GPD1L (NM_015141) Human Tagged ORF Clone

CAT#: RC206131

GPD1L (Myc-DDK-tagged)-Human glycerol-3-phosphate dehydrogenase 1-like (GPD1L)

Product Images

Western blot validation of overexpression lysate (Cat# LY414689) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206131 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GPD1L protein (Cat# TP306131). The protein was produced from HEK293T cells transfected with GPD1L cDNA clone (Cat# RC206131) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPD1L
• Synonyms: GPD1-L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206131 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCGGCGCCCCTGAAAGTGTGCATCGTGGGCTCGGGGAACTGGGGTTCAGCTGTTGCAAAAATAA
  TTGGTAATAATGTCAAGAAACTTCAGAAATTTGCCTCCACAGTCAAGATGTGGGTCTTTGAAGAAACAGT
  GAATGGCAGAAAACTGACAGACATCATAAATAATGACCATGAAAATGTAAAATATCTTCCTGGACACAAG
  CTGCCAGAAAATGTGGTTGCCATGTCAAATCTTAGCGAGGCTGTGCAGGATGCAGACCTGCTGGTGTTTG
  TCATTCCCCACCAGTTCATTCACAGAATCTGTGATGAGATCACTGGGAGAGTGCCCAAGAAAGCGCTGGG
  AATCACCCTCATCAAGGGCATAGACGAGGGCCCCGAGGGGCTGAAGCTCATTTCTGACATCATCCGTGAG
  AAGATGGGTATTGACATCAGTGTGCTGATGGGAGCCAACATTGCCAATGAGGTGGCTGCAGAGAAGTTCT
  GTGAGACCACCATCGGCAGCAAAGTAATGGAGAACGGCCTTCTCTTCAAAGAACTTCTGCAGACTCCAAA
  TTTTCGAATTACCGTGGTTGATGATGCAGACACTGTTGAACTATGTGGTGCGCTTAAGAACATCGTAGCT
  GTGGGAGCTGGGTTCTGCGACGGCCTCCGCTGTGGAGACAACACCAAAGCGGCCGTCATCCGCCTGGGAC
  TCATGGAAATGATTGCTTTTGCCAGGATCTTCTGCAAAGGCCAAGTGTCTACAGCCACCTTCCTAGAGAG
  CTGCGGGGTGGCCGACCTGATCACCACCTGTTACGGAGGGCGGAACCGCAGGGTGGCCGAGGCCTTCGCC
  AGAACTGGGAAGACCATTGAAGAGTTGGAGAAGGAGATGCTGAATGGGCAAAAGCTCCAAGGACCGCAGA
  CTTCTGCTGAAGTGTACCGCATCCTCAAACAGAAGGGACTACTGGACAAGTTTCCATTGTTTACTGCAGT
  GTATCAGATCTGCTACGAAAGCAGACCAGTTCAAGAGATGTTGTCTTGTCTTCAGAGCCATCCAGAGCAT
  ACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206131 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAPLKVCIVGSGNWGSAVAKIIGNNVKKLQKFASTVKMWVFEETVNGRKLTDIINNDHENVKYLPGHK
  LPENVVAMSNLSEAVQDADLLVFVIPHQFIHRICDEITGRVPKKALGITLIKGIDEGPEGLKLISDIIRE
  KMGIDISVLMGANIANEVAAEKFCETTIGSKVMENGLLFKELLQTPNFRITVVDDADTVELCGALKNIVA
  VGAGFCDGLRCGDNTKAAVIRLGLMEMIAFARIFCKGQVSTATFLESCGVADLITTCYGGRNRRVAEAFA
  RTGKTIEELEKEMLNGQKLQGPQTSAEVYRILKQKGLLDKFPLFTAVYQICYESRPVQEMLSCLQSHPEH
  T
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015141
• ORF Size: 1053 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015141.1, NM_015141.2, NM_015141.3, NP_055956.1
• RefSeq Size: 4068 bp
• RefSeq ORF: 1056 bp
• Locus ID: 23171
• Domains: NAD_Gly3P_dh
• Protein Pathways: Glycerophospholipid metabolism
• MW: 38.4 kDa
• Gene Summary: The protein encoded by this gene catalyzes the conversion of sn-glycerol 3-phosphate to glycerone phosphate. The encoded protein is found in the cytoplasm, associated with the plasma membrane, where it binds the sodium channel, voltage-gated, type V, alpha subunit (SCN5A). Defects in this gene are a cause of Brugada syndrome type 2 (BRS2) as well as sudden infant death syndrome (SIDS). [provided by RefSeq, Jul 2010]