RAB11FIP5 (NM_015470) Human Tagged ORF Clone

CAT#: RC206173

RAB11FIP5 (Myc-DDK-tagged)-Human RAB11 family interacting protein 5 (class I) (RAB11FIP5)

Product Images

Western blot validation of overexpression lysate (Cat# LY414522) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206173 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified RAB11FIP5 protein (Cat# TP306173). The protein was produced from HEK293T cells transfected with RAB11FIP5 cDNA clone (Cat# RC206173) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RAB11FIP5
• Synonyms: GAF1; pp75; RIP11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206173 representing NM_015470
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGGTGCGGGGCGCGGAGCCGGCGGCGGGGCCTTCCCGCTGGCTGCCCACGCACGTCCAGGTGA
  CGGTGCTGCGGGCCCGCGGGCTGCGGGGCAAGAGCTCGGGAGCGGGCAGCACCAGCGACGCGTACACGGT
  GATCCAGGTGGGCCGCGAGAAGTACAGTACGTCGGTGGTGGAGAAGACGCACGGCTGTCCCGAGTGGCGT
  GAGGAGTGCTCCTTCGAGCTGCCGCCGGGGGCCCTGGATGGCCTGCTGCGGGCGCAGGAGGCCGACGCGG
  GCCCGGCGCCCTGGGCCGCGAGCTCCGCCGCCGCCTGCGAGCTGGTGCTCACCACCATGCACCGCTCGCT
  CATCGGCGTCGACAAGTTCCTGGGCCAGGCCACGGTGGCGCTGGACGAGGTCTTCGGCGCAGGCCGCGCC
  CAGCACACGCAGTGGTACAAGCTGCACTCCAAGCCAGGCAAGAAGGAGAAGGAACGCGGCGAGATTGAAG
  TCACCATCCAGTTCACGCGCAACAACCTGAGCGCCATGTTTGACCTGTCCATGAAGGACAAGCCAAGGTC
  TCCCTTCAGCAAGATCAGGGACAAGATGAAGGGCAAGAAGAAGTATGATCTGGAATCTGCCTCTGCCATC
  CTCCCAAGCAGCGCCATAGAGGATCCTGACCTGGGCAGCCTGGGCAAGATGGGCAAAGCCAAAGGCTTCT
  TCCTCCGCAACAAGCTGCGCAAGTCGTCCCTGACCCAGTCCAACACCTCGCTGGGCTCGGACAGCACCCT
  GTCCTCAGCCAGCGGGAGCTTGGCCTACCAGGGACCTGGCGCCGAACTCCTCACCCGCTCACCAAGCCGT
  AGCAGCTGGCTGTCCACTGAAGGGGGCAGGGACTCTGCACAGTCCCCCAAGCTGTTCACCCATAAGAGGA
  CCTACAGCGATGAGGCCAACCAGATGCGAGTGGCTCCTCCTCGGGCCCTTCTGGACCTTCAGGGCCACCT
  GGATGCTGCCTCCCGCTCTTCGCTCTGTGTCAATGGGAGCCACATTTACAATGAGGAGCCCCAGGGCCCT
  GTGCGGCACCGCAGCTCCATCTCGGGCTCGCTTCCATCCTCTGGCTCCTTGCAAGCTGTCTCTTCCCGGT
  TCTCCGAGGAGGGGCCTCGTTCCACAGATGACACCTGGCCCAGAGGCAGTCGTAGCAACAGCAGCTCAGA
  GGCAGTGCTTGGACAGGAGGAGCTGAGTGCTCAGGCTAAAGTCCTGGCCCCTGGGGCCAGCCACCCTGGA
  GAGGAGGAGGGGGCCCGGCTACCAGAGGGCAAGCCAGTCCAGGTTGCCACACCCATAGTGGCCTCCTCTG
  AGGCTGTGGCAGAGAAGGAGGGAGCCCGGAAGGAGGAACGCAAGCCCCGGATGGGTCTCTTCCACCACCA
  CCACCAAGGCCTAAGTCGGAGCGAGTTGGGTCGCCGAAGCTCTCTGGGGGAAAAGGGGGGTCCCATCCTG
  GGGGCCTCCCCACATCACTCATCCAGTGGGGAGGGAAAGGCCAAGAGTAGCTGGTTTGGCTTGAGAGAAG
  CCAAGGACCCGACTCAGAAACCCAGTCCCCACCCCGTGAAGCCCCTCAGTGCCGCCCCTGTGGAGGGCAG
  CCCCGACAGGAAGCAGTCCCGCTCCAGTCTGAGCATAGCCCTGAGCAGTGGGCTGGAGAAGCTCAAAACA
  GTCACATCTGGGAGCATTCAGCCTGTGACCCAGGCCCCCCAGGCTGGCCAGATGGTGGACACCAAAAGGC
  TGAAGGACTCAGCTGTGCTGGACCAGTCGGCCAAGTACTACAACCTGACCCACGATGAGCTCATCAGCCT
  GCTCCTGCAGCGGGAGCGGGAGCTGAGCCAGCGGGACGAGCATGTGCAGGAGCTGGAGAGCTACATCGAC
  CGGCTGCTGGTGCGGATCATGGAGACCTCACCCACGCTGCTGCAGATCCCCCCGGGCCCCCCCAAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206173 representing NM_015470
  Red=Cloning site Green=Tags(s)
  
  MALVRGAEPAAGPSRWLPTHVQVTVLRARGLRGKSSGAGSTSDAYTVIQVGREKYSTSVVEKTHGCPEWR
  EECSFELPPGALDGLLRAQEADAGPAPWAASSAAACELVLTTMHRSLIGVDKFLGQATVALDEVFGAGRA
  QHTQWYKLHSKPGKKEKERGEIEVTIQFTRNNLSAMFDLSMKDKPRSPFSKIRDKMKGKKKYDLESASAI
  LPSSAIEDPDLGSLGKMGKAKGFFLRNKLRKSSLTQSNTSLGSDSTLSSASGSLAYQGPGAELLTRSPSR
  SSWLSTEGGRDSAQSPKLFTHKRTYSDEANQMRVAPPRALLDLQGHLDAASRSSLCVNGSHIYNEEPQGP
  VRHRSSISGSLPSSGSLQAVSSRFSEEGPRSTDDTWPRGSRSNSSSEAVLGQEELSAQAKVLAPGASHPG
  EEEGARLPEGKPVQVATPIVASSEAVAEKEGARKEERKPRMGLFHHHHQGLSRSELGRRSSLGEKGGPIL
  GASPHHSSSGEGKAKSSWFGLREAKDPTQKPSPHPVKPLSAAPVEGSPDRKQSRSSLSIALSSGLEKLKT
  VTSGSIQPVTQAPQAGQMVDTKRLKDSAVLDQSAKYYNLTHDELISLLLQRERELSQRDEHVQELESYID
  RLLVRIMETSPTLLQIPPGPPK
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015470
• ORF Size: 1770 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015470.1, NM_015470.2, NP_056285.1
• RefSeq Size: 4340
• RefSeq ORF: 1962
• Locus ID: 26056
• Protein Pathways: Endocytosis
• MW: 70.2 kDa