HMGB4 (NM_145205) Human Tagged ORF Clone

CAT#: RC206145

HMGB4 (Myc-DDK-tagged)-Human high mobility group box 4 (HMGB4), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY407944) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206145 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HMGB4
• Synonyms: dJ1007G16.5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206145 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAAAGAAATCCAGCTAAAGCCTAAGGCAAATGTCTCTTCTTACGTTCACTTTTTGCTGAATTACA
  GAAACAAATTCAAGGAGCAGCAGCCAAGTACCTACGTTGGCTTTAAAGAGTTCTCTAGAAAGTGTTCGGA
  AAAATGGAGATCCATCTCAAAGCATGAAAAGGCCAAATATGAAGCCCTGGCCAAACTCGACAAAGCCCGA
  TACCAGGAAGAAATGATGAATTATGTTGGCAAGAGGAAGAAACGGAGAAAGCGGGATCCCCAGGCACCCA
  GACGGCCTCCATCATCCTTCCTACTCTTCTGCCAAGACCACTATGCTCAGCTGAAGAGGGAGAACCCGAA
  CTGGTCGGTGGTGCAGGTGGCCAAGGCCACAGGGAAGATGTGGTCAACAGCGACAGACCTGGAGAAGCAC
  CCTTATGAGCAAAGAGTGGCTCTCCTGAGAGCTAAGTACTTCGAGGAACTTGAACTCTACCGTAAACAAT
  GTAATGCCAGGAAGAAGTACCGAATGTCAGCTAGAAACCGGTGCAGAGGGAAAAGAGTCAGGCAGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206145 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGKEIQLKPKANVSSYVHFLLNYRNKFKEQQPSTYVGFKEFSRKCSEKWRSISKHEKAKYEALAKLDKAR
  YQEEMMNYVGKRKKRRKRDPQAPRRPPSSFLLFCQDHYAQLKRENPNWSVVQVAKATGKMWSTATDLEKH
  PYEQRVALLRAKYFEELELYRKQCNARKKYRMSARNRCRGKRVRQS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145205
• ORF Size: 558 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_145205.1, NM_145205.2, NM_145205.3, NM_145205.4, NM_145205.5, NP_660206.2
• RefSeq Size: 2352
• RefSeq ORF: 561
• Locus ID: 127540
• Protein Families: Transcription Factors
• MW: 22.4 kDa
• Gene Summary: Belongs to the HMGB family. [UniProtKB/Swiss-Prot Function]