGCCTCTCTCTATGTCCTGGGCACTGCCACCCGCTG
  GCCCCTGGCTGTCCGCAAGTGGACCACCACCGCGCTTGGGCTGTTGACCATCCCCATCATTATCCACCCC
  ATTGACAGGTCGGTGGATTTCCTCCTGGACTCCAGCCTGCGCAAGCTCTACCCAACAGTGGGGAAGCCCA
  GCTCCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205922 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSEPQPRGAERDLYRDTWVRYLGYANEVGEAFRSLVPAAVVWLSYGVASSYVLADAIDKGKKAGEVPSPE
  AGRSARVTVAVVDTFVWQALASVAIPGFTINRVCAASLYVLGTATRWPLAVRKWTTTALGLLTIPIIIHP
  IDRSVDFLLDSSLRKLYPTVGKPSSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016498
• ORF Size: 498 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016498.3, NM_016498.4, NP_057582.2
• RefSeq Size: 1298 bp
• RefSeq ORF: 501 bp
• Locus ID: 51537
• MW: 18 kDa
• Gene Summary: MTP18 is a mitochondrial protein and downstream target of the phosphatidylinositol 3-kinase (see PIK3CA, MIM 171834) signaling pathway that plays a role in cell viability and mitochondrial dynamics (Tondera et al., 2004 [PubMed 15155745]). [supplied by OMIM, Mar 2008]