MCM4 (NM_182746) Human Tagged ORF Clone
CAT#: RC206122
MCM4 (Myc-DDK-tagged)-Human minichromosome maintenance complex component 4 (MCM4), transcript variant 2
"NM_182746" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | MCM4 |
Synonyms | CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD; P1-CDC21 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC206122 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTCCCCGGCGTCGACCCCGAGCCGCCGCGGCAGCCGGCGTGGAAGGGCCACCCCCGCCCAGACGC CTCGGAGTGAGGATGCCAGGTCATCTCCCTCTCAGAGACGTAGAGGCGAGGATTCCACCTCCACGGGGGA GTTGCAGCCGATGCCAACCTCGCCTGGAGTGGACCTGCAGAGCCCTGCTGCGCAGGACGTGCTGTTTTCC AGCCCTCCCCAAATGCATTCTTCAGCTATCCCTCTTGACTTTGATGTTAGTTCACCACTGACATACGGCA CTCCCAGCTCTCGGGTAGAGGGAACCCCAAGAAGTGGTGTTAGGGGCACACCTGTGAGACAGAGGCCTGA CCTGGGCTCTGCACAGAAGGGCCTGCAAGTGGATCTGCAGTCTGACGGGGCAGCAGCAGAAGATATAGTG GCAAGTGAGCAGTCTCTAGGCCAAAAACTTGTGATCTGGGGAACAGATGTAAATGTGGCAGCATGCAAAG AAAACTTTCAGAGATTTCTTCAGCGTTTTATTGACCCTCTGGCTAAAGAAGAAGAAAATGTTGGCATAGA TATTACTGAACCTCTATACATGCAACGACTTGGGGAGATTAATGTTATTGGTGAGCCATTTTTAAATGTG AACTGTGAACACATCAAATCATTTGACAAAAATTTGTACAGACAACTCATCTCTTACCCACAGGAAGTTA TTCCAACTTTTGACATGGCTGTCAATGAAATCTTCTTTGACCGTTACCCTGACTCAATCTTAGAACATCA GATTCAAGTAAGACCATTCAACGCATTGAAGACTAAGAATATGAGAAACCTGAATCCAGAAGACATTGAC CAGCTCATCACCATCAGCGGCATGGTGATCAGGACATCCCAGCTGATTCCCGAGATGCAGGAGGCCTTCT TCCAGTGCCAAGTGTGTGCCCACACGACCCGGGTGGAGATGGACCGCGGCCGCATTGCAGAGCCCAGTGT GTGCGGGCGCTGCCACACCACCCACAGCATGGCACTCATCCACAACCGCTCCCTCTTCTCTGACAAGCAG ATGATCAAGCTTCAGGAGTCTCCGGAAGACATGCCTGCAGGGCAGACACCACACACAGTTATCCTGTTTG CTCACAATGATCTCGTTGACAAGGTCCAGCCTGGGGACAGAGTGAATGTTACAGGCATCTATCGAGCTGT GCCTATTCGAGTCAATCCAAGAGTGAGTAATGTGAAGTCTGTCTACAAAACCCACATTGATGTCATTCAT TATCGGAAAACGGATGCAAAACGTCTGCATGGCCTTGATGAAGAAGCAGAACAGAAACTTTTTTCAGAGA AACGTGTGGAATTGCTTAAGGAACTTTCCAGGAAACCAGACATTTATGAGAGGCTTGCTTCAGCCTTGGC TCCAAGCATTTATGAACATGAAGATATAAAGAAGGGAATTTTGCTTCAGCTCTTTGGCGGGACAAGGAAG GATTTTAGTCACACTGGAAGGGGCAAATTTCGGGCTGAGATCAACATCTTGCTGTGTGGCGACCCTGGTA CCAGCAAGTCCCAGCTGCTGCAGTACGTGTACAACCTCGTCCCCAGGGGCCAGTACACGTCTGGGAAGGG CTCCAGTGCAGTTGGCCTCACTGCGTACGTAATGAAAGACCCTGAGACAAGGCAGCTGGTCCTGCAGACA GGTGCTCTTGTCCTGAGTGACAACGGCATCTGCTGTATCGATGAGTTCGACAAGATGAATGAAAGTACAA GATCGGTATTGCATGAAGTCATGGAACAGCAGACTCTGTCCATTGCAAAGGCTGGGATCATCTGTCAGCT CAATGCGCGCACCTCTGTCCTGGCAGCAGCAAATCCCATTGAGTCTCAGTGGAATCCTAAAAAAACAACC ATTGAAAACATCCAGCTGCCTCATACTTTATTATCAAGGTTTGATTTGATCTTCCTCATGCTGGACCCTC AGGACGAAGCCTATGACAGGCGTCTGGCTCACCACCTGGTCGCACTGTACTACCAGAGCGAGGAGCAGGC AGAGGAGGAGCTCCTGGACATGGCGGTGCTAAAGGACTACATTGCCTACGCGCACAGCACCATCATGCCG CGGCTAAGTGAGGAAGCCAGCCAGGCTCTCATCGAGGCTTATGTAGACATGAGGAAGATTGGCAGTAGCC GGGGAATGGTTTCTGCATACCCTCGACAGCTAGAGTCATTAATCCGCTTAGCAGAAGCCCATGCTAAAGT AAGATTGTCTAACAAAGTTGAAGCCATTGATGTGGAAGAGGCCAAACGCCTCCATCGGGAAGCTCTGAAG CAGTCTGCAACTGATCCCCGGACTGGCATCGTGGACATATCTATTCTTACTACGGGGATGAGTGCCACCT CTCGTAAACGGAAAGAAGAATTAGCTGAAGCATTGAAAAAGCTTATTTTATCTAAGGGCAAAACACCAGC TCTAAAATACCAGCAACTTTTTGAAGATATTCGGGGACAATCTGACATAGCAATTACTAAAGATATGTTT GAAGAAGCACTGCGTGCCCTGGCAGATGATGATTTCCTGACAGTGACTGGGAAGACCGTGCGCTTGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206122 protein sequence
Red=Cloning site Green=Tags(s) MSSPASTPSRRGSRRGRATPAQTPRSEDARSSPSQRRRGEDSTSTGELQPMPTSPGVDLQSPAAQDVLFS SPPQMHSSAIPLDFDVSSPLTYGTPSSRVEGTPRSGVRGTPVRQRPDLGSAQKGLQVDLQSDGAAAEDIV ASEQSLGQKLVIWGTDVNVAACKENFQRFLQRFIDPLAKEEENVGIDITEPLYMQRLGEINVIGEPFLNV NCEHIKSFDKNLYRQLISYPQEVIPTFDMAVNEIFFDRYPDSILEHQIQVRPFNALKTKNMRNLNPEDID QLITISGMVIRTSQLIPEMQEAFFQCQVCAHTTRVEMDRGRIAEPSVCGRCHTTHSMALIHNRSLFSDKQ MIKLQESPEDMPAGQTPHTVILFAHNDLVDKVQPGDRVNVTGIYRAVPIRVNPRVSNVKSVYKTHIDVIH YRKTDAKRLHGLDEEAEQKLFSEKRVELLKELSRKPDIYERLASALAPSIYEHEDIKKGILLQLFGGTRK DFSHTGRGKFRAEINILLCGDPGTSKSQLLQYVYNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQT GALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPIESQWNPKKTT IENIQLPHTLLSRFDLIFLMLDPQDEAYDRRLAHHLVALYYQSEEQAEEELLDMAVLKDYIAYAHSTIMP RLSEEASQALIEAYVDMRKIGSSRGMVSAYPRQLESLIRLAEAHAKVRLSNKVEAIDVEEAKRLHREALK QSATDPRTGIVDISILTTGMSATSRKRKEELAEALKKLILSKGKTPALKYQQLFEDIRGQSDIAITKDMF EEALRALADDDFLTVTGKTVRLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_182746 |
ORF Size | 2589 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_182746.1, NM_182746.2, NP_877423.1 |
RefSeq Size | 4800 bp |
RefSeq ORF | 2592 bp |
Locus ID | 4173 |
Cytogenetics | 8q11.21 |
Protein Families | Stem cell - Pluripotency, Transcription Factors |
Protein Pathways | Cell cycle, DNA replication |
MW | 96.6 kDa |
Gene Summary | 'The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC107549 | MCM4 (untagged)-Human minichromosome maintenance complex component 4 (MCM4), transcript variant 2 |
USD 760.00 |
|
RG206122 | MCM4 (GFP-tagged) - Human minichromosome maintenance complex component 4 (MCM4), transcript variant 2 |
USD 460.00 |
|
RC206122L1 | Lenti ORF clone of Human minichromosome maintenance complex component 4 (MCM4), transcript variant 2, Myc-DDK-tagged |
USD 768.00 |
|
RC206122L2 | Lenti ORF clone of Human minichromosome maintenance complex component 4 (MCM4), transcript variant 2, mGFP tagged |
USD 620.00 |
|
RC206122L3 | Lenti ORF clone of Human minichromosome maintenance complex component 4 (MCM4), transcript variant 2, Myc-DDK-tagged |
USD 620.00 |
|
RC206122L4 | Lenti ORF clone of Human minichromosome maintenance complex component 4 (MCM4), transcript variant 2, mGFP tagged |
USD 620.00 |
{0} Product Review(s)
Be the first one to submit a review