SMRP1 (C9orf24) (NM_032596) Human Tagged ORF Clone

CAT#: RC205959

C9orf24 (Myc-DDK-tagged)-Human chromosome 9 open reading frame 24 (C9orf24), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY410011) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205959 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified C9orf24 protein (Cat# TP305959). The protein was produced from HEK293T cells transfected with C9orf24 cDNA clone (Cat# RC205959) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C9orf24
• Synonyms: bA573M23.4; CBE1; NYD-SP22; SMRP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205959 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCTGTTCTCCCGTAAGACCAGGACCCCTATCAGTACCTACAGTGACTCCTACAGGGCTCCCACCT
  CCATCAAGGAGGTCTATAAGGACCCACCCCTGTGTGCCTGGGAAGCCAACAAGTTTCTGACTCCGGGTCT
  GACTCATACCATGGAGCGACATGTGGATCCCGAAGCCCTGCAGAAGATGGCCAAATGTGCTGTACAGGAC
  TACACTTATAGGGGGTCCATATCAGGCCACCCCTACTTGCCTGAGAAGTACTGGCTTTCTCAAGAGGAAG
  CAGACAAATGCAGCCCAAACTACCTGGGCAGTGACTGGTACAACACATGGAGGATGGAACCTTACAACAG
  CAGCTGCTGCAACAAGTATACCACCTACCTTCCTCGGCTGCCTAAGGAGGCCAGGATGGAGACAGCAGTT
  CGAGGAATGCCCTTGGAATGCCCTCCTAGGCCGGAGCGGCTCAATGCCTACGAGCGCGAAGTGATGGTGA
  ACATGCTGAACTCACTGTCGCGGAACCAGCAGCTGCCGCGGATCACGCCCCGATGCGGGTGCGTGGACCC
  GCTGCCCGGCCGCCTGCCCTTCCATGGTTACGAAAGTGCTTGCTCGGGCCGCCACTACTGTCTGCGCGGG
  ATGGACTACTACGCCAGCGGGGCGCCCTGCACCGACCGCCGCCTGCGGCCTTGGTGCCGGGAGCAACAGA
  CTATGTGTACCTCCCTACGAGCACCGGCCCGGAATGCAGTGTGCTGTTACAACTCCCCCGCCGTCATACT
  ACCCATATCCGAACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205959 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFLFSRKTRTPISTYSDSYRAPTSIKEVYKDPPLCAWEANKFLTPGLTHTMERHVDPEALQKMAKCAVQD
  YTYRGSISGHPYLPEKYWLSQEEADKCSPNYLGSDWYNTWRMEPYNSSCCNKYTTYLPRLPKEARMETAV
  RGMPLECPPRPERLNAYEREVMVNMLNSLSRNQQLPRITPRCGCVDPLPGRLPFHGYESACSGRHYCLRG
  MDYYASGAPCTDRRLRPWCREQQTMCTSLRAPARNAVCCYNSPAVILPISEP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032596
• ORF Size: 786 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032596.2, NM_032596.3, NP_115985.2
• RefSeq Size: 1184 bp
• RefSeq ORF: 789 bp
• Locus ID: 84688
• MW: 30.2 kDa
• Gene Summary: This gene encodes a nuclear- or perinuclear-localized protein with no predicted domains or similarity to other known proteins. Expression of this gene is induced during the differentiation of bronchial epithelial cells, and the encoded protein may play a role in ciliogenesis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]