CDC2L6 (CDK19) (NM_015076) Human Tagged ORF Clone

CAT#: RC206105

CDK19 (Myc-DDK-tagged)-Human cyclin-dependent kinase 19 (CDK19)

Product Images

Western blot validation of overexpression lysate (Cat# LY414814) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206105 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CDK19 protein (Cat# TP306105). The protein was produced from HEK293T cells transfected with CDK19 cDNA clone (Cat# RC206105) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDK19
• Synonyms: bA346C16.3; CDC2L6; CDK11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206105 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTATGATTTCAAGGCGAAGCTGGCGGCGGAGCGGGAGCGGGTGGAGGATTTGTTTGAGTACGAAG
  GGTGCAAAGTGGGACGCGGCACCTACGGTCACGTCTACAAGGCGAGGCGGAAAGATGGAAAAGATGAAAA
  GGAATATGCATTGAAGCAAATTGAAGGCACAGGAATATCCATGTCGGCTTGTAGAGAGATTGCACTTTTG
  CGAGAATTGAAGCACCCTAATGTGATTGCATTGCAGAAGGTGTTCCTTTCTCACAGTGACAGGAAGGTAT
  GGCTGCTGTTTGATTATGCAGAGCATGACTTGTGGCATATTATTAAGTTTCACCGTGCATCAAAAGCAAA
  TAAAAAGCCCATGCAGTTGCCAAGATCTATGGTTAAATCCTTACTTTACCAGATTCTTGATGGTATCCAT
  TACCTCCATGCAAATTGGGTGCTTCACAGAGACTTGAAACCAGCAAATATCCTAGTAATGGGAGAAGGTC
  CTGAGAGGGGGAGAGTCAAAATAGCTGACATGGGTTTTGCCAGATTATTCAATTCTCCTCTAAAGCCACT
  AGCAGATTTGGATCCAGTAGTTGTGACATTTTGGTATCGGGCTCCAGAACTTTTGCTTGGTGCAAGGCAT
  TATACAAAGGCCATTGATATATGGGCAATAGGTTGTATATTTGCTGAATTGTTGACTTCGGAACCTATTT
  TTCACTGTCGTCAGGAAGATATAAAAACAAGCAATCCCTTTCATCATGATCAACTGGATCGGATATTTAG
  TGTCATGGGGTTTCCTGCAGATAAAGACTGGGAAGATATTAGAAAGATGCCAGAATATCCCACACTTCAA
  AAAGACTTTAGAAGAACAACGTATGCCAACAGTAGCCTCATAAAGTACATGGAGAAACACAAGGTCAAGC
  CTGACAGCAAAGTGTTCCTCTTGCTTCAGAAACTCCTGACCATGGATCCAACCAAGAGAATTACCTCGGA
  GCAAGCTCTGCAGGATCCCTATTTTCAGGAGGACCCTTTGCCAACATTAGATGTATTTGCCGGCTGCCAG
  ATTCCATACCCCAAACGAGAATTCCTTAATGAAGATGATCCTGAAGAAAAAGGTGACAAGAATCAGCAAC
  AGCAGCAGAACCAGCATCAGCAGCCCACAGCCCCTCCACAGCAGGCAGCAGCCCCTCCACAGGCGCCCCC
  ACCACAGCAGAACAGCACCCAGACCAACGGGACCGCAGGTGGGGCTGGGGCCGGGGTCGGGGGCACCGGA
  GCAGGGTTGCAGCACAGCCAGGACTCCAGCCTGAACCAGGTGCCTCCAAACAAGAAGCCACGGCTAGGGC
  CTTCAGGCGCAAACTCAGGTGGACCTGTGATGCCCTCGGATTATCAGCACTCCAGTTCTCGCCTGAATTA
  CCAAAGCAGCGTTCAGGGATCCTCTCAGTCCCAGAGCACACTTGGCTACTCTTCCTCGTCTCAGCAGAGC
  TCACAGTACCACCCATCTCACCAGGCCCACCGGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206105 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDYDFKAKLAAERERVEDLFEYEGCKVGRGTYGHVYKARRKDGKDEKEYALKQIEGTGISMSACREIALL
  RELKHPNVIALQKVFLSHSDRKVWLLFDYAEHDLWHIIKFHRASKANKKPMQLPRSMVKSLLYQILDGIH
  YLHANWVLHRDLKPANILVMGEGPERGRVKIADMGFARLFNSPLKPLADLDPVVVTFWYRAPELLLGARH
  YTKAIDIWAIGCIFAELLTSEPIFHCRQEDIKTSNPFHHDQLDRIFSVMGFPADKDWEDIRKMPEYPTLQ
  KDFRRTTYANSSLIKYMEKHKVKPDSKVFLLLQKLLTMDPTKRITSEQALQDPYFQEDPLPTLDVFAGCQ
  IPYPKREFLNEDDPEEKGDKNQQQQQNQHQQPTAPPQQAAAPPQAPPPQQNSTQTNGTAGGAGAGVGGTG
  AGLQHSQDSSLNQVPPNKKPRLGPSGANSGGPVMPSDYQHSSSRLNYQSSVQGSSQSQSTLGYSSSSQQS
  SQYHPSHQAHRY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015076
• ORF Size: 1506 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015076.1, NM_015076.2, NM_015076.3, NM_015076.4, NP_055891.1
• RefSeq Size: 6246 bp
• RefSeq ORF: 1509 bp
• Locus ID: 23097
• Protein Families: Druggable Genome, Protein Kinase
• MW: 56.8 kDa
• Gene Summary: This gene encodes a protein that is one of the components of the Mediator co-activator complex. The Mediator complex is a multi-protein complex required for transcriptional activation by DNA binding transcription factors of genes transcribed by RNA polymerase II. The protein encoded by this gene is similar to cyclin-dependent kinase 8 which can also be a component of the Mediator complex. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]