PLBD2 (NM_173542) Human Tagged ORF Clone

CAT#: RC206135

PLBD2 (Myc-DDK-tagged)-Human phospholipase B domain containing 2 (PLBD2), transcript variant 1

Product Images

Coomassie blue staining of purified PLBD2 protein (Cat# TP306135). The protein was produced from HEK293T cells transfected with PLBD2 cDNA clone (Cat# RC206135) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PLBD2
• Synonyms: P76
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206135 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGGCCAGATGTACTGCTACCCCGGCAGCCACCTGGCCCGGGCGCTGACGCGGGCGCTGGCGCTGG
  CCCTGGTGCTGGCCCTGCTGGTCGGGCCGTTCCTGAGCGGCCTGGCGGGGGCGATCCCAGCGCCGGGGGG
  CCGCTGGGCGCGCGATGGGCAGGTCCCTCCAGCCTCCCGCAGCCGCTCGGTGCTCCTGGACGTCTCGGCG
  GGCCAGCTGCTTATGGTGGACGGACGCCACCCTGACGCCGTGGCCTGGGCCAACCTCACCAACGCCATCC
  GCGAGACTGGGTGGGCCTTCCTGGAGCTGGGCACAAGTGGCCAATACAATGACAGCTTGCAGGCCTATGC
  AGCCGGTGTGGTGGAGGCTGCTGTGTCGGAGGAGCTCATCTACATGCACTGGATGAACACGGTGGTGAAT
  TACTGCGGCCCCTTCGAGTATGAAGTCGGCTACTGCGAGAGGCTGAAGAGCTTCCTGGAGGCCAACCTAG
  AGTGGATGCAGGAAGAGATGGAGTCAAACCCAGACTCACCTTACTGGCACCAGGTGCGGCTGACCCTCCT
  GCAGCTGAAAGGCCTGGAGGACAGCTACGAAGGCCGTGTGAGCTTCCCAGCTGGGAAGTTCACCATCAAA
  CCCTTGGGGTTCCTCCTGCTGCAGCTCTCTGGGGACCTGGAAGACCTGGAGCTGGCCCTGAACAAGACCA
  AGATCAAACCTTCTCTGGGCTCTGGCTCCTGTTCTGCCCTCATCAAGCTGCTCCCTGGCCAGAGTGACCT
  CCTGGTTGCCCACAACACCTGGAACAACTACCAGCACATGCTGCGTGTCATCAAGAAGTACTGGCTCCAG
  TTCCGGGAAGGCCCCTGGGGGGACTACCCGCTGGTTCCCGGCAACAAGCTGGTCTTCTCCTCCTACCCCG
  GCACCATCTTCTCCTGCGACGACTTCTACATCCTGGGCAGTGGGCTGGTGACACTGGAGACCACCATTGG
  CAACAAGAACCCAGCCCTGTGGAAGTATGTGCGGCCCAGGGGCTGTGTGCTGGAGTGGGTACGCAACATC
  GTGGCCAACCGCCTGGCCTCGGATGGGGCCACCTGGGCAGACATCTTCAAGAGGTTCAACAGCGGCACGT
  ATAACAACCAGTGGATGATCGTGGACTACAAGGCGTTCATCCCGGGTGGGCCCAGCCCCGGGAGCCGGGT
  GCTTACCATCCTGGAGCAGATCCCCGGCATGGTGGTGGTGGCTGACAAGACCTCGGAGCTCTACCAGAAG
  ACCTACTGGGCCAGCTACAACATACCGTCCTTCGAGACTGTGTTCAATGCCAGTGGGCTGCAGGCCCTAG
  TGGCCCAGTATGGGGACTGGTTTTCTTATGACGGGAGCCCCCGGGCCCAGATCTTCCGGCGGAACCAGTC
  ACTGGTACAAGACATGGACTCCATGGTCAGGCTGATGAGGTACAATGACTTCCTCCATGACCCTCTGTCA
  CTGTGCAAAGCCTGCAACCCCCAGCCCAATGGGGAGAATGCTATCTCCGCCCGCTCCGACCTCAACCCGG
  CCAATGGCTCCTACCCCTTCAAGGCCCTGCGTCAGCGCTCCCATGGGGGTATCGATGTGAAGGTGACCAG
  CATGTCACTGGCCAGGATCCTGAGCCTGCTGGCGGCCAGCGGTCCCACGTGGGACCAGGTGCCCCCGTTC
  CAGTGGAGCACCTCGCCCTTCAGCGGCCTGCTGCACATGGGCCAGCCAGACCTCTGGAAGTTCGCGCCTG
  TCAAGGTTTCATGGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206135 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVGQMYCYPGSHLARALTRALALALVLALLVGPFLSGLAGAIPAPGGRWARDGQVPPASRSRSVLLDVSA
  GQLLMVDGRHPDAVAWANLTNAIRETGWAFLELGTSGQYNDSLQAYAAGVVEAAVSEELIYMHWMNTVVN
  YCGPFEYEVGYCERLKSFLEANLEWMQEEMESNPDSPYWHQVRLTLLQLKGLEDSYEGRVSFPAGKFTIK
  PLGFLLLQLSGDLEDLELALNKTKIKPSLGSGSCSALIKLLPGQSDLLVAHNTWNNYQHMLRVIKKYWLQ
  FREGPWGDYPLVPGNKLVFSSYPGTIFSCDDFYILGSGLVTLETTIGNKNPALWKYVRPRGCVLEWVRNI
  VANRLASDGATWADIFKRFNSGTYNNQWMIVDYKAFIPGGPSPGSRVLTILEQIPGMVVVADKTSELYQK
  TYWASYNIPSFETVFNASGLQALVAQYGDWFSYDGSPRAQIFRRNQSLVQDMDSMVRLMRYNDFLHDPLS
  LCKACNPQPNGENAISARSDLNPANGSYPFKALRQRSHGGIDVKVTSMSLARILSLLAASGPTWDQVPPF
  QWSTSPFSGLLHMGQPDLWKFAPVKVSWD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173542
• ORF Size: 1767 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173542.1, NM_173542.2, NM_173542.3, NP_775813.1
• RefSeq Size: 2832
• RefSeq ORF: 1770
• Locus ID: 196463
• Protein Families: Transmembrane
• MW: 65.5 kDa