RNF23 (TRIM39) (NM_172016) Human Tagged ORF Clone

CAT#: RC205987

TRIM39 (Myc-DDK-tagged)-Human tripartite motif containing 39 (TRIM39), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY406809) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205987 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TRIM39 protein (Cat# TP305987). The protein was produced from HEK293T cells transfected with TRIM39 cDNA clone (Cat# RC205987) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRIM39
• Synonyms: RNF23; TFP; TRIM39B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205987 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGACAAGTCTGTTAGAGGCTGGGGCCTCTGCAGCCTCTACAGCTGCGGCTTTGGAGAACTTAC
  AGGTGGAGGCGAGCTGCTCTGTGTGCCTGGAGTATCTGAAGGAACCTGTCATCATTGAGTGTGGGCACAA
  CTTCTGCAAAGCTTGCATCACCCGCTGGTGGGAGGACCTAGAGAGGGACTTCCCTTGTCCTGTCTGTCGA
  AAGACATCCCGCTACCGCAGTCTCCGACCTAATCGGCAACTAGGCAGTATGGTGGAAATTGCCAAGCAGC
  TCCAGGCCGTCAAGCGGAAGATCCGGGATGAGAGCCTCTGCCCCCAACACCATGAGGCCCTCAGCCTTTT
  CTGTTATGAGGACCAGGAGGCTGTATGCTTGATATGTGCAATTTCCCACACCCACCGGGCCCACACCGTT
  GTGCCACTGGACGATGCTACACAGGAGTACAAGGAAAAACTGCAGAAGTGTCTGGAGCCCCTGGAACAGA
  AGCTGCAGGAGATCACTCGCTGCAAGTCCTCTGAGGAGAAGAAGCCTGGTGAGCTCAAGAGACTAGTGGA
  AAGTCGCCGACAGCAGATCTTGAGGGAGTTTGAAGAGCTTCATAGGCGGCTGGATGAAGAGCAGCAGGTG
  TTGCTTTCACGACTGGAAGAAGAGGAACAGGACATTCTGCAGCGACTCCGAGAAAATGCTGCTCACCTTG
  GGGACAAGCGCCGGGACCTGGCCCACTTGGCTGCCGAGGTGGAGGGCAAGTGCTTACAGTCAGGCTTCGA
  GATGCTTAAGGATGTCAAAAGTACCCTGGAAAAATGTGAAAAGGTGAAGACCATGGAGGTGACTTCAGTA
  TCCATAGAGCTGGAAAAGAACTTCAGCAATTTTCCCCGACAGTACTTTGCCCTAAGGAAAATCCTTAAAC
  AGCTAATTGCGGATGTGACCCTGGACCCTGAGACAGCTCATCCTAACCTAGTCCTGTCAGAGGATCGTAA
  GAGCGTCAAGTTCGTGGAGACAAGACTCCGGGATCTCCCTGACACACCAAGGCGTTTCACCTTCTACCCT
  TGCGTCCTGGCTACTGAGGGTTTCACCTCAGGTCGACACTACTGGGAGGTGGAGGTGGGCGACAAGACCC
  ACTGGGCAGTGGGTGTATGCCGGGACTCCGTGAGCCGAAAGGGCGAGTTGACTCCACTCCCTGAGACTGG
  CTACTGGCGGGTGCGGCTATGGAATGGGGACAAATATGCAGCCACCACCACACCTTTTACCCCTTTGCAC
  ATCAAGGTGAAACCCAAGCGGGTAGGCATATTCCTAGACTATGAGGCCGGCACACTGTCTTTCTACAATG
  TCACAGACCGCTCTCATATCTACACCTTCACTGATACTTTTACTGAGAAACTTTGGCCCCTCTTCTACCC
  AGGCATCCGGGCTGGACGGAAGAATGCTGCACCACTTACCATCAGGCCCCCAACAGATTGGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205987 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAETSLLEAGASAASTAAALENLQVEASCSVCLEYLKEPVIIECGHNFCKACITRWWEDLERDFPCPVCR
  KTSRYRSLRPNRQLGSMVEIAKQLQAVKRKIRDESLCPQHHEALSLFCYEDQEAVCLICAISHTHRAHTV
  VPLDDATQEYKEKLQKCLEPLEQKLQEITRCKSSEEKKPGELKRLVESRRQQILREFEELHRRLDEEQQV
  LLSRLEEEEQDILQRLRENAAHLGDKRRDLAHLAAEVEGKCLQSGFEMLKDVKSTLEKCEKVKTMEVTSV
  SIELEKNFSNFPRQYFALRKILKQLIADVTLDPETAHPNLVLSEDRKSVKFVETRLRDLPDTPRRFTFYP
  CVLATEGFTSGRHYWEVEVGDKTHWAVGVCRDSVSRKGELTPLPETGYWRVRLWNGDKYAATTTPFTPLH
  IKVKPKRVGIFLDYEAGTLSFYNVTDRSHIYTFTDTFTEKLWPLFYPGIRAGRKNAAPLTIRPPTDWE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172016
• ORF Size: 1464 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172016.1, NM_172016.2, NP_742013.1
• RefSeq Size: 3578 bp
• RefSeq ORF: 1467 bp
• Locus ID: 56658
• Protein Families: Druggable Genome
• MW: 56.4 kDa
• Gene Summary: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The function of this protein has not been identified. This gene lies within the major histocompatibility complex class I region on chromosome 6. Alternate splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]